CAJ | 학술논문

目的：研究甘草改善睡眠作用的药效物质基础。方法取Wistar大鼠30只，平均分为3组，分别为正常对照组、甘草冻干粉组、甘草次酸组。称取冻干粉20 g，加水溶解配成0.5 kg· L－1溶液，灌胃给药，正常组给予等体积蒸馏水。每天1次，连续7 d。用HPLC方法测定大鼠血清和脑脊液中移行成分。结果通过分析比较，确定甘草改善睡眠作用的药效物质基础是血清中甘草的移行成分甘草次酸。甘草次酸可使脑脊液中内源性物质的峰面积高于空白脑脊液峰面积，前者约是后者的8.6倍。结论甘草主要化学成分甘草次酸能明显促进脑脊液中内源性物质的分泌，从而达到改善睡眠作用。
목적：연구감초개선수면작용적약효물질기출。방법취Wistar대서30지，평균분위3조，분별위정상대조조、감초동간분조、감초차산조。칭취동간분20 g，가수용해배성0.5 kg· L－1용액，관위급약，정상조급여등체적증류수。매천1차，련속7 d。용HPLC방법측정대서혈청화뇌척액중이행성분。결과통과분석비교，학정감초개선수면작용적약효물질기출시혈청중감초적이행성분감초차산。감초차산가사뇌척액중내원성물질적봉면적고우공백뇌척액봉면적，전자약시후자적8.6배。결론감초주요화학성분감초차산능명현촉진뇌척액중내원성물질적분비，종이체도개선수면작용。
Objective To study the pharmacodynamic material basis of licorice andimprove the role of sleep.Methods Thirty Wistar rats were divided into 3 groups, which were normal control group, licorice powder group, glycyrrhetinic acid group.The freeze-dried powder 20 g, water dissolved into 0.5 kg· L-1 solution, administered orally, the normal con-trol group were given equal volume of distilled water.One times a day, continuous 7 d.Blood transitional component were analzed by HPLC method.Results Glycyrrhetinic acid could make the peak area of endog-enous substances in cerebrospinal fluid ( CSF ) higher than the peak area of the blank, the peak area ratio of glycyrrhizin could make the endoge-nous substances in cerebrospinal fluid in the area is about 8.6 times peak area of the blank CSF.That the main chemical components of licorice glycyrrhetinic acid can significantly promote the secretion of endogenous substances in the cerebrospinal fluid, so as to improve the role of sleep.Conclusion By analysis and comparison, which indicates that the active constituent( glycyrrhetinic acid) in licorice are confirmed and could im-prove the sedative function.