CAJ | 학술논문

目的：观察霞草苷对人结肠癌HT－29细胞凋亡的影响及其可能机制。方法用MTT法和流式细胞技术检测浓度为5，10，20，40μmol· L－1霞草苷对HT－29细胞生长、凋亡的影响；用实时荧光定量RT－PCR检测细胞Caspase－3、Bax的mRNA水平。结果霞草苷对HT－29细胞的生长有明显的增殖抑制作用，呈浓度和时间依赖性，细胞凋亡率明显增高。 Caspase－3和Bax的mRNA表达量逐渐增加，呈明显的浓度依赖性。结论霞草苷能诱导体外培养的人结肠癌HT－29细胞凋亡，上调Bax表达，活化Caspase－3可能是霞草苷诱导HT－29细胞凋亡的机制之一。
목적：관찰하초감대인결장암HT－29세포조망적영향급기가능궤제。방법용MTT법화류식세포기술검측농도위5，10，20，40μmol· L－1하초감대HT－29세포생장、조망적영향；용실시형광정량RT－PCR검측세포Caspase－3、Bax적mRNA수평。결과하초감대HT－29세포적생장유명현적증식억제작용，정농도화시간의뢰성，세포조망솔명현증고。 Caspase－3화Bax적mRNA표체량축점증가，정명현적농도의뢰성。결론하초감능유도체외배양적인결장암HT－29세포조망，상조Bax표체，활화Caspase－3가능시하초감유도HT－29세포조망적궤제지일。
Objective To investigate the effects of Gypsophila oldhami-ana gypsogenin( GOG) on induction of apoptosis in human colon cancer HT-29 cells.Methods The growth and apoptosis of HT -29 cells treated with 5,10,20,40 μmol· L-1 GOG are investigated by MTT assay and flow cytometry.The mRNA expression of Caspase-3 and Bax was measured by real-time fluorescence quantitative RT-PCR.Results GOG inhibited the proliferation of HT -29 cell line in a dose -and time-dependent manner. Cell apoptosis rate increase obviously.Caspase-3 and Bax mRNA expression quantity increase gradually, assumes the obvious density dependence.Conclusion GOG could inhibit HT-29 cell proliferation through inducing cell apoptosis in vitro.The mechanism might be that GOG could enhance the level of Bax and activate the pathway of Caspase-3.