CAJ | 학술논문

目的：用代谢组学研究儿童真性性早熟（ CPP）的代谢标记物，以探讨儿童性早熟的发病机制。方法用高效液相色谱－四级杆飞行时间质谱联用系统（ HPLC－QTOF－MS）对性早熟组和对照组儿童的血液样本进行代谢图谱分析，并结合主成分分析法（PCA）和偏最小二乘辨别分析法（PLS－DA），根据PLS分析的载荷图和VIP值，筛选24个代谢标记物，并对其参与的代谢途径进行分析。结果氨基酸、5－羟色胺、儿茶酚胺及脂代谢等多条代谢途径异常与儿童性早熟发生相关。此外，一些环境内分泌干扰物也可能导致性早熟。结论性早熟儿童和正常儿童血液代谢特征存在明显差异。
목적：용대사조학연구인동진성성조숙（ CPP）적대사표기물，이탐토인동성조숙적발병궤제。방법용고효액상색보－사급간비행시간질보련용계통（ HPLC－QTOF－MS）대성조숙조화대조조인동적혈액양본진행대사도보분석，병결합주성분분석법（PCA）화편최소이승변별분석법（PLS－DA），근거PLS분석적재하도화VIP치，사선24개대사표기물，병대기삼여적대사도경진행분석。결과안기산、5－간색알、인다분알급지대사등다조대사도경이상여인동성조숙발생상관。차외，일사배경내분비간우물야가능도치성조숙。결론성조숙인동화정상인동혈액대사특정존재명현차이。
Objective To apply metabolomics studies to find out meta-bolic markers for further understanding the pathogenesis of children cen-tral precocious puberty ( CPP ).Methods A high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrom-etry method ( HPLC-QTOF-MS) was used to figure out the metabolic characteristic of the CPP children and healthy children.Combined with the principal component analysis ( PCA) and partial least squares-diac-riminant analysis ( PLS-DA) , the major metabolic changes in peripher-al blood between the normal and onset group were statistically analyzed.According to the loading plot and variable importance plot ( VIP ) of PLS, twenty-four metabolic markers were identified.Furthermore, the pathways mediated by these metabolic markers were analyzed.Results CPP is closely related to the abnormalities of multiple metabolic path-ways, including amino acid, catecholamine, serotonin, and lipid metab-olism.Additionally, environmental endocrine disruptors play an impor-tant role in the occurrence of CPP.These findings provide a novel insight to the pathogenesis of CPP.Conclusion The metabolic characteristic of the CPP children was obviously different from that of the normal children.