CAJ | 학술논문

目的：评价支链 DNA （ bDNA ）技术检测高危人乳头瘤病毒外显子6／7（HPV E6／E7）mRNA对宫颈癌筛查的意义。方法用bDNA技术在335例液基细胞学标本（经活检确认病理92例）检测HPV E6／E7 mRNA，其中93例同时检测E6／E7 DNA；对mRNA在不同病理级别分布情况进行分析。结果随着宫颈病变程度加重，HPV E6／E7 mRNA表达率相应增加。 E6／E7 DNA与RNA间受试者工作特征曲线（ ROC ）分析，预测 CIN2＋病变的最佳工作点为530.53 copies· mL－1，ROC曲线下面积为0.789。结论 bDNA技术检测在液基细胞学标本中可很好地检测HPV E6／E7 mRNA。高危HPV E6／E7 mRNA检测的良好阳性似然比可用于宫颈癌筛查，其拷贝值可作为预测宫颈病变的指标。
목적：평개지련 DNA （ bDNA ）기술검측고위인유두류병독외현자6／7（HPV E6／E7）mRNA대궁경암사사적의의。방법용bDNA기술재335례액기세포학표본（경활검학인병리92례）검측HPV E6／E7 mRNA，기중93례동시검측E6／E7 DNA；대mRNA재불동병리급별분포정황진행분석。결과수착궁경병변정도가중，HPV E6／E7 mRNA표체솔상응증가。 E6／E7 DNA여RNA간수시자공작특정곡선（ ROC ）분석，예측 CIN2＋병변적최가공작점위530.53 copies· mL－1，ROC곡선하면적위0.789。결론 bDNA기술검측재액기세포학표본중가흔호지검측HPV E6／E7 mRNA。고위HPV E6／E7 mRNA검측적량호양성사연비가용우궁경암사사，기고패치가작위예측궁경병변적지표。
Objective To investigate the role of testing E6/E7 mRNA of high risk human papilloma virus ( HPV) for cervical cancer screening by branched DNA(bDNA)technology.Methods Branched DNA(bD-NA) technology was used to detect high-risk HPV E6/E7 mRNA and DNA directly from 335 liquid cytologic test(LCT) samples(91 samples were recognized by histopathology).Both of mRNA and DNA were detec-ted in 93 samples.The data according to cytological grades or pathology were statistically analyzed.Results The expression level of HPV E6/E7 mRNA transcripts elevated corresponding to the degree of cervical dyspla-sia.Receiver operating characteristic( ROC) curve was analyzed for pre-dicting CIN2 +, the optimal operating point value was 530.53 copies· mL-1.Conclusion bDNA technology can be used to detect HPV E6/E7 mRNA well in LCT samples.HPV E6/E7 mRNA test could be a useful tool for cervical cancer screening for its positive likelihood ratio.The copies of mRNA may be used to pool out high-risk patient in cervical cancer screening.