CAJ | 학술논문

目的：现代药理研究证实，炙甘草汤总提物、单个有效成分及其配伍能明显减少心律失常的出现。文中旨在将血清药理学方法结合电生理技术研究炙甘草汤含药血清治疗心房颤动的机制。方法将新西兰大白兔分为正常对照组、正常血清对照组、炙甘草汤含药血清组、炙甘草汤水煎液组，每组8只。建立心房颤动动兔模型后，检测右心耳组织场电位时程（ field action potential duration， fAPD），并观察炙甘草汤含药血清和水煎液对右心耳fAPD的作用。结果成功建立快速起搏右心房诱导心房颤动兔模型，起搏12 h后fAPD比起搏前明显缩短[（174．30±1．36）ms vs （162．48±0．88）ms， P＜0．05]。给予10％、15％、20％、25％含药血清和水煎液之后，均可作用于心房颤动兔右心耳组织fAPD导致fAPD延长，量效成正相关。10％、15％、20％、25％含药血清组fAPD分别为[（170．81±0．61）、（171．00±0．46）、（179．08±0．67）、（179．76±2．26）ms]较水煎液组fAPD [（163．82±0．78）、（163．66±0．95）、（174．06±1．32）、（176．84±1．19）ms]延长明显（P＜0．05）。结论 fAPD可作为心肌电生理特性改变的指标之一，10％～25％炙甘草汤含药血清可导致心房颤动兔fAPD延长可能是抗心房颤动发生的电生理机制。
목적：현대약리연구증실，자감초탕총제물、단개유효성분급기배오능명현감소심률실상적출현。문중지재장혈청약이학방법결합전생리기술연구자감초탕함약혈청치료심방전동적궤제。방법장신서란대백토분위정상대조조、정상혈청대조조、자감초탕함약혈청조、자감초탕수전액조，매조8지。건립심방전동동토모형후，검측우심이조직장전위시정（ field action potential duration， fAPD），병관찰자감초탕함약혈청화수전액대우심이fAPD적작용。결과성공건립쾌속기박우심방유도심방전동토모형，기박12 h후fAPD비기박전명현축단[（174．30±1．36）ms vs （162．48±0．88）ms， P＜0．05]。급여10％、15％、20％、25％함약혈청화수전액지후，균가작용우심방전동토우심이조직fAPD도치fAPD연장，량효성정상관。10％、15％、20％、25％함약혈청조fAPD분별위[（170．81±0．61）、（171．00±0．46）、（179．08±0．67）、（179．76±2．26）ms]교수전액조fAPD [（163．82±0．78）、（163．66±0．95）、（174．06±1．32）、（176．84±1．19）ms]연장명현（P＜0．05）。결론 fAPD가작위심기전생리특성개변적지표지일，10％～25％자감초탕함약혈청가도치심방전동토fAPD연장가능시항심방전동발생적전생리궤제。
[Abstract ] Objective Modern pharmacological studies confirmed Zhigancao Decoction total extract, single active ingredients and their combinations could obviously inhibit arrhythmia.This study was to investigate the effects of Zhigancao Decoction medicated serum combined with myocardial tissue/silicon substrate microelectrode arrays (MEA) on rapid atrial pacing(RAP). Methods New Zealand white rabbits were randomly divided into normal control group, normal serum control group, Zhigancao Decoction medica-ted serum group and water decoction group, 8 in each group.After the establishment of an atrial fibrillation rabbit model, the field ac-tion potential duration ( fAPD) of the right atrial appendage ( RAA ) tissue was measured and the effections of Zhigancao Decoction medicated serum and water decoction on the fAPD of RAA were observed. Results The successful modeling of rapid atrial pacing induced atrial fibrillation in rabbits contributed to the significant shortening of fAPD 12 h after pacing compared to that before pacing ([174.30 ±1.36]ms vs[162.48 ±0.88]ms, P<0.05).After giving 10%~25% Zhigancao Decoction medicated serum and water decoction, the fAPDs of RAA tissue in rabbits with atrial fibrillation were prolonged, which represented positive dose-response relation-ship.The fAPDs of the rabbits given serum containing 10%, 15%, 20%and 25%Zhigancao Decoction were respectively (170.81 ± 0.61)ms, (171.00 ±0.46)ms, (179.08 ±0.67)ms, (179.76 ±2.26)ms, which were longer than those of water decoction group ([163.82 ±0.780]ms, [163.66 ±0.95]ms, [174.06 ±1.32]ms, [176.84 ±1.19]ms), and the difference was statistically sig-nificant (P<0.05). Conclusion The fAPD can be taken as one index of cardiac electrophysiological change, and 10%~25%Zh-igancao Decoction medicated serum can lead to fAPD extension in rabbit model of atrial fibrillation, which might be the electrophysio-logical mechanism of anti-atrial fibrillation.