沙漠干热环境中暑大鼠肺损伤相关研究
사막간열배경중서대서폐손상상관연구
Lung injury in heatstroke rats in xerothermic environment of desert
  • 万方数据
    医学研究生学报 의학연구생학보
    JOURNAL OF MEDICAL POSTGRADUATE
    2014年 11期 1148-1151 ,共4页

    张波%刘江伟%张东%黄泼泼%杨帆

    장파%류강위%장동%황발발%양범

    沙漠%干热环境%中暑%肺损伤%大鼠 沙漠%榦熱環境%中暑%肺損傷%大鼠
    사막%간열배경%중서%폐손상%대서
    Desert%Xerothermic environment%Heatstroke%Lung injury%Rat
    目的：沙漠的极端干热环境极易引起中暑进而导致多器官损伤，目前国内外关于沙漠干热环境中暑相关肺损伤的研究报道较少。文中探讨沙漠干热环境中暑大鼠肺损伤性变化。方法将48只SPF级健康雄性SD大鼠按随机数字列表法分成6组，轻度中暑组（温度41℃、湿度10％，暴露70 min）及其对照组（温度25℃、湿度35％，暴露70 min），中度中暑组（温度41℃、湿度10％，暴露110 min）及其对照组（温度25℃、湿度35％，暴露110 min），重度中暑组（温度41℃、湿度10％，暴露145 min）及其对照组（温度25℃、湿度35％，暴露145 min）。采集中暑组及其对照组大鼠肺泡灌洗液、肺组织。行肺组织病理学观察并进行肺损伤评分，计算肺组织湿／干重量比（ W／D），检测大鼠肺灌洗液蛋白，测定肺组织中丙二醛（ malondialde-hyde， MDA）含量。结果病理组织学观察可见中暑组大鼠肺损伤病理变化均较其对照组严重，且随着热暴露时间的延长而逐渐加重；轻、中、重度中暑组肺损伤病理评分[（3．25±0．50）、（6．50±0．58）、（8．00±0．82）分]较其对照组[（1．25±0．50）、（1．00±0．00）、（1．25±0．50）分]明显升高（P＜0．05），肺W／D[（3．50±0．57）、（4．85±0．19）、（4．10±0．07）]较其对照组[（1．78±0．66）、（1．67±0．68）、（1．67±0．25）]明显升高（P＜0．05），肺灌洗液总蛋白含量[（93．51±5．54）、（141．24±6．07）、（160．76±6．74）μg／mL]较其对照组[（42．6±8．67）、（45．25±6．78）、（41．68±3．62）μg／mL]明显升高（P＜0．05）、MDA含量[（1．79±0．08）、（2．79±0．11）、（3．52±0．36） nmol／mgprot]较其对照组[（1．21±0．12）、（1．20±0．12）、（1．28±0．19）nmol／mgprot]明显升高（P＜0．05）。肺病理评分、灌洗液蛋白含量、MDA随中暑程度的加重而加重；肺W／D于中度中暑阶段最高。肺W／D、肺灌洗液总蛋白量、肺组织MDA含量与肺损伤病理评分间均呈正相关（ r＝0．989， P＜0．01）。结论沙漠干热环境中暑可造成大鼠肺损伤，随着中暑程度加重而逐渐加重，沙漠干热环境下中暑的治疗应注意加强对肺损伤的预防和保护。
    목적：사막적겁단간열배경겁역인기중서진이도치다기관손상，목전국내외관우사막간열배경중서상관폐손상적연구보도교소。문중탐토사막간열배경중서대서폐손상성변화。방법장48지SPF급건강웅성SD대서안수궤수자렬표법분성6조，경도중서조（온도41℃、습도10％，폭로70 min）급기대조조（온도25℃、습도35％，폭로70 min），중도중서조（온도41℃、습도10％，폭로110 min）급기대조조（온도25℃、습도35％，폭로110 min），중도중서조（온도41℃、습도10％，폭로145 min）급기대조조（온도25℃、습도35％，폭로145 min）。채집중서조급기대조조대서폐포관세액、폐조직。행폐조직병이학관찰병진행폐손상평분，계산폐조직습／간중량비（ W／D），검측대서폐관세액단백，측정폐조직중병이철（ malondialde-hyde， MDA）함량。결과병리조직학관찰가견중서조대서폐손상병리변화균교기대조조엄중，차수착열폭로시간적연장이축점가중；경、중、중도중서조폐손상병리평분[（3．25±0．50）、（6．50±0．58）、（8．00±0．82）분]교기대조조[（1．25±0．50）、（1．00±0．00）、（1．25±0．50）분]명현승고（P＜0．05），폐W／D[（3．50±0．57）、（4．85±0．19）、（4．10±0．07）]교기대조조[（1．78±0．66）、（1．67±0．68）、（1．67±0．25）]명현승고（P＜0．05），폐관세액총단백함량[（93．51±5．54）、（141．24±6．07）、（160．76±6．74）μg／mL]교기대조조[（42．6±8．67）、（45．25±6．78）、（41．68±3．62）μg／mL]명현승고（P＜0．05）、MDA함량[（1．79±0．08）、（2．79±0．11）、（3．52±0．36） nmol／mgprot]교기대조조[（1．21±0．12）、（1．20±0．12）、（1．28±0．19）nmol／mgprot]명현승고（P＜0．05）。폐병리평분、관세액단백함량、MDA수중서정도적가중이가중；폐W／D우중도중서계단최고。폐W／D、폐관세액총단백량、폐조직MDA함량여폐손상병리평분간균정정상관（ r＝0．989， P＜0．01）。결론사막간열배경중서가조성대서폐손상，수착중서정도가중이축점가중，사막간열배경하중서적치료응주의가강대폐손상적예방화보호。
    Objective The extremely xerothermic environment of desert easily causes heatstroke and consequently multiple organ damage.Few reports are seen at home and abroad on heatstroke-induced lung injury associated with dry heat of desert.This stud-y explored the changes of the injured lungs in heatstroke rats in xerothermic desert. Methods Forty-eight male SD rats were ran-domly divided into six groups of equal number: mild, moderate and severe heatstroke groups and their corresponding controls.The rats of the three heatstroke groups were exposed to a temperature of 41 ℃and humidity of 10%, while those in the three corresponding control groups to a temperature of 25 ℃ and humidity of 35% for 70, 110 and 145 minutes, respectively.Then all the rats were sacrificed and the bronchoalveolar lavage fluid ( BALF) and lung tissues were collected for observation of pathological changes, obtainment of lung in-jury scores, calculation of the wet/dry weight ratio of the lungs, measurement of the total protein level in the BALF, and determination of the malondialdehyde ( MDA) content in the lung tissue. Results The mild, moderate and severe heatstroke groups showed re-markable increases compared with the three corresponding control groups in lung injury scores (3.25 ±0.50, 6.50 ±0.58 and 8 ± 0.82 vs 1.25 ±0.50, 1.00 ±0.00 and 1.25 ±0.50), the wet/dry weight ratio of the lungs (3.50 ±0.57, 4.85 ±0.19 and 4.10 ± 0.07 vs 1.78 ±0.66, 1.67 ±0.68 and 1.67 ±0.25), the total protein level in the BALF ([93.51 ±5.54], [141.24 ±6.07] and [160.76 ±6.74] μg/mL vs [42.6 ±8.67], [45.25 ±6.78] and [41.68 ±3.62] μg/mL), and the content of MDA in the lung tis-sue ([1.79 ±0.08], [2.79 ±0.11] and [3.52 ±0.36] nmol/mg prot vs [1.21 ±0.12], [1.20 ±0.12] and [1.28 ±0.19] nmol/mg prot) .The lung injury scores, the total protein level in the BALF, and the content of MDA in the lung tissue were increased with the prolonging of the time of exposure to dry heat, while the wet/dry weight ratio of the lungs was the highest in the moderate heat-stroke group.The lung injury scores were positively correlated with the wet/dry weight ratio of the lungs, the total protein level in the BALF, and the content of MDA in the lung tissue (P<0.01). Conclusion Heatstroke in the xerothermic environment of desert can cause lung injury in rats, which deteriorates with the prolonging of the time of exposure to dry heat.As for the management of such heatstroke, more attention should be paid to the protection against lung injury.
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