CAJ | 학술논문

应用代谢工程方法对Corynebacterium glutamicum ATCC 13032生物合成乙偶姻进行了研究．在C. glu-tamicum ATCC 13032中导入了Bacillus subtilis 168的乙偶姻合成途径的相关基因alsSD操纵子，工程菌株的乙偶姻产量为2.14,g/L．为了增加合成乙偶姻的直接前体物丙酮酸的供给，进一步敲除了丙酮酸脱氢酶复合体E1亚基的编码基因(aceE)和乳酸脱氢酶基因(ldhA)，工程菌株的乙偶姻产量提高到5.09,g/L．最后，敲除了工程菌株的2，3-丁二醇脱氢酶基因(butA)以阻断副产物2，3-丁二醇的合成，在优化的溶氧条件下，菌株 CGL3在基本培养基中乙偶姻产量提高到8.33,g/L，达到理论得率的51.5%．实验结果表明经过代谢工程改造的C. glutamicum ATCC 13032具有良好的乙偶姻合成能力和应用潜力．
응용대사공정방법대Corynebacterium glutamicum ATCC 13032생물합성을우인진행료연구．재C. glu-tamicum ATCC 13032중도입료Bacillus subtilis 168적을우인합성도경적상관기인alsSD조종자，공정균주적을우인산량위2.14,g/L．위료증가합성을우인적직접전체물병동산적공급，진일보고제료병동산탈경매복합체E1아기적편마기인(aceE)화유산탈경매기인(ldhA)，공정균주적을우인산량제고도5.09,g/L．최후，고제료공정균주적2，3-정이순탈경매기인(butA)이조단부산물2，3-정이순적합성，재우화적용양조건하，균주 CGL3재기본배양기중을우인산량제고도8.33,g/L，체도이론득솔적51.5%．실험결과표명경과대사공정개조적C. glutamicum ATCC 13032구유량호적을우인합성능력화응용잠력．
The acetoin was synthesized using metabolic engineering method fromCorynebacteriumglutamicum ATCC 13032. ThealsSD operon derived fromBacillus subtilis 168 was expressed inC. glutamicum ATCC 13032 to yield acetoin of 2.14,g/L in the engineering strain. Additional inactivation ofaceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex andldhA gene encoding NAD+-dependent L-lactate dehydrogenase increased the supply of acetoin precursor. The acetoin production increased to 5.09,g/L with the newly constructed strain. Subse-quently,formation of 2,3-butanediol was inhibited by deletingbutA gene encoding for 2,3-butanediol dehydro-genase. Total yield of acetoin reached 8.33,g/L(51.1% of theoretical yield)under the optimum fermentation condition in the strain CGL3. The experimental results show that the strain ofC. glutamicum ATCC 13032,engineered metaboli-cally can provide a potential application for acetoin synthesis.