甘肃畜牧兽医
甘肅畜牧獸醫
감숙축목수의
GANSU ANIMAL AND VETERINARY SCIENCES
2014年
11期
44-46,50
,共4页
张丽鸿%张红亮%田发益
張麗鴻%張紅亮%田髮益
장려홍%장홍량%전발익
牛多杀性巴氏杆菌%流行病学%ELISA
牛多殺性巴氏桿菌%流行病學%ELISA
우다살성파씨간균%류행병학%ELISA
pasteurella multocida of Bostaurusdomestica%epidemiology%ELISA
为了解西藏林芝地区黄牛多杀性巴氏杆菌病流行病学特点、致病机理,对该地区章麦村10户牧民养殖的黄牛血清、鼻液分泌物进行了提取,采用双抗原夹心酶联免疫法(ELISA)测定样品中多杀性巴氏杆菌抗体(PMAb)。结果表明,该地有3户牧民家黄牛血清呈多杀性巴氏杆菌抗体阳性,鼻液分泌物均呈抗体阴性。说明牛多杀性巴氏杆菌对该地区的黄牛均有不同程度的危害。
為瞭解西藏林芝地區黃牛多殺性巴氏桿菌病流行病學特點、緻病機理,對該地區章麥村10戶牧民養殖的黃牛血清、鼻液分泌物進行瞭提取,採用雙抗原夾心酶聯免疫法(ELISA)測定樣品中多殺性巴氏桿菌抗體(PMAb)。結果錶明,該地有3戶牧民傢黃牛血清呈多殺性巴氏桿菌抗體暘性,鼻液分泌物均呈抗體陰性。說明牛多殺性巴氏桿菌對該地區的黃牛均有不同程度的危害。
위료해서장림지지구황우다살성파씨간균병류행병학특점、치병궤리,대해지구장맥촌10호목민양식적황우혈청、비액분비물진행료제취,채용쌍항원협심매련면역법(ELISA)측정양품중다살성파씨간균항체(PMAb)。결과표명,해지유3호목민가황우혈청정다살성파씨간균항체양성,비액분비물균정항체음성。설명우다살성파씨간균대해지구적황우균유불동정도적위해。
In order to know the epidemiological characters and pathogenesis of pasteurella multocida of bostaurusdomestica in Nyingchi areas of Tibet,10 herdsmen’s cattles’ serum, nasal fluid of Zhangmai village were sampled by the method of double antigen sandwich ELISA to test the antibody of pasteurella multocida.The results showed that 3 herdsmen ’s cattles’ serum were positive, the nasal fluid were all negative. This demonstrated that the pasteurella multocida of bostaurusdomestica is harmful to the cattles in Nyingchi areas of Tibet.