CAJ | 학술논문

研究了鲫鱼在肝胰脏抗氧化酶活性、肝胰脏铜含量和血液红细胞微核等不同层面对铜胁迫的响应和甘草的解毒功能。试验分3组，自来水对照组、水中加硫酸铜组、加铜和甘草组，连续处理7d。结果表明，处理2、3、4d时，加铜组中鲫鱼肝脏超氧化歧化酶、过氧化物酶活性及丙二醛含量均显著高于自来水对照组，随时间延长超氧化歧化酶、过氧化物酶活性逐渐升高，而丙二醛含量却逐渐下降。加入甘草后，鲫鱼肝脏超氧化歧化酶、过氧化物酶活性及丙二醛含量均低于加铜组。处理4、7d时加铜和甘草组中鲫鱼肝脏铜含量分别为13．08 mg／kg、14．96 mg／kg ，高于加铜组的10．83 mg／kg、11．64 mg／kg。处理7 d时，加铜和甘草组中鲫鱼外周血红细胞微核率为3．17％低于加铜组的4．17％，这说明甘草具有一定的解毒功能。
연구료즉어재간이장항양화매활성、간이장동함량화혈액홍세포미핵등불동층면대동협박적향응화감초적해독공능。시험분3조，자래수대조조、수중가류산동조、가동화감초조，련속처리7d。결과표명，처리2、3、4d시，가동조중즉어간장초양화기화매、과양화물매활성급병이철함량균현저고우자래수대조조，수시간연장초양화기화매、과양화물매활성축점승고，이병이철함량각축점하강。가입감초후，즉어간장초양화기화매、과양화물매활성급병이철함량균저우가동조。처리4、7d시가동화감초조중즉어간장동함량분별위13．08 mg／kg、14．96 mg／kg ，고우가동조적10．83 mg／kg、11．64 mg／kg。처리7 d시，가동화감초조중즉어외주혈홍세포미핵솔위3．17％저우가동조적4．17％，저설명감초구유일정적해독공능。
Crucian carp (Carassius auratus) was bathed in tapwater (control group) ,water containing cop‐per and water supplemented with licorice for 7 days to evaluate detoxification function of licorice and re‐sponse of crucian carp to Cu2+ stress w ere studied via antioxidant enzyme activity ,Cu2+ content and micro‐nucleus levels in red cells . Results showed that there were significantly higher superoxide dismutase (SOD) ,and peroxidase (POD) activities and malonaldehyde (MDA) content in the fish bathed in the wa‐ter containing copper than those in the control group when the fish were bathed in the Cu2+ containing wa‐ter for 2 ,3 and 4 days .The activities of SOD and POD were increased significantly as Cu2+ exposure elasped ,but MDA content gradually decreased .The fish bathed in the licorice containing water had lower SOD ,and POD activities and MDA content in hepatopancreas than the fish in the control group .There was Cu2+ content of 13 .08 mg/kg and 14 .96 mg/kg in hepatopancreas of the fish bathed in the Cu2+ con‐taining water for 4 and 7 days ,significantly higher than those in the fish bathed in the licorice containing water (10 .83 mg/kg and 11 .64 mg/kg) .There was significantly lower micronucleus rate in the fish bathed in the licorice containing water(3 .17% ) than that in the fish bathed in the Cu2+ containing water (4 .17% ) for 7days ,indicating that licorice has a certain detoxification function .