CAJ | 학술논문

为探究不同养殖年份的鱼塘底泥中细菌多样性及演替关系，通过构建16S rRN A基因文库，比较分析养殖1年和养殖4年池塘底泥样品细菌的群落构成，分别由养殖1年和养殖4年文库获得145和172个克隆子，香农—威纳多样性指数分别为2．37和2．22，辛普森优势度指数分别为9．36和7．58，覆盖度指数分别为100％和93．3％。 Prolixibacter （34／145）、荚硫菌属（17／145）、浮霉菌属（15／145）和脱硫微菌属（12／145）为养殖1年样品优势菌群，长绳菌属（41／172）、Prolixibacter （31／172）、黄色单胞菌属（22／172）、Cetobacterium （21／172）和噬纤维菌属（15／172）为养殖4年样品优势菌群。养殖1年和养殖4年文库细菌菌群结构在种分类水平上的相似度系数为0．6171。由此可知，养殖池塘底泥中蕴含着复杂的微生物群落体系，长期的积累促使该微生物群落发生了演替。
위탐구불동양식년빈적어당저니중세균다양성급연체관계，통과구건16S rRN A기인문고，비교분석양식1년화양식4년지당저니양품세균적군락구성，분별유양식1년화양식4년문고획득145화172개극륭자，향농—위납다양성지수분별위2．37화2．22，신보삼우세도지수분별위9．36화7．58，복개도지수분별위100％화93．3％。 Prolixibacter （34／145）、협류균속（17／145）、부매균속（15／145）화탈류미균속（12／145）위양식1년양품우세균군，장승균속（41／172）、Prolixibacter （31／172）、황색단포균속（22／172）、Cetobacterium （21／172）화서섬유균속（15／172）위양식4년양품우세균군。양식1년화양식4년문고세균균군결구재충분류수평상적상사도계수위0．6171。유차가지，양식지당저니중온함착복잡적미생물군락체계，장기적적루촉사해미생물군락발생료연체。
The bacterial diversity and succession was investigated in 1 year pond‐sediment (M1) and 4 year pond‐sediment (M4) using the constructed 16S rRNA gene library .A total of 145 clones was obtained from M1 with Shannon‐waver index of 2 .37 and 172 clones from M4 with Shannon‐waver index of 2 .22 . The Simpson was found to be 9 .36 in M1 with coverage of 100% and 7 .58 in M4 with coverage of 93 .3% . The M1 were dominanted by Prolixibacter (34/145) ,Thiocapsa (17/145) , Planctomycetacia (15/145) and Desulfomicrobium (12/145) ,while Longilinea (41/172) , Prolixibacter (31/172) , Xanthomonas (22/172) ,Cetobacterium (21/172) and Cytophaga (15/172) were prevalent in M4 .The community si‐milarity coefficient between M1 and M4 was 0 .6171 at species level .The findings suggested that the fishpond was colonized a complicated bacterial community ,and the community succession associated with the culture process .