CAJ | 학술논문

采用生物测定的方法比较Prima蛋白饵剂和自行研制蛋白饵剂的引诱效果。结果表明，两者对橘小实蝇(Bactrocera dorsalis (Hendel))引诱效果相当，但水解后的同种蛋白饵剂引诱效果高于水解前。采用柱前衍生高效液相色谱法(AccQ·Tag法)测定以上两种蛋白饵剂中氨基酸的种类和含量，结果表明，这两种蛋白饵剂中均含有游离氨基酸17种，总氨基酸18种。其中Prima蛋白饵剂总氨基酸含量为52.8476 mg/mL，游离氨基酸含量为5.7364 mg/mL，多肽含量为4.71112 mg/mL；自行研制的橘小实蝇蛋白饵剂总氨基酸含量为65.6243 mg/mL，游离氨基酸含量为6.3010 mg/mL，多肽含量为59.3233 mg/mL。通过比较两者氨基酸的差别，为蛋白饵剂的工业化生产质量监控提供方法依据。
채용생물측정적방법비교Prima단백이제화자행연제단백이제적인유효과。결과표명，량자대귤소실승(Bactrocera dorsalis (Hendel))인유효과상당，단수해후적동충단백이제인유효과고우수해전。채용주전연생고효액상색보법(AccQ·Tag법)측정이상량충단백이제중안기산적충류화함량，결과표명，저량충단백이제중균함유유리안기산17충，총안기산18충。기중Prima단백이제총안기산함량위52.8476 mg/mL，유리안기산함량위5.7364 mg/mL，다태함량위4.71112 mg/mL；자행연제적귤소실승단백이제총안기산함량위65.6243 mg/mL，유리안기산함량위6.3010 mg/mL，다태함량위59.3233 mg/mL。통과비교량자안기산적차별，위단백이제적공업화생산질량감공제공방법의거。
This article mainly compares the similarities and differences between Prima produced by Malaysia Pupuk Alam company and protein bait for Bactrocera dorsalis (Hendel) developed by our research institute. The biological test was used to compare attractive effects, and High Performance Liquid Chromatography with Precolumn Derivatization (AccQ · Tag) was used to analyse the kinds and contents of amino acids. The results showed the attractive effects of Prima and protein bait were similar, and the two protein baits all contained 17 kinds of free amino acids and 18 total amino acids. Prima contained 52.847 6 mg/mL of total amino acids, 5.736 4 mg/mL of free amino acid, and 47.111 2 mg/mL of peptides. The protein bait was consisted of 65.624 3 mg of total amino acids, 6.301 0 mg of free amino acid, 59.323 3 mg of peptides in one milliliter. Besides, the attractive effect of hydrolyzed protein was stronger than before. The results can provide a basis for monitoring protein bait quality on the industrial production.