CAJ | 학술논문

从海产虾壳上分离筛选拮抗放线菌、研究其抑菌活性具有重要的实践意义。藤黄轮丝链霉菌(Streptomyces luteoverticillatus)T0907-107是分离自海产虾壳上并经初筛和鉴定的一株链霉菌，为了进一步开发和利用该菌，本研究采用极性不同的5种溶剂(丙酮、乙酸乙酯、正丁醇、乙醇和甲醇)，对该菌株的发酵液菌体活性物质进行了提取，对粗提物的理化性质和抑菌活性进行了初步研究。结果表明，其发酵液活性物质大部分存在于菌体中，采用甲醇和丙酮浸提的效果好，其抑菌圈直径分别为26.20和22.50 mm；对粗提物进行耐热性实验，结果表明，粗提物是一种能够耐60℃高温和一定酸碱的物质；粗提物对蛋白酶K稳定；菌体及粗提物对多种植物病原菌物如烟草炭疽病菌(Colletotrichum nicotianae Sacc.)、烟草赤星病菌(Alternaria alternata (Fries) Keissler)、烟草立枯丝核病菌(Rhizoctonia solani Kühn)、枇杷炭疽菌(C. gloeosporioides (Penz.) Sacc.)和白菜炭疽病菌(C. higginsianum Sacc.)有较强抑制作用。研究结果表明，甲醇和丙酮是较合适的萃取溶剂，该菌发酵液菌体粗提物极性强、稳定性好、有较广的抑菌谱，显示出好的开发前景。本研究为进一步鉴定该活性物质及开发和利用该菌提供了基础资料。
종해산하각상분리사선길항방선균、연구기억균활성구유중요적실천의의。등황륜사련매균(Streptomyces luteoverticillatus)T0907-107시분리자해산하각상병경초사화감정적일주련매균，위료진일보개발화이용해균，본연구채용겁성불동적5충용제(병동、을산을지、정정순、을순화갑순)，대해균주적발효액균체활성물질진행료제취，대조제물적이화성질화억균활성진행료초보연구。결과표명，기발효액활성물질대부분존재우균체중，채용갑순화병동침제적효과호，기억균권직경분별위26.20화22.50 mm；대조제물진행내열성실험，결과표명，조제물시일충능구내60℃고온화일정산감적물질；조제물대단백매K은정；균체급조제물대다충식물병원균물여연초탄저병균(Colletotrichum nicotianae Sacc.)、연초적성병균(Alternaria alternata (Fries) Keissler)、연초립고사핵병균(Rhizoctonia solani Kühn)、비파탄저균(C. gloeosporioides (Penz.) Sacc.)화백채탄저병균(C. higginsianum Sacc.)유교강억제작용。연구결과표명，갑순화병동시교합괄적췌취용제，해균발효액균체조제물겁성강、은정성호、유교엄적억균보，현시출호적개발전경。본연구위진일보감정해활성물질급개발화이용해균제공료기출자료。
It has important practical significance to isolate and screen antagonistic actinomycetes from marine shrimp shell and study their antimicrobial activity. Streptomyces luteoverticillatus strain T0907-107 was isolated and identified from shrimp shell. In order to furtherly exploit and utilize the bacteria, antimicrobial activity substance was extracted from the thalli of strain T0907-107 which was collected from fermentation broth by 5 different solvents, acetone, ethyl acetate, n- butanol, ethanol and methanol. Antimicrobial activity and stability of the antimicrobial metabolites produced from strain T0907-107 were carried out. The results showed that acetone and methanol were the best solvents for the crude extract, and the inhibition zones were 26.20 and 22.50 mm, respectively. After protease K treatment, the crude extract still had inhibitory effects to the fungus. The crude extracted substance was material of thermostability (60℃) and had a good performance of acid and alkali resistance. The thalli of the strain and the crude extracted substance presented a strong antifungal activity against half of tested plant pathogenic fungi such as Colletotrichum nicotianae Sacc., Alternaria alternata(Fries)Keissler, Rhizoctonia solani Kühn, C. gloeosporioides (Penz.) Sacc. and C. higginsianum Sacc..The results showed that the acetone and methanol were good extraction solvents, the crude extracted substance had strong polarity and a strange of thermostability, acid and alkali resistance, which indicated that the bacteria had good development prospect. This study provides basic data for further identification of the active material and the exploitation and utilization of the strain T0907-107.