CAJ | 학술논문

陆地棉皱缩叶突变体是本项目组自主发现的一种自然突变体。前期对其表型性状的观察发现该突变体的农艺性状有别于前人已报道的皱叶突变体；通过陆陆杂交世代群体对其遗传规律的研究表明，皱缩叶突变性状是受一对完全隐性基因wr3控制的质量性状。文章以皱叶突变自交系L037和海7124为亲本配置组合得到的海陆杂交F2为作图群体，利用本实验室遴选的平均覆盖棉花26对染色体上的234对SSR引物(每染色体相对等距离分布9对引物)，通过对双亲以及近等基因池的筛选，共得到12对多态性引物。用这些引物检测 F2作图群体每个单株的标记基因型，经Join Map 4.0分析显示，位于Chr.21的引物BNL3279与目的基因连锁，二者间的遗传距离为28.7 cM。随后对Chr.21上的其他引物进一步筛选，共得到16对与目的基因连锁的标记，其中与目的基因距离最近的标记NAU3740的遗传距离为4.8 cM，另一侧标记cgr5428的遗传距离为10.4 cM。由此推定，皱缩叶突变体基因wr3在棉花第21染色体上。
륙지면추축협돌변체시본항목조자주발현적일충자연돌변체。전기대기표형성상적관찰발현해돌변체적농예성상유별우전인이보도적추협돌변체；통과륙륙잡교세대군체대기유전규률적연구표명，추축협돌변성상시수일대완전은성기인wr3공제적질량성상。문장이추협돌변자교계L037화해7124위친본배치조합득도적해륙잡교F2위작도군체，이용본실험실린선적평균복개면화26대염색체상적234대SSR인물(매염색체상대등거리분포9대인물)，통과대쌍친이급근등기인지적사선，공득도12대다태성인물。용저사인물검측 F2작도군체매개단주적표기기인형，경Join Map 4.0분석현시，위우Chr.21적인물BNL3279여목적기인련쇄，이자간적유전거리위28.7 cM。수후대Chr.21상적기타인물진일보사선，공득도16대여목적기인련쇄적표기，기중여목적기인거리최근적표기NAU3740적유전거리위4.8 cM，령일측표기cgr5428적유전거리위10.4 cM。유차추정，추축협돌변체기인wr3재면화제21염색체상。
The phenotype of a wrinkled leaf mutant in upland cotton newly found was different from other analogous mutants reported previously. Genetic analysis indicates that the mutation is controlled by a single recessive gene wr3. In this paper, an F2 mapping population derived from the mutation inbred line L037 crossing with Hai7124 (Gossy-pium babardense) was used. A total of 12 pairs of polymorphic primers were selected from 234 pairs of SSR primers obtained before, which covered 26 pairs of cotton chromosomes at regular intervals. Genetic mapping of the F2 pop-ulation showed that gene wr3 was linked to the primer BNL3279 on Chr.21 with the distance of 28.7 cM. Further screening of other primers on Chr.21 showed that gene wr3 was flanked by the primers NAU3740 and cgr5428, with genetic distance of 4.8 cM and 10.4 cM, respectively.