遗传
遺傳
유전
HEREDITAS(BEIJING)
2014年
12期
1226-1234
,共9页
樊春燕%魏强%郝志强%李广林
樊春燕%魏彊%郝誌彊%李廣林
번춘연%위강%학지강%리엄림
微小RNAs%基因间长链非编码RNAs%降解组%靶标
微小RNAs%基因間長鏈非編碼RNAs%降解組%靶標
미소RNAs%기인간장련비편마RNAs%강해조%파표
microRNAs (miRNAs)%long intergenic non-coding RNAs (lincRNAs)%degradome data%targets
基因间长链非编码RNAs(Long intergenic non-coding RNAs, lincRNAs)是位于蛋白编码基因之间的长度超过200 nt的非编码RNAs，在动物中参与细胞周期调控、免疫监视、胚胎干细胞分化等多种生物学过程，但是lincRNAs在大多数植物中的功能尚不清楚。MicroRNAs(miRNAs)是真核生物中一类在转录水平和转录后水平介导基因沉默的21 nt左右的内源性单链小非编码RNAs分子，通过序列互补的方式调控靶标基因的表达。目前miRNAs的靶标研究主要集中于编码蛋白的基因，而对于靶标为非编码RNAs的研究较少，尤其在植物中的研究更为少见。为了系统挖掘植物中lincRNAs的功能，文章整合miRNAs数据、cDNAs数据和降解组数据，利用生物信息学方法找到拟南芥(Arabidopsis thaliana)337个成熟miRNAs在2708个lincRNAs上的可能结合位点，构建了miRNAs-mRNAs-lincRNAs调控网络，并根据竞争性内源(ceRNA)假说预测lincRNAs的功能，为进一步阐明植物中miRNAs对lincRNAs的调控机制以及lincRNAs的功能奠定了基础。
基因間長鏈非編碼RNAs(Long intergenic non-coding RNAs, lincRNAs)是位于蛋白編碼基因之間的長度超過200 nt的非編碼RNAs，在動物中參與細胞週期調控、免疫鑑視、胚胎榦細胞分化等多種生物學過程，但是lincRNAs在大多數植物中的功能尚不清楚。MicroRNAs(miRNAs)是真覈生物中一類在轉錄水平和轉錄後水平介導基因沉默的21 nt左右的內源性單鏈小非編碼RNAs分子，通過序列互補的方式調控靶標基因的錶達。目前miRNAs的靶標研究主要集中于編碼蛋白的基因，而對于靶標為非編碼RNAs的研究較少，尤其在植物中的研究更為少見。為瞭繫統挖掘植物中lincRNAs的功能，文章整閤miRNAs數據、cDNAs數據和降解組數據，利用生物信息學方法找到擬南芥(Arabidopsis thaliana)337箇成熟miRNAs在2708箇lincRNAs上的可能結閤位點，構建瞭miRNAs-mRNAs-lincRNAs調控網絡，併根據競爭性內源(ceRNA)假說預測lincRNAs的功能，為進一步闡明植物中miRNAs對lincRNAs的調控機製以及lincRNAs的功能奠定瞭基礎。
기인간장련비편마RNAs(Long intergenic non-coding RNAs, lincRNAs)시위우단백편마기인지간적장도초과200 nt적비편마RNAs，재동물중삼여세포주기조공、면역감시、배태간세포분화등다충생물학과정，단시lincRNAs재대다수식물중적공능상불청초。MicroRNAs(miRNAs)시진핵생물중일류재전록수평화전록후수평개도기인침묵적21 nt좌우적내원성단련소비편마RNAs분자，통과서렬호보적방식조공파표기인적표체。목전miRNAs적파표연구주요집중우편마단백적기인，이대우파표위비편마RNAs적연구교소，우기재식물중적연구경위소견。위료계통알굴식물중lincRNAs적공능，문장정합miRNAs수거、cDNAs수거화강해조수거，이용생물신식학방법조도의남개(Arabidopsis thaliana)337개성숙miRNAs재2708개lincRNAs상적가능결합위점，구건료miRNAs-mRNAs-lincRNAs조공망락，병근거경쟁성내원(ceRNA)가설예측lincRNAs적공능，위진일보천명식물중miRNAs대lincRNAs적조공궤제이급lincRNAs적공능전정료기출。
Long intergenic non-coding RNAs (lincRNAs) located between protein-coding genes are non-coding RNAs longer than 200 nucleotides in length. LincRNAs are involved in a variety of biological processes such as cell cycle regulation, immune surveillance and embryonic stem cells differentiation in animals;however, the func-tion of lincRNAs in plants is largely unclear. MicroRNAs (miRNAs) are a class of endogenous, single-stranded, non-coding small (~21 nt) RNAs, which can regulate gene expression at transcriptional or post-transcriptional level in eukaryotes by means of sequence complementation. Now, intensive studies on protein-coding genes targeted by miRNAs have been carried out, but the research on non-coding RNAs targeted by miRNAs is seldom explored, espe-cially in plants. In order to uncover the potential function of lincRNAs, the data including miRNAs, cDNAs and de-gradomes were firstly collected and integrated, followed by bioinformatics methods to predict the potential binding sites of 337 mature miRNAs at 2708 lincRNAs in Arabidopsis thaliana. The regulatory networks of miR-NAs-mRNAs-lincRNAs were constructed and the function of lincRNAs was predicted according to the competing endogenous RNA (ceRNA) hypothesis. This study may lay a solid foundation for elucidating the regulatory mechan-ism of miRNAs on lincRNAs as well as the function of lincRNAs in plants.
