国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2014年
12期
2143-2146
,共4页
齐艳秀%符俊达%王玉清%王冬兰
齊豔秀%符俊達%王玉清%王鼕蘭
제염수%부준체%왕옥청%왕동란
糖尿病%糖尿病视网膜病变%丙二醛%谷胱甘肽过氧化物酶%丙酮酸
糖尿病%糖尿病視網膜病變%丙二醛%穀胱甘肽過氧化物酶%丙酮痠
당뇨병%당뇨병시망막병변%병이철%곡광감태과양화물매%병동산
diabetes mellitus%diabetic retinopathy%malonie dialdehyde%glutathione peroxidase%pyruvate
目的：研究糖尿病大鼠视网膜病变过程中视网膜组织学和氧化应激的变化,以及丙酮酸的对抗作用。<br> 方法：将80只Wistar大鼠分成3组：对照组(20只),模型组(30只),治疗组(30只)。模型组和治疗组用STZ诱导糖尿病,治疗组在大鼠饲料和饮水中添加2%丙酮酸。观察大鼠的血糖、体质量变化,并在造模后12 wk观察3组大鼠视网膜组织中GSH-Px、MDA和Na+-K+-ATP酶水平及其超微结构改变。<br> 结果：模型组和对照组相比,体质量显著下降,视网膜中GSH-Px和ATP酶活性显著下降,MDA水平显著升高,视网膜超微结构有显著改变；治疗组和模型组相比,血糖没有显著改变,视网膜组织中 GSH-Px 和 ATP 水平升高, MDA水平下降,视网膜超微结构病变相对较轻。<br> 结论：丙酮酸可以减轻氧化应激反应,改善视网膜的能量代谢,延缓视网膜病变的发展。
目的：研究糖尿病大鼠視網膜病變過程中視網膜組織學和氧化應激的變化,以及丙酮痠的對抗作用。<br> 方法：將80隻Wistar大鼠分成3組：對照組(20隻),模型組(30隻),治療組(30隻)。模型組和治療組用STZ誘導糖尿病,治療組在大鼠飼料和飲水中添加2%丙酮痠。觀察大鼠的血糖、體質量變化,併在造模後12 wk觀察3組大鼠視網膜組織中GSH-Px、MDA和Na+-K+-ATP酶水平及其超微結構改變。<br> 結果：模型組和對照組相比,體質量顯著下降,視網膜中GSH-Px和ATP酶活性顯著下降,MDA水平顯著升高,視網膜超微結構有顯著改變；治療組和模型組相比,血糖沒有顯著改變,視網膜組織中 GSH-Px 和 ATP 水平升高, MDA水平下降,視網膜超微結構病變相對較輕。<br> 結論：丙酮痠可以減輕氧化應激反應,改善視網膜的能量代謝,延緩視網膜病變的髮展。
목적：연구당뇨병대서시망막병변과정중시망막조직학화양화응격적변화,이급병동산적대항작용。<br> 방법：장80지Wistar대서분성3조：대조조(20지),모형조(30지),치료조(30지)。모형조화치료조용STZ유도당뇨병,치료조재대서사료화음수중첨가2%병동산。관찰대서적혈당、체질량변화,병재조모후12 wk관찰3조대서시망막조직중GSH-Px、MDA화Na+-K+-ATP매수평급기초미결구개변。<br> 결과：모형조화대조조상비,체질량현저하강,시망막중GSH-Px화ATP매활성현저하강,MDA수평현저승고,시망막초미결구유현저개변；치료조화모형조상비,혈당몰유현저개변,시망막조직중 GSH-Px 화 ATP 수평승고, MDA수평하강,시망막초미결구병변상대교경。<br> 결론：병동산가이감경양화응격반응,개선시망막적능량대사,연완시망막병변적발전。
AlM:To investigate the changes of retinal histology and oxidative stress in diabetic retinopathy and its reversal by pyruvate in diabetic rats. <br> METHODS: Eighty Wistar rats were divided into 3 groups:control group ( 20 rats ) , model group ( 30 rats ) and treatment group ( 30 rats ) . After streptozotocin ( STZ) induced diabetes mellitus in the model group and the treatment group, the treatment group received 2%pyruvate in diet and drinking. The changes of body weight and blood glucose were observed and the changes of glutathione peroxidase ( GSH-PX ) , malonie dialdehyde ( MDA) , and Na+-K+-ATPase levels of retinal tissue and retinal ultrastructure were investigated in three groups at 12wk after occurrence of diabetes. <br> RESULTS: Compared with control group, the body weight of the model group were significantly decreased, the activities of GSH-PX and ATP in the retina of diabetic rats were significantly lower, the MDA was signigicantly higher and significant changes occurred in retinal ultrastructure. Compared with model group, the blood glucose of the treatment group had no significant changes. However, the activities of GSH and ATP in the retina of diabetic rats were higher, the MDA was lower and the retinal ultrastructure was comparatively mild. <br> CONCLUSlON:Pyruvate can alleviate oxidatie stress reaction, improve the energy metabolism of retina, and delay the development of retinopathy.
