CAJ | 학술논문

目的：探讨缺氧诱导因子-1α( hypoxia-inducible factor-1α,HIF-1α)及促红细胞生成素( erythropoietin,EPO)在碱烧伤大鼠角膜组织中的表达,评估其在角膜新生血管( corneal neovascularization,CNV)发生发展中的作用。 　　方法：健康3月龄SD大鼠,随机分为实验组和对照组,碱烧伤法建立CNV模型,分别于造模成功后1,3,5,7,14 d测量CNV的长度并计算面积,同时取角膜组织以免疫组织化学检测HIF-1α及EPO的表达部位,并以RT-PCR法检测其mRNA的表达情况,实验数据采用SPSS 20.0处理。 　　结果：碱烧伤后1,3,5,7,14d,CNV的面积随时间逐渐增加,7 d生长最为旺盛,14 d后生长减慢。免疫组织化学提示：正常角膜各层未见HIF-1α的表达,可见微量EPO,碱烧伤后1 d时HIF-1α及EPO免疫活性增强,主要表达于角膜上皮及内皮层。 RT-PCR 结果显示 HIF-1α及EPO mRNA的表达在正常大鼠角膜组织中表达极少,在角膜碱烧伤后3 d表达增强,7 d达到高峰,14 d后明显下降；不同时间点组间比较存在统计学差异(P<0.05)。 　　结论：HIF-1α和EPO与CNV的形成密切相关。
목적：탐토결양유도인자-1α( hypoxia-inducible factor-1α,HIF-1α)급촉홍세포생성소( erythropoietin,EPO)재감소상대서각막조직중적표체,평고기재각막신생혈관( corneal neovascularization,CNV)발생발전중적작용。 　　방법：건강3월령SD대서,수궤분위실험조화대조조,감소상법건립CNV모형,분별우조모성공후1,3,5,7,14 d측량CNV적장도병계산면적,동시취각막조직이면역조직화학검측HIF-1α급EPO적표체부위,병이RT-PCR법검측기mRNA적표체정황,실험수거채용SPSS 20.0처리。 　　결과：감소상후1,3,5,7,14d,CNV적면적수시간축점증가,7 d생장최위왕성,14 d후생장감만。면역조직화학제시：정상각막각층미견HIF-1α적표체,가견미량EPO,감소상후1 d시HIF-1α급EPO면역활성증강,주요표체우각막상피급내피층。 RT-PCR 결과현시 HIF-1α급EPO mRNA적표체재정상대서각막조직중표체겁소,재각막감소상후3 d표체증강,7 d체도고봉,14 d후명현하강；불동시간점조간비교존재통계학차이(P<0.05)。 　　결론：HIF-1α화EPO여CNV적형성밀절상관。
AlM: To describe the expression of hypoxia-inducible factor-1α ( HlF-1α) and erythropoietin ( EPO ) in rats' corneal and evaluate its potential effect on corneal neovascularization ( CNV) growth. METHODS:The young SD rats (3mo) was chosen and randomly divided into 2 groups, which were experimental group and normal control group. CNV model was established by alkali burn, and the length and area of CNV was observed everyday after operation by slit lamp. After that, the expression of HlF-1α and EPO was measured by SABC and RT-PCR methods at 1, 3, 5, 7, and 14d after alkali burn. The data was analyzed by SPSS 20. 0. RESULTS:The area of CNV was increasing at 1, 3, 5, 7, and 14d after alkali burn, and the peak point appear at 7d. The growth speed was decreased after 14d. SABC method told us that no HlF-1αand very tiny amount EPO was detected at normal rats' corneal. The expression of the two factors increased at 1d after alkali burn in corneal epithelium and endoderm. The results of RT - PCR showed that a few amounts of HlF-1α and EPO mRNA were detected at normal group. The expression of the two factors was increased at 3d after alkali burn, and the peak value was found at 7d, however, it was decreased at 14d. Statistical difference was found at different time (P<0. 05). CONCLUSlON: HlF- 1α and EPO is closely related to CNV.