CAJ | 학술논문

目的：研究高氧诱导的视网膜新生血管模型鼠中转录因子Islet-1的表达差异。 　　方法：采用高氧诱导的方法制作鼠视网膜新生血管模型,运用荧光造影视网膜铺片及视网膜切片苏木精-伊红染色观察视网膜新生血管的形态。于小鼠出生后第7,12,14,17,26 d取视网膜组织,采用Real-time PCR及Western blot技术测定视网膜组织中Islet-1的表达水平。 　　结果：模型组视网膜铺片及组织切片可见大量视网膜新生血管形成。小鼠出生后第7d,模型组与正常组视网膜组织中Islet-1表达水平无明显差异；小鼠出生后第12~14d,模型组视网膜组织中Islet-1表达水平明显上调；出生后17d,模型组视网膜组织中Islet-1表达水平仍高于正常组；出生后26d,随着视网膜新生血管消退,视网膜组织中Islet-1表达水平降至正常水平。 　　结论：模型鼠视网膜新生血管发生过程中,持续缺氧的视网膜组织通过增加转录因子Islet-1的表达,从而诱导视网膜新生血管的发生。
목적：연구고양유도적시망막신생혈관모형서중전록인자Islet-1적표체차이。 　　방법：채용고양유도적방법제작서시망막신생혈관모형,운용형광조영시망막포편급시망막절편소목정-이홍염색관찰시망막신생혈관적형태。우소서출생후제7,12,14,17,26 d취시망막조직,채용Real-time PCR급Western blot기술측정시망막조직중Islet-1적표체수평。 　　결과：모형조시망막포편급조직절편가견대량시망막신생혈관형성。소서출생후제7d,모형조여정상조시망막조직중Islet-1표체수평무명현차이；소서출생후제12~14d,모형조시망막조직중Islet-1표체수평명현상조；출생후17d,모형조시망막조직중Islet-1표체수평잉고우정상조；출생후26d,수착시망막신생혈관소퇴,시망막조직중Islet-1표체수평강지정상수평。 　　결론：모형서시망막신생혈관발생과정중,지속결양적시망막조직통과증가전록인자Islet-1적표체,종이유도시망막신생혈관적발생。
AlM:To evaluate the expression of transcriptional factor lslet-1 in retina in experimental retinal neovascularization induced by oxygen. METHODS: The murine retinal neovascularization were induced by hyperoxia exposure. The morphological observation of retinal neovascularization was performed using angiography by fluorescein dextran injection under the fluorescence microscope, and the new blood vessels were quantified after 5d in room air (17-day-old) by counting the vascular epithelial cell nuclei protruding into viteous cavity using HE stain. Realtime PCR and Western blot were used to examine retinal lslet-1 level in postnatal 7,12, 14,17 and 26d respectively. RESULTS: A lots of new blood vessels were demonstrated in the mouse retina in hyperoxic group by fluorescein angiography and histological method. Moreover, no significant difference was found in retinal lslet-1 level in postnatal 7d between hyperoxic group and control group, but was significantly higher in postnatal 12, 14 and 17d mice compared with control mice. However, mice at postnatal 26d, expression of lslet-1 in retina decreased to normal level. CONCLUSlON: ln processing mouse model of retinal neovascularization, sustained hypoxia retinal tissue induce retinal neovascularization by increas the expression of transcription factor lslet-1.