CAJ | 학술논문

目的：评估CPS4显色培养基在分离鉴定泌尿道病原菌的诊断价值。方法收集2013年1～12月武汉大学中南医院临床科室送检的中段尿细菌培养标本1074份，以CPS4显色培养基与传统方法分别进行分离鉴定，并比较鉴定结果。结果传统方法分离出病原菌464株，其中大肠埃希菌229株（占49．4％），其次是肠球菌98株（占21．1％），检出复合菌株29例；用CPS4显色培养基分离出病原菌466株，检出复合菌株33例，与传统方法的分离培养性能基本一致，直接鉴定大肠埃希菌、肠球菌、变形杆菌及克雷伯菌属－肠杆菌属－沙雷菌属－枸橼酸杆菌属群（KESC群）的符合率达97％以上，且直接鉴定的细菌数占泌尿系统病原菌85．3％。结论 CPS4显色培养基能简便、快速地分离鉴定临床常见的泌尿道病原菌，且混合菌感染的分离鉴定更优，有利于泌尿道感染的快速诊断。
목적：평고CPS4현색배양기재분리감정비뇨도병원균적진단개치。방법수집2013년1～12월무한대학중남의원림상과실송검적중단뇨세균배양표본1074빈，이CPS4현색배양기여전통방법분별진행분리감정，병비교감정결과。결과전통방법분리출병원균464주，기중대장애희균229주（점49．4％），기차시장구균98주（점21．1％），검출복합균주29례；용CPS4현색배양기분리출병원균466주，검출복합균주33례，여전통방법적분리배양성능기본일치，직접감정대장애희균、장구균、변형간균급극뢰백균속－장간균속－사뢰균속－구연산간균속군（KESC군）적부합솔체97％이상，차직접감정적세균수점비뇨계통병원균85．3％。결론 CPS4현색배양기능간편、쾌속지분리감정림상상견적비뇨도병원균，차혼합균감염적분리감정경우，유리우비뇨도감염적쾌속진단。
Objective To evaluate the diagnostic value of CPS4 chromogenic culture media in isolation and i‐dentification of urinary pathogen .Methods 1 074 Urine samples were collected and cultured using CPS4 chromogenic and traditional culture media simultaneously ,and the identification results were compared .Results 464 strains of pathogens including 29 compound stains were identified with traditional culture media .The principal pathogens were identified as Escherichia coli(229 ,49 .4% )and enterococcus(98 ,21 .1% ) .Meanwhile ,466 strains of pathogens inclu‐ding 33 compound stains were identified with CPS4 chromogenic culture media .By comparison ,the performance of CPS4 chromogenic and traditional culture media was basically identical .The accuracy of CPS4 chromogenic culture media was 97% in identifying Escherichia coli ,Enterococcus ,Proteus and the Klebsiella ,Enterobacter ,Serratia and Citrobacter group(KESC) .85 .3% of urinary pathogens can be directly identified with color change by CPS4 chromo‐genic culture media .Conclusion The common urinary tract pathogens can be identified by CPS4 chromogenic culture media fast and conveniently ,especially for multiple infectious samples ,which could be worthy for rapid diagnosis of urinary infection .