CAJ | 학술논문

目的：探讨人乳头瘤病毒（HPV）E6／E7 mRNA水平在不同程度宫颈病变中的应用价值。方法选取2011年4月至2013年4月广东省中医院妇科门诊收治的HPV感染患者430例，将HPV‐DNA阳性标本根据病理资料分为炎症组、CINⅠ组、CIN Ⅱ组、CIN Ⅲ组和宫颈癌组。用反转录聚合酶链反应（RT‐PCR）检测各组标本的HPV E6／E7 mRNA水平，比较不同宫颈病变组间HPV‐DNA病毒载量及 HPV E6／E7 mRNA阳性检出率的差异情况。结果随着宫颈病变程度增加，HPV‐DNA病毒载量未呈现逐级递增的趋势，各病变组间的病毒载量两两比较差异无统计学意义（P＞0．05）；而HPV E6／E7 mRNA阳性检出率则逐级递增，除炎症组与CINⅠ组的检出率比较差异无统计学意义（P＞0．05）之外，其余各组之间的检出率两两比较差异均有统计学意义（P＜0．05）。结论 HPV E6／E7 mRNA与宫颈病变程度有高度的相关性，可作为宫颈疾病筛查的重要检测指标。
목적：탐토인유두류병독（HPV）E6／E7 mRNA수평재불동정도궁경병변중적응용개치。방법선취2011년4월지2013년4월광동성중의원부과문진수치적HPV감염환자430례，장HPV‐DNA양성표본근거병리자료분위염증조、CINⅠ조、CIN Ⅱ조、CIN Ⅲ조화궁경암조。용반전록취합매련반응（RT‐PCR）검측각조표본적HPV E6／E7 mRNA수평，비교불동궁경병변조간HPV‐DNA병독재량급 HPV E6／E7 mRNA양성검출솔적차이정황。결과수착궁경병변정도증가，HPV‐DNA병독재량미정현축급체증적추세，각병변조간적병독재량량량비교차이무통계학의의（P＞0．05）；이HPV E6／E7 mRNA양성검출솔칙축급체증，제염증조여CINⅠ조적검출솔비교차이무통계학의의（P＞0．05）지외，기여각조지간적검출솔량량비교차이균유통계학의의（P＜0．05）。결론 HPV E6／E7 mRNA여궁경병변정도유고도적상관성，가작위궁경질병사사적중요검측지표。
Objective To investigate the diagnostic value of human papilloma virus (HPV) E6/E7 mRNA in different degrees of cervical lesions .Methods A total of 430 patients with HPV infection ,received by the Outpatient Department of the Traditional Chinese Medicine Hospital of Guangdong Province ,were enrolled and divided into in‐flammation ,cervical epithelial atypical hyperplasia(CIN) Ⅰ ,CIN Ⅱ ,CIN Ⅲ and cervical cancer groups according to pathological data .The reverse transcription polymerase chain reaction (RT‐PCR) method was used to detect the HPV‐DNA viral load and HPV E6/E7 mRNA level .And the HPV‐DNA viral load and positive detectable rathe of HPV E6/E7 mRNA were compared among the different degrees of cervical lesions groups .Results The HPV DNA viral load was not increased with the cervical lesion degree ,and there was no significant differences between any two groups(P>0 .05) .While the positive detectable rate of HPV E6/E7 mRNA was increased with the cervical lesion degree ,and there were significant differences between any two groups(P<0 .05) ,except the comparation between the inflammation and CIN Ⅰ group .Conclusion The positive detectable rate of HPV E6/E7 mRNA might be highly correlated with the degrees of cervical lesions ,and could be an significant maker for the cervical disease screening .