CAJ | 학술논문

目的：探讨镁离子在高磷诱导慢性肾衰竭大鼠模型血管钙化中的作用及其机制。方法将32只雄性SD大鼠采用随机数字表法均分为对照组（ A组）、慢性肾衰竭血管钙化组（ B1组）、低镁组（ B2组）、高镁组（ B3组）。用硫酸腺嘌呤加高磷饮食复制慢性肾衰竭大鼠血管钙化模型。造模成功后测量4组大鼠主动脉脉搏波速率（PWV），反转录聚合酶链反应（RT-PCR）方法检测主动脉血管钙化标志分子核心结合因子α1（Cbfα1）mRNA的表达情况，von Kossa染色及邻甲酚酞络合酮比色法测定钙水平。结果 B1组血肌酐、尿素氮、血磷水平高于A组（ t=32.284、22.010、26.000，P<0.05）。4组血镁水平比较，差异有统计学意义（F=7.745，P<0.05）；B3组血镁水平高于B2组（q=2.897，P<0.05）。4组主动脉钙水平比较，差异有统计学意义（F=6.824，P<0.05）；B2组主动脉钙水平高于B1组（q=13.192，P<0.05）；B1组主动脉钙水平高于A组（q=21.925，P<0.05）；B3组主动脉钙水平低于B1组和B2组（q=9.333、21.782，P<0.05）。4组胸主动脉PWV值比较，差异有统计学意义（F=6.454，P<0.05）；B2组PWV值高于B1组（q=13.492，P<0.05）；B1组PWV值高于A组（q=14.311，P<0.05）；B3组PWV值低于B1组和B2组（q=5.333、16.865，P<0.05）。4组胸主动脉Cbfα1 mRNA表达量比较，差异有统计学意义（F=3.212，P<0.05）；B1和B2组Cbfα1 mRNA表达量高于A组（q =7.333、13.565，P <0.05）；B3组 Cbfα1 mRNA表达量低于B1组和B2组（q=10.198、13.858，P<0.05）；B2组Cbfα1 mRNA表达量高于B1组（q=12.279， P<0.05）。结论镁离子能够抑制高磷诱导的慢性肾衰竭大鼠血管钙化，改善血管弹性功能；其可能机制之一是镁离子通过抑制高磷诱导血管平滑肌细胞表型转化实现。
목적：탐토미리자재고린유도만성신쇠갈대서모형혈관개화중적작용급기궤제。방법장32지웅성SD대서채용수궤수자표법균분위대조조（ A조）、만성신쇠갈혈관개화조（ B1조）、저미조（ B2조）、고미조（ B3조）。용류산선표령가고린음식복제만성신쇠갈대서혈관개화모형。조모성공후측량4조대서주동맥맥박파속솔（PWV），반전록취합매련반응（RT-PCR）방법검측주동맥혈관개화표지분자핵심결합인자α1（Cbfα1）mRNA적표체정황，von Kossa염색급린갑분태락합동비색법측정개수평。결과 B1조혈기항、뇨소담、혈린수평고우A조（ t=32.284、22.010、26.000，P<0.05）。4조혈미수평비교，차이유통계학의의（F=7.745，P<0.05）；B3조혈미수평고우B2조（q=2.897，P<0.05）。4조주동맥개수평비교，차이유통계학의의（F=6.824，P<0.05）；B2조주동맥개수평고우B1조（q=13.192，P<0.05）；B1조주동맥개수평고우A조（q=21.925，P<0.05）；B3조주동맥개수평저우B1조화B2조（q=9.333、21.782，P<0.05）。4조흉주동맥PWV치비교，차이유통계학의의（F=6.454，P<0.05）；B2조PWV치고우B1조（q=13.492，P<0.05）；B1조PWV치고우A조（q=14.311，P<0.05）；B3조PWV치저우B1조화B2조（q=5.333、16.865，P<0.05）。4조흉주동맥Cbfα1 mRNA표체량비교，차이유통계학의의（F=3.212，P<0.05）；B1화B2조Cbfα1 mRNA표체량고우A조（q =7.333、13.565，P <0.05）；B3조 Cbfα1 mRNA표체량저우B1조화B2조（q=10.198、13.858，P<0.05）；B2조Cbfα1 mRNA표체량고우B1조（q=12.279， P<0.05）。결론미리자능구억제고린유도적만성신쇠갈대서혈관개화，개선혈관탄성공능；기가능궤제지일시미리자통과억제고린유도혈관평활기세포표형전화실현。
Objective To investigate the effect of magnesium ions on phosphorus-induced thoracic calcification in chronic renal failure rats and its mechanism. Methods 32 male SD rats were divided into four groups by random number table method:normal control group ( A group ), chronic kidney failure complicated with vascular calcification group ( B1 group ), low magnesium group(B2 group)and high magnesium group(B3 group),8 in each group. The rat models of chronic kidney failure complicated with vascular calcification were established by adenine sulphate and high phosphorus diets. The aortic pulse wave velocity(PWV)was measured after the rat model was established,the expression of core binding factor alphα1(Cbfα1) mRNA were determined by RT-PCR. The calcium levels of aorta were determined by von Kossa staining and cresolphthalein o-complexone colorimetry. Results The serum levels of creatinine,urea nitrogen and phosphorus among rats in B1 group were sig-nificantly higher than those in A group(t=32. 284,22. 010,26. 000,P<0. 05). There were significant differences in serum magnesium level among four groups(F=7. 745,P<0. 05). The serum magnesium levels among rats in B3 group were signifi-cantly higher than those in B2 group(q=2. 897,P<0. 05). There were significant differences in aortic calcium level among four groups(F=6. 824,P<0. 05). The aortic calcium levels among rats in B2 group were significantly higher than those in B1 group(q=13. 192,P<0. 05). The aortic calcium levels among rats in B1 group were significantly higher than those in A group (q=21. 925,P<0. 05). The aortic calcium levels among rats in B3 group were significantly lower than those in B1 and B2 groups,respectively(q=9. 333,21. 782,P<0. 05). There were significant differences in thoracic aortic PWV value among four groups(F=6. 454,P<0. 05). The aortic PWV values among rats in B2 group were significantly higher than those in B1 group(q=13. 492,P<0. 05). The aortic PWV values among rats in B1 group were significantly higher than those in A group (q=14. 311,P<0. 05). The aortic PWV values among rats in B3 group were significantly lower than those in B1 group and B2 group,respectively(q=5. 333,16. 865,P<0. 05). There were significant differences in aortic calcium Cbfα1 m RNA ex-pression level among four groups(F=3. 212,P<0. 05). The Cbfα1 mRNA expression levels in B1 group and B2 group were significantly higher than those in A group,respectively(q=7. 333,13. 565,P<0. 05). The Cbfα1 mRNA expression levels in B3 group were significantly lower than those in B1 group and B2 group,respectively(q=10. 198,13. 858,P<0. 05). The Cbfα1 mRNA expression levels in B2 group were significantly higher than those in B1 group(q=12. 279,P<0. 05). Conclu-sion Magnesium iron can inhibit thoracic calcification in rats with phosphorus-induced chronic renal failure,thus improve ves-sel elastic function. Magnesium ions can inhibit the phosphorus-induced phenotypic transformation of VSMCs,which is maybe one of the possible mechanisms.