中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2014年
36期
4315-4318
,共4页
蔡伟%徐积兄%张微%朱伟锋%朱凌燕%刘盈%肖钧仁%刘建英
蔡偉%徐積兄%張微%硃偉鋒%硃凌燕%劉盈%肖鈞仁%劉建英
채위%서적형%장미%주위봉%주릉연%류영%초균인%류건영
糖基化终产物,高级%基因%多态性,单核苷酸%葡糖耐量试验
糖基化終產物,高級%基因%多態性,單覈苷痠%葡糖耐量試驗
당기화종산물,고급%기인%다태성,단핵감산%포당내량시험
Glycosylation end products,advanced%Genes%Polymorphism,single nucleotide%Glucose tolerance test
目的:探讨中国汉族人群晚期糖化终产物受体( RAGE)基因-429T/C多态性对其mRNA和蛋白表达的影响。方法选取2012年8—12月糖耐量正常的汉族人群70名,首先通过DNA直接测序分析确定携带TT基因型者57名和携带TC基因型者13名。随机数字表法选取TC基因型组11名,采用匹配原则选择TT基因型组11名,提取外周血单核细胞( PBMC),分别采用实时荧光定量PCR法和Western blotting法检测RAGE mRNA和蛋白的表达水平。结果 TT基因型组RAGE mRNA表达水平高于TC基因型组〔(1.047±0.233)与(0.740±0.209),P<0.05〕。TT基因型组RAGE蛋白表达水平高于TC基因型组〔(1.121±0.252)与(0.916±0.249),P<0.05〕。结论中国汉族人群RAGE基因-429T/C多态性可能影响RAGE mRNA和蛋白的表达,-429C等位基因可能降低 RAGE基因表达水平。
目的:探討中國漢族人群晚期糖化終產物受體( RAGE)基因-429T/C多態性對其mRNA和蛋白錶達的影響。方法選取2012年8—12月糖耐量正常的漢族人群70名,首先通過DNA直接測序分析確定攜帶TT基因型者57名和攜帶TC基因型者13名。隨機數字錶法選取TC基因型組11名,採用匹配原則選擇TT基因型組11名,提取外週血單覈細胞( PBMC),分彆採用實時熒光定量PCR法和Western blotting法檢測RAGE mRNA和蛋白的錶達水平。結果 TT基因型組RAGE mRNA錶達水平高于TC基因型組〔(1.047±0.233)與(0.740±0.209),P<0.05〕。TT基因型組RAGE蛋白錶達水平高于TC基因型組〔(1.121±0.252)與(0.916±0.249),P<0.05〕。結論中國漢族人群RAGE基因-429T/C多態性可能影響RAGE mRNA和蛋白的錶達,-429C等位基因可能降低 RAGE基因錶達水平。
목적:탐토중국한족인군만기당화종산물수체( RAGE)기인-429T/C다태성대기mRNA화단백표체적영향。방법선취2012년8—12월당내량정상적한족인군70명,수선통과DNA직접측서분석학정휴대TT기인형자57명화휴대TC기인형자13명。수궤수자표법선취TC기인형조11명,채용필배원칙선택TT기인형조11명,제취외주혈단핵세포( PBMC),분별채용실시형광정량PCR법화Western blotting법검측RAGE mRNA화단백적표체수평。결과 TT기인형조RAGE mRNA표체수평고우TC기인형조〔(1.047±0.233)여(0.740±0.209),P<0.05〕。TT기인형조RAGE단백표체수평고우TC기인형조〔(1.121±0.252)여(0.916±0.249),P<0.05〕。결론중국한족인군RAGE기인-429T/C다태성가능영향RAGE mRNA화단백적표체,-429C등위기인가능강저 RAGE기인표체수평。
Objective To investigate the effects of RAGE gene -429T/C polymorphism on its mRNA and protein ex-pression in a Chinese Han population. Methods A total of 70 Han people with normal glucose tolerance( NGT)were selected as study subjects from August 2012 to December 2012,by DNA sequencing analysis,TT genotype was found in 57 subjects,and TC genotype was found in 13 subjects,11 subjects with TT genotype and 11 subjects with TC genotype were selected. The mRNA and protein expression of RAGE in PBMC were evaluated by real-time PCR and western-blot analysis,respectively. Results The expression levels of RAGE mRNA in the subjects with TT genotype were significantly higher than those in the subjects with TC genotype〔(1. 047 ± 0. 233)vs. (0. 740 ± 0. 109),P<0. 05〕. The expression levels of RAGE protein in the subjects with TT genotype were also significantly higher than those in the subjects with TC genotype〔(1. 121 ± 0. 252)vs. (0. 916 ± 0. 249),P<0. 05〕. Conclusion The RAGE gene -429T/C polymorphism may influence expression of RAGE mRNA and protein,-429C allele may reduce RAGE gene expression levels.