中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
12期
1589-1591
,共3页
冯文莉%王艳青%杨静%奚志琴%王艳%马彦%乔祖莎
馮文莉%王豔青%楊靜%奚誌琴%王豔%馬彥%喬祖莎
풍문리%왕염청%양정%해지금%왕염%마언%교조사
标本%体液/微生物学%真菌病/诊断%聚合酶链反应%早期诊断
標本%體液/微生物學%真菌病/診斷%聚閤酶鏈反應%早期診斷
표본%체액/미생물학%진균병/진단%취합매련반응%조기진단
SUPERFICIALITY & ORIGIN%Body fluids/microbiology%Mycoses/diagnosis%Polymerase chain reaction%Early diagnosis
目的 探讨采用PCR方法检测临床体液标本真菌感染的可行性.方法 对60例可疑侵袭性真菌感染者无污染体液标本进行真菌直接镜检及培养,同时应用真菌通用引物ITS4和ITS86对临床体液标本进行PCR检测,分析二者检测结果的差异.结果 临床含真菌体液标本直接进行PCR扩增的片段大小均与阳性对照DNA扩增结果一致.且PCR扩增的阳性率与真菌直接镜检、培养方法阳性率相近[38.3% (23/60) vs 33.3% (20/60),P>0.05].结论 侵袭性真菌感染采用真菌ITS通用引物进行PCR扩增,能准确、快速地检测出致病真菌,其方法对侵袭性真菌感染的早期诊断与预后具有指导意义.
目的 探討採用PCR方法檢測臨床體液標本真菌感染的可行性.方法 對60例可疑侵襲性真菌感染者無汙染體液標本進行真菌直接鏡檢及培養,同時應用真菌通用引物ITS4和ITS86對臨床體液標本進行PCR檢測,分析二者檢測結果的差異.結果 臨床含真菌體液標本直接進行PCR擴增的片段大小均與暘性對照DNA擴增結果一緻.且PCR擴增的暘性率與真菌直接鏡檢、培養方法暘性率相近[38.3% (23/60) vs 33.3% (20/60),P>0.05].結論 侵襲性真菌感染採用真菌ITS通用引物進行PCR擴增,能準確、快速地檢測齣緻病真菌,其方法對侵襲性真菌感染的早期診斷與預後具有指導意義.
목적 탐토채용PCR방법검측림상체액표본진균감염적가행성.방법 대60례가의침습성진균감염자무오염체액표본진행진균직접경검급배양,동시응용진균통용인물ITS4화ITS86대림상체액표본진행PCR검측,분석이자검측결과적차이.결과 림상함진균체액표본직접진행PCR확증적편단대소균여양성대조DNA확증결과일치.차PCR확증적양성솔여진균직접경검、배양방법양성솔상근[38.3% (23/60) vs 33.3% (20/60),P>0.05].결론 침습성진균감염채용진균ITS통용인물진행PCR확증,능준학、쾌속지검측출치병진균,기방법대침습성진균감염적조기진단여예후구유지도의의.
Objective The aim of this study was to investigate the diagnostic impact of polymerase chain reaction (PCR) assays for fungal pathogens in fluid samples,and to evaluate the feasibility of fast PCR diagnostic method for the invasive fungal infection.Methods The sterility body fluid samples from 60 cases hospitalized immunocompromised patients with clinical underlying diseases and suspect of invasive infections with fungi between January 2008 and December 2011 were processed for microscopy and cultures.Applying fungal ITS4 and ITS86 universal primers to amplify pathogenic fungi genes from the sterility body fluid with method of rapid PCR.The results were compared between the standard and PCR methods.Results Humoral direct clinical specimens by PCR amplification of DNA fragments with the scan results were similar.Positive rate of PCR test with clinical body fluid samples and the traditional fungal cultivation was similar.There was no significant statistical difference [38.3% (23/60) vs 33.3% (20/60),P > 0.05].Conclusions PCR test is feasibility with clinical fungal diagnosis from directly humoral specimens.To amplify the clinical sterility body fluid samples with ITS fungal universal primers and PCR method might provide an accurate and rapid approach to detect the pathogenic fungi.Its methods on early diagnosis and prognosis of invasive fungal infections are of guiding significance.