CAJ | 학술논문

目的探讨解整合素金属蛋白酶33(ADAM33)基因V4、T2位点多态性与儿童哮喘易感性及严重程度的相关性.方法采用以医院为基础的病例对照研究(110例哮喘患儿和144例对照)方法,四位点用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)技术进行基因分型,应用MDR软件分析基因各位点之间交互作用.结果哮喘组V4位点C、T2位点A等位基因频率均高于对照组(P ＜0.01,OR =2.36 ～2.96,95％ CI:1.56-1.78 ～3.56-4.94).轻中度和重度哮喘组中V4位点GC基因型频率均高于对照组(P＜0.05),重度哮喘组CC基因型频率高于对照组(P＜0.05)；T2位点GA基因型在重度哮喘组中分布高于对照组(P＜0.01),轻中度哮喘组AA基因型频率高于对照组(P＜0.05)；MDR交互作用分析显示,ADAM33基因V4、T2位点构成最佳模型.结论 ADAM33基因V4、T2位点与儿童哮喘发生及其严重程度相关,同时ADAM33基因V4、T2位点均具有明显交互作用.
목적 탐토해정합소금속단백매33(ADAM33)기인V4、T2위점다태성여인동효천역감성급엄중정도적상관성.방법 채용이의원위기출적병례대조연구(110례효천환인화144례대조)방법,사위점용취합매련반응-한제성편단장도다태성분석(PCR-RFLP)기술진행기인분형,응용MDR연건분석기인각위점지간교호작용.결과 효천조V4위점C、T2위점A등위기인빈솔균고우대조조(P ＜0.01,OR =2.36 ～2.96,95％ CI:1.56-1.78 ～3.56-4.94).경중도화중도효천조중V4위점GC기인형빈솔균고우대조조(P＜0.05),중도효천조CC기인형빈솔고우대조조(P＜0.05)；T2위점GA기인형재중도효천조중분포고우대조조(P＜0.01),경중도효천조AA기인형빈솔고우대조조(P＜0.05)；MDR교호작용분석현시,ADAM33기인V4、T2위점구성최가모형.결론 ADAM33기인V4、T2위점여인동효천발생급기엄중정도상관,동시ADAM33기인V4、T2위점균구유명현교호작용.
Objective To verify the association of a disintegrin and metalloproteinase domain 33 (ADAM33) polymorphisms in childhood asthma susceptibility and severity in patients with moderate and severe asthma.Methods A total of 144 controls and 110 asthmatic patients were recruited for this hospitalbased case-control study.Two polymorphic sites (V4,T2) were genotyped using the polymerase chain reaction-restriction fragment length polymorphism method.The gene-gene interactions were analyzed with the multifactor dimensionality reduction (MDR) software.Results The allele frequencies of three SNPs (V4,T2) of ADAM33 in childhood asthma group were significantly higher than control group (P ＜ 0.01,OR =2.36 ～ 2.96,95％ CI:1.56-1.78 ～ 3.56-4.94).For the SNPs V4,GC genotype frequencies of both moderate and severe groups were significantly higher from the control group (P ＜ 0.05) ; the CC genotype frequencies in severe group were significantly higher than control group (P ＜ 0.05) ; the genotype GA of T2 locus in severe group and AA genotype frequencies in moderate group were significantly higher than control group (P ＜0.01 ～0.05) ; there were no significant differences for both allele and genotype frequencies of S2 locus between the childhood asthma and control group (P ＞ 0.05).By MDR analysis,the best interaction model was the four-factor model that the V4,T2 genotypes were the subgroup to predict asthma risk.Conclusions Our results highlight the role of ADAM33 as a susceptibility gene for childhood asthma,and the interactions among ADAM33 V4 and T2 are also associated with childhood asthma.