中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
6期
721-724
,共4页
江志超%唐发清%田道法%李梅芳
江誌超%唐髮清%田道法%李梅芳
강지초%당발청%전도법%리매방
鼻咽肿瘤/免疫学%鼻咽肿瘤/病理学%T淋巴细胞,调节性%翼螺旋转录因子类%转化生长因子β1%白细胞介素10
鼻嚥腫瘤/免疫學%鼻嚥腫瘤/病理學%T淋巴細胞,調節性%翼螺鏇轉錄因子類%轉化生長因子β1%白細胞介素10
비인종류/면역학%비인종류/병이학%T림파세포,조절성%익라선전록인자류%전화생장인자β1%백세포개소10
Nasopharyngeal neoplasms/immunology%Nasopharyngeal neoplasms/pathology%Tlymphocytes,regulatory%Winged-helix transcription factors%Transforming growth factor betal%Interleukin-10
目的 探讨中晚期鼻咽癌患者外周血CD4+CD25+T调节细胞比例及转录因子叉头状螺旋转录因子(forkhead transcription factor 3,Foxp3)和血清细胞因子转化生长因子β1(TGF-β1)和IL-10水平的免疫病理意义.方法 分别应用流式细胞术(FCM)、逆转录-聚合酶链反应(RT-PCR)、ELISA方法检测中晚期鼻咽癌患者外周血单个核细胞中CD4+CD25+T调节细胞比例、Foxp3转录活性和血清TGF-β1、IL-10表达水平,并与健康人相应指标进行比较,分析其免疫病理意义.结果 中晚期鼻咽癌患者外周血CD4+ CD25+T调节细胞比例[(4.23±0.53)％vs(2.65±0.31)％,t=8.60,P＜0.01]、Foxp3转录活性(3.699±0.309 vs 1.109±0.146,t’=31.08,P＜0.05)、TGF-β1[(645.56±39.61) pg/ml vs(488.82±36.91)pg/ml,t =10.27,P＜0.01]及IL-10[(1.27±0.21)pg/ml vs (0.68±0.08) pg/ml,t’=10.61,P＜0.05]表达水平均明显高于健康人.结论 CD4+CD25+T调节细胞、转录因子Foxp3和细胞因子TGF-β1、IL-10共同构成一个正反馈体系,促进了中晚期鼻咽癌患者肿瘤微环境中免疫耐受现象的发展及免疫逃逸的发生过程.
目的 探討中晚期鼻嚥癌患者外週血CD4+CD25+T調節細胞比例及轉錄因子扠頭狀螺鏇轉錄因子(forkhead transcription factor 3,Foxp3)和血清細胞因子轉化生長因子β1(TGF-β1)和IL-10水平的免疫病理意義.方法 分彆應用流式細胞術(FCM)、逆轉錄-聚閤酶鏈反應(RT-PCR)、ELISA方法檢測中晚期鼻嚥癌患者外週血單箇覈細胞中CD4+CD25+T調節細胞比例、Foxp3轉錄活性和血清TGF-β1、IL-10錶達水平,併與健康人相應指標進行比較,分析其免疫病理意義.結果 中晚期鼻嚥癌患者外週血CD4+ CD25+T調節細胞比例[(4.23±0.53)％vs(2.65±0.31)％,t=8.60,P＜0.01]、Foxp3轉錄活性(3.699±0.309 vs 1.109±0.146,t’=31.08,P＜0.05)、TGF-β1[(645.56±39.61) pg/ml vs(488.82±36.91)pg/ml,t =10.27,P＜0.01]及IL-10[(1.27±0.21)pg/ml vs (0.68±0.08) pg/ml,t’=10.61,P＜0.05]錶達水平均明顯高于健康人.結論 CD4+CD25+T調節細胞、轉錄因子Foxp3和細胞因子TGF-β1、IL-10共同構成一箇正反饋體繫,促進瞭中晚期鼻嚥癌患者腫瘤微環境中免疫耐受現象的髮展及免疫逃逸的髮生過程.
목적 탐토중만기비인암환자외주혈CD4+CD25+T조절세포비례급전록인자차두상라선전록인자(forkhead transcription factor 3,Foxp3)화혈청세포인자전화생장인자β1(TGF-β1)화IL-10수평적면역병리의의.방법 분별응용류식세포술(FCM)、역전록-취합매련반응(RT-PCR)、ELISA방법검측중만기비인암환자외주혈단개핵세포중CD4+CD25+T조절세포비례、Foxp3전록활성화혈청TGF-β1、IL-10표체수평,병여건강인상응지표진행비교,분석기면역병리의의.결과 중만기비인암환자외주혈CD4+ CD25+T조절세포비례[(4.23±0.53)％vs(2.65±0.31)％,t=8.60,P＜0.01]、Foxp3전록활성(3.699±0.309 vs 1.109±0.146,t’=31.08,P＜0.05)、TGF-β1[(645.56±39.61) pg/ml vs(488.82±36.91)pg/ml,t =10.27,P＜0.01]급IL-10[(1.27±0.21)pg/ml vs (0.68±0.08) pg/ml,t’=10.61,P＜0.05]표체수평균명현고우건강인.결론 CD4+CD25+T조절세포、전록인자Foxp3화세포인자TGF-β1、IL-10공동구성일개정반궤체계,촉진료중만기비인암환자종류미배경중면역내수현상적발전급면역도일적발생과정.
Objective To investigate the implications of ratio of the CD4+ and CD25+ positive regulatory T cells (CD4+CD25+Tregs) in peripheral blood mononuclear cells (PBMC) and its associated regulatory factors such as forkhead transcription factor 3 (Foxp3) mRNA transcriptional activity in PBMC,serum levels of transforming growth factor beta-1 (TGF-β1),and interleukin 10 (IL-10) in the immunopathology of patients with middle to late staged nasopharyngeal carcinoma (NPC) based on a clinical trial.Methods In this study,18 NPC cases at middle to late stage as observing group and 10 healthy persons as control group were included to detect their ratio of the CD4+CD25+Tregs in the PBMC with flow cytometry (FCM) technique,transcriptional activity of Foxp3 with RT-PCR procedure,and serum levels of TGF-β1 and IL-10 with enzyme-linked immunosorbent assay (ELISA) method.A comparative analysis was used to explore their implications in the immunopathological correlation of NPC cases with their lesion.Results The ratio of the CD4+CD25+Tregs to total CD4+T cells in PBMC was significantly increased [(4.23 ±0.53)％ vs (2.65 ±0.31)％,t =8.60,P ＜0.01],accompanied with significantly elevated levels of Foxp3 transcription in PBMC (3.699 ± 0.309 vs 1.109 ± 0.146,t' =31.08,P ＜ 0.05],and serum contents of TGF-β1 [(645.56 ± 39.61) pg/ml vs (488.82 ± 36.91) pg/ml,t =10.27,P ＜ 0.01] and IL-10 [(1.27 ± 0.21) pg/ml vs (0.68 ± 0.08) pg/ml,t' =10.61,P ＜ 0.05] in these patients,when compared with that of healthy controls.Conclusions It may be true that CD4 + CD25 + Tregs,transcriptional regulatory factor Foxp3,and cytokines TGF-β1 as well as IL-10 altogether were composed of a regulating system in a positive feedback way to promote the developing process of immunotolerance phenomena in the tumor microenvironment and the initiation of immunoescape among patients with middle to late staged nasopharyngeal carcinoma.
