中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
6期
740-743
,共4页
丁酸类/治疗应用%脑损伤/药物疗法%脑损伤/化学诱导%链球菌溶血素类/副作用%高迁移率族蛋白质类%NF-κB
丁痠類/治療應用%腦損傷/藥物療法%腦損傷/化學誘導%鏈毬菌溶血素類/副作用%高遷移率族蛋白質類%NF-κB
정산류/치료응용%뇌손상/약물요법%뇌손상/화학유도%련구균용혈소류/부작용%고천이솔족단백질류%NF-κB
Butyric acids/therapeutic use%Brain injuries/drug therapy%Brain injuries/chemically induced%Streptolysins/adverse effects%High mobility group proteins%NF-kappa B
目的 探讨正丁酸钠(SB)对肺炎链球菌溶血素(PLY)致幼年大鼠脑损伤的保护作用.方法 96只1月龄SD大鼠按随机数字表法分为对照组(NS组,n=32),颈外动脉注射生理盐水；PLY脑损伤组(PLY组,n=32),颈外动脉注射PLY;SB治疗组(SB组,n=32),颈外动脉注射PLY后即刻静脉注射SB.于注射药物后24、48 h取上腔静脉血留取备用,并处死动物,制备脑组织标本,干湿质量法测定其脑组织含水量(BWC),甲酰胺法测定伊文思兰(EB)水平,采用酶联免疫吸附试验分析高迁移率族蛋白B1(HMGB1)、核转录因子(NF-κB)水平.结果 PLY组、SB组各时间点脑组织BWC、EB水平及血HMGB1、NF-κB水平均明显高于NS组(P＜0.05),且SB组各时间点上述指标均低于PLY组(P＜0.05).PLY组及SB组中HMGB1水平与NF-κB、BWC、EB水平呈正相关(r =0.817～0.917,P＜0.05).结论 正丁酸钠可能通过抑制NF-κB活化减轻HMGB1的表达而对肺炎链球菌溶血素致脑损伤具有保护作用.
目的 探討正丁痠鈉(SB)對肺炎鏈毬菌溶血素(PLY)緻幼年大鼠腦損傷的保護作用.方法 96隻1月齡SD大鼠按隨機數字錶法分為對照組(NS組,n=32),頸外動脈註射生理鹽水；PLY腦損傷組(PLY組,n=32),頸外動脈註射PLY;SB治療組(SB組,n=32),頸外動脈註射PLY後即刻靜脈註射SB.于註射藥物後24、48 h取上腔靜脈血留取備用,併處死動物,製備腦組織標本,榦濕質量法測定其腦組織含水量(BWC),甲酰胺法測定伊文思蘭(EB)水平,採用酶聯免疫吸附試驗分析高遷移率族蛋白B1(HMGB1)、覈轉錄因子(NF-κB)水平.結果 PLY組、SB組各時間點腦組織BWC、EB水平及血HMGB1、NF-κB水平均明顯高于NS組(P＜0.05),且SB組各時間點上述指標均低于PLY組(P＜0.05).PLY組及SB組中HMGB1水平與NF-κB、BWC、EB水平呈正相關(r =0.817～0.917,P＜0.05).結論 正丁痠鈉可能通過抑製NF-κB活化減輕HMGB1的錶達而對肺炎鏈毬菌溶血素緻腦損傷具有保護作用.
목적 탐토정정산납(SB)대폐염련구균용혈소(PLY)치유년대서뇌손상적보호작용.방법 96지1월령SD대서안수궤수자표법분위대조조(NS조,n=32),경외동맥주사생리염수；PLY뇌손상조(PLY조,n=32),경외동맥주사PLY;SB치료조(SB조,n=32),경외동맥주사PLY후즉각정맥주사SB.우주사약물후24、48 h취상강정맥혈류취비용,병처사동물,제비뇌조직표본,간습질량법측정기뇌조직함수량(BWC),갑선알법측정이문사란(EB)수평,채용매련면역흡부시험분석고천이솔족단백B1(HMGB1)、핵전록인자(NF-κB)수평.결과 PLY조、SB조각시간점뇌조직BWC、EB수평급혈HMGB1、NF-κB수평균명현고우NS조(P＜0.05),차SB조각시간점상술지표균저우PLY조(P＜0.05).PLY조급SB조중HMGB1수평여NF-κB、BWC、EB수평정정상관(r =0.817～0.917,P＜0.05).결론 정정산납가능통과억제NF-κB활화감경HMGB1적표체이대폐염련구균용혈소치뇌손상구유보호작용.
Objective To investigate the protective effect of sodium butyrate on brain injury induced by pneumolysin of infantile rats.Methods Ninety-six normal healthy 1-month-old Spragne-Dawley (SD) rats were randomly divided into three groups,including pneumolysin (PLY) group (n =32),in which rat was injected PLY via external carotid; Normal saline (NS) group (n =32),injected NS via external carotid; sodium butyrate (SB) group (n =32),after injecting PLY,immediately administrated SB via venous.In the injection the 24th h and 48th h,superior vena cava blood was taken,and the animals were sacrificed,and brain tissue samples were prepared.The brain water content (BWC) was recorded by measuring both wet and dry weight,the Evans blue (EB) level was measured by the formamide method.The serum levels of high mobility group protein B1 (HMGB1) and nuclear factor kappa B (NF-κB) were measured by enzyme-linked immunosorbent assay (ELISA).Results In PLY group,brain tissue BWC,EB level,and the blood level of HMGB1 and NF-κB were increased significantly compared with the NS group at each time point,the difference was statistically significant (P ＜ 0.05).These indices were lower in the SB group compared with PLY group,the difference was statistically significant (P ＜ 0.05).The positive correlation was gotten between HMGB1 and NF-κB,BWC,EB levels in the PLY group and SB group (r =0.817 ～0.917,P ＜ 0.05).Conclusions SB has neuroprotective effect in brain injury induced by PLY,which maybe relevant to inhibition of NF-κB activation and suppression of HMGB1 expression.
