CAJ | 학술논문

目的研究甘露醇甘油注射液(含15％甘露醇和5％甘油)对体外兔红细胞和大鼠体内红细胞溶血的影响.方法采用体外试管法观察甘露醇甘油注射液对正常实验兔红细胞的溶血作用；应用邻-甲联苯胺氧化显色法测定血浆游离血红蛋白含量,体内试验测定SD大鼠红细胞溶血.取血浆测定游离血红蛋白和间接胆红素含量.结果与生理盐水组比较,RBC+ 5％甘油组分光光度法OD值、邻联甲苯胺法测定血红蛋白OD值1.6ml、2.0ml管差异有统计学意义(P＜0.01),5％甘油+ RBC组各管差异均有统计学意义(P＜0.01),而RBC+甘露醇甘油组、甘露醇甘油+RBC组与生理盐水组比较差异均无统计学意义(P＞0.05).按6、10 ml/min给药速度,30 ml/kg给药体积静脉给予大鼠相应受试品2、24h后,甘露醇甘油注射液组血浆游离血红蛋白、间接胆红素与生理盐水组比较差异无统计学意义(P＞0.05)；5％甘油组24h间接胆红素与生理盐水组比较差异有统计学意义(P＜0.05).结论甘露醇甘油注射液在体外、体内对红细胞均无溶血作用.
목적 연구감로순감유주사액(함15％감로순화5％감유)대체외토홍세포화대서체내홍세포용혈적영향.방법 채용체외시관법관찰감로순감유주사액대정상실험토홍세포적용혈작용；응용린-갑련분알양화현색법측정혈장유리혈홍단백함량,체내시험측정SD대서홍세포용혈.취혈장측정유리혈홍단백화간접담홍소함량.결과 여생리염수조비교,RBC+ 5％감유조분광광도법OD치、린련갑분알법측정혈홍단백OD치1.6ml、2.0ml관차이유통계학의의(P＜0.01),5％감유+ RBC조각관차이균유통계학의의(P＜0.01),이RBC+감로순감유조、감로순감유+RBC조여생리염수조비교차이균무통계학의의(P＞0.05).안6、10 ml/min급약속도,30 ml/kg급약체적정맥급여대서상응수시품2、24h후,감로순감유주사액조혈장유리혈홍단백、간접담홍소여생리염수조비교차이무통계학의의(P＞0.05)；5％감유조24h간접담홍소여생리염수조비교차이유통계학의의(P＜0.05).결론 감로순감유주사액재체외、체내대홍세포균무용혈작용.
Objective To investigate the effect of the mannitol-glycerol injection (containing 15％ mannitol and 15％ glycerol) on hemolysis of red cells in rabbits and rats.Methods By the test tube method in vitro to observe hemolytic effect of mannitol-glycerol injection in the normal rabbit red blood ceils.The neighborhood-a oxidation benzidine chromogenic method was used to determine the levels of plasma free hemoglobin content.The SD rats'red blood cell hemolysis in vivo was determined.The plasma free hemoglobin and hemobilirubin was determined.Results Compared with the sodium chloride group,the 1.6 ml,2.0 ml tubes'OD values of RBC + 5 ％ glycerol group by testing in spectrophotometric method,and the 1.6 ml,2.0 ml tubes'OD values of 5％ glycerol group by testing in ortho-tolidine method had statistically significant differences (P ＜0.01).There were significant differences of each tube in 5％ glycerol + RBC group (P＜0.01).While RBC + mannitol glycerol group and mannitol,glycerol + RBC group compared with the Sodium Chloride group showed no significant difference (P ＞0.05).By 6,10 ml/min dose rate,30 ml/kg volume intravenously administered to rats corresponding substances,there was no statistical significance between mannitol and glycerin injection group's plasma free hemoglobin and hemobilirubin with sodium chloride group after 2,24 hours (P ＞0.05); 5％ glycerol group's 24h hemobilirubin compared with the sodium chloride group has statistically significant differences (P ＜ 0.05).Conclusions The mannitol-glycerol injection had no hemolytic effect on erythrocytes in vivo and in vitro.