CAJ | 학술논문

目的观察芍药苷对高脂饲养大鼠胰岛素敏感性和血脂谱的影响,并研究其作用机制.方法将雄性SD大鼠按随机数字表法分为正常组、高脂饲养组、芍药苷低剂量组和芍药苷高剂量组,除正常组喂食普通饲料外,其余各组喂食高脂饲料,芍药苷干预组另分别给予低、高剂量芍药苷腹腔注射,第6周末各组大鼠禁食12 h后处死,采血测定血清甘油三酯(TG)、总胆固醇(TC)、游离脂肪酸(FFA)、空腹血糖(FBG)及胰岛素(Ins)并计算胰岛素敏感指数(ISI),剪取附睾脂肪,定量PCR方法检测附睾脂肪组织肿瘤坏死因子α(TNFα)、葡萄糖转运子4(Glut4)表达.结果芍药苷高剂量组大鼠与高脂组相比,附睾脂肪重量减少,FBG、Ins、FFA水平降低(P＜0.05),ISI明显改善(-5.84 ±0.24 vs-6.44 ±0.25,P＜0.05),芍药苷低剂量组亦有类似趋势.高脂组大鼠脂肪组织内TNFα mRNA表达上调,Glut4 mRNA表达下调,两用药干预组TNFα mRNA水平较高脂组有明显降低(P＜0.05),芍药苷高剂量组Glug mRNA表达显著高于高脂组(P＜0.05).结论芍药苷能够减少高脂饲养大鼠内脏脂肪含量,抑制脂肪组织TNFα mRNA的表达、上调Glut4 mRNA水平,从而降低血糖,改善胰岛素抵抗.
목적 관찰작약감대고지사양대서이도소민감성화혈지보적영향,병연구기작용궤제.방법 장웅성SD대서안수궤수자표법분위정상조、고지사양조、작약감저제량조화작약감고제량조,제정상조위식보통사료외,기여각조위식고지사료,작약감간예조령분별급여저、고제량작약감복강주사,제6주말각조대서금식12 h후처사,채혈측정혈청감유삼지(TG)、총담고순(TC)、유리지방산(FFA)、공복혈당(FBG)급이도소(Ins)병계산이도소민감지수(ISI),전취부고지방,정량PCR방법검측부고지방조직종류배사인자α(TNFα)、포도당전운자4(Glut4)표체.결과 작약감고제량조대서여고지조상비,부고지방중량감소,FBG、Ins、FFA수평강저(P＜0.05),ISI명현개선(-5.84 ±0.24 vs-6.44 ±0.25,P＜0.05),작약감저제량조역유유사추세.고지조대서지방조직내TNFα mRNA표체상조,Glut4 mRNA표체하조,량용약간예조TNFα mRNA수평교고지조유명현강저(P＜0.05),작약감고제량조Glug mRNA표체현저고우고지조(P＜0.05).결론 작약감능구감소고지사양대서내장지방함량,억제지방조직TNFα mRNA적표체、상조Glut4 mRNA수평,종이강저혈당,개선이도소저항.
Objective To observe the effect of paeoniflorin on insulin resistance in rats fed with high-fat diet and to investigate the possible mechanisms.Methods Male Sprague Dawley (SD) rats were randomized into 4 groups:normal control,high-fat diet,high-dosage paeoniflorin (HDP group),and lowdosage paeoniflorin (LDP group).The control group was fed with ordinary diet,while the others with highfat diet,paeoniflorin intervention groups were given low-or high-dosage paeoniflorin by intraperitoneal injection.After 6 weeks,fasting serum triacylglycerol (TG),total cholesterol (TC),free fatty acids (FFA),fasting blood glucose (FBG),and insulin were determined.Insulin sensitivity index (ISI) were calculated and then the animals were sacrificed to acquire epididymal fat mass.The tumor necrosis factor alpha (TNFα) and glucose transporter 4 (Glut4) expressions in adipose tissue were detected by quantitative realtime polymerase chain reaction(PCR).Results Compared with high-fat fed group,HDP group had lower epididymal fat pad weight,reduced level of FBG,insulin and FFA (P ＜0.05) and improved ISI(-5.84 ± 0.24 vs-6.44 ± 0.25,P ＜ 0.05).LDP group had similar trends.In adipose tissue,the TNFα expression in LDP and HDP group was lower,Glut4 expression in HDP group was higher than that of high-fat fed group (P ＜ 0.05).Conclusion Paeoniflorin can reduce visceral adipose content,inhibit TNFα expression and increase Glut4 expression in adipose tissue,eventually lower glucose,and improve insulin resistance caused by high-fat diet.