CAJ | 학술논문

目的探讨Wnt1蛋白和β-catenin蛋白在乳腺浸润性导管癌发生发展中的作用及其与临床病理特征的关系.方法应用免疫组化SP法检测Wnt1和β-catenin蛋白在15例乳腺癌癌旁组织、25例乳腺增生组织和80例乳腺浸润性导管癌组织中的表达；采用SPSS 17.0统计软件包进行分析,计数资料用x2检验,相关性分析用Spearman检验,以α=0.05为检验水准.结果乳腺癌组Wnt1的阳性表达率和β-catenin的异常表达率分别为62.5％ (50/80)、68.8％ (55/80),显著高于增生组[28％ (7/25)、20％ (5/25)]和癌旁组[13.33％ (2/15)、13.33％ (2/15)],差异均具有统计学意义(P＜0.01).乳腺浸润性导管癌中Wnt1的阳性表达和β-catenin的异常表达均与淋巴结转移有关(x2 =5.10,4.87,P＜0.05),β-catenin的异常表达还与乳腺浸润性导管癌的组织学分级有关(x2=5.61,P ＜0.05).乳腺浸润性导管癌中Wnt1和β-catenin蛋白表达呈正相关(r=0.313,P ＜0.05).结论 Wnt1蛋白高表达与β-catenin蛋白异常表达在乳腺癌变过程中起着重要作用,并与淋巴结转移有密切关系.
목적 탐토Wnt1단백화β-catenin단백재유선침윤성도관암발생발전중적작용급기여림상병리특정적관계.방법 응용면역조화SP법검측Wnt1화β-catenin단백재15례유선암암방조직、25례유선증생조직화80례유선침윤성도관암조직중적표체；채용SPSS 17.0통계연건포진행분석,계수자료용x2검험,상관성분석용Spearman검험,이α=0.05위검험수준.결과 유선암조Wnt1적양성표체솔화β-catenin적이상표체솔분별위62.5％ (50/80)、68.8％ (55/80),현저고우증생조[28％ (7/25)、20％ (5/25)]화암방조[13.33％ (2/15)、13.33％ (2/15)],차이균구유통계학의의(P＜0.01).유선침윤성도관암중Wnt1적양성표체화β-catenin적이상표체균여림파결전이유관(x2 =5.10,4.87,P＜0.05),β-catenin적이상표체환여유선침윤성도관암적조직학분급유관(x2=5.61,P ＜0.05).유선침윤성도관암중Wnt1화β-catenin단백표체정정상관(r=0.313,P ＜0.05).결론 Wnt1단백고표체여β-catenin단백이상표체재유선암변과정중기착중요작용,병여림파결전이유밀절관계.
Objective To investigate the expressions of Wnt1 and β-catenin proteins in mammary cancer and its relationship with clinical pathological characteristics,and explore its role in the pathogenesis of breast cancer.Methods Immunoshistochemisty (SP method) was used to detect the expressions of Wnt1 and β-catenin proteins in adjacent tissue of breast cancer (n =15),breast hyperplasia tissues (n =25),and breast in vasive ductal carcinoma (n =80).SPSS 17.0 statistical software was used for analysis,such as count data with x2 test and correlation analysis with Spearman test,with significance level of α =0.05.Results The positive rate of Wnt1 and β-catenin in breast cancer group [62.5％ (50/80) and 68.8 (55/80)] was significantly higher than that in hyperplasia group [28％ (7/25) and 20％ (5/25)]and in borderline group [13.33 (2/15) and 13.33 (2/15)] with a significant difference (P ＜ 0.01).Positive expression of Wnt1 and abnormal expression of β-catenin in breast invasive ductal carcinoma were related to lymphatic metastasis (x2 =5.10,4.87,P ＜ 0.05).Abnormal expression of β-catenin was also related to the histological grading of breast invasive ductal carcinoma (x2 =5.61,P ＜ 0.05).The expressions of Wnt1 and β-catenin proteins in invasive ductal carcinoma showed a positive correlation (r =0.313,P ＜ 0.01).Conclusions The high level expression of Wnt1 protein and the abnormal expression of β-catenin protein might play an important role in breast carcinogenesis,and was related to lymph node metastasis.