中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
10期
1334-1337
,共4页
李玮浩%赵松%崔广晖%郭海周
李瑋浩%趙鬆%崔廣暉%郭海週
리위호%조송%최엄휘%곽해주
角蛋白质类/代谢%食管肿瘤/病理学%肿瘤,鳞状细胞/病理学%免疫组织化学
角蛋白質類/代謝%食管腫瘤/病理學%腫瘤,鱗狀細胞/病理學%免疫組織化學
각단백질류/대사%식관종류/병이학%종류,린상세포/병이학%면역조직화학
Keratins/metabolism%Esophageal neoplasms/pathology%Neoplasms,squamous cell/pathology%Immunohistochemistry
目的 研究角蛋白34βE12在不同食管组织中的表达及其与临床生物学行为的关系,进而探讨其在食管癌发生、发展中的作用机制.方法 采用免疫组织化学方法分析252例食管鳞状细胞癌、66例食管原位癌及106例癌旁正常食管黏膜组织中角蛋白34βE12的表达情况,并根据临床资料评价其表达与生物学行为的关系;另收集其中60例新鲜食管癌组织及其癌旁正常食管组织,采用Western印迹法测定组织中角蛋白34βE12的表达情况.结果 (1)角蛋白34βE12在食管鳞癌、原位癌及癌旁正常食管组织中的阳性表达率分别为85.7%、54.5%和25.7%,差异均具有统计学意义(P<0.01).(2)角蛋白34βE12在食管鳞癌临床Ⅰ期、Ⅱ期及Ⅲ期的表达率分别为76.5%,84.7%和96.3%,癌组织的表达强度随着肿瘤临床分期的升高而逐渐增强,差异具有统计学意义(P=0.038);淋巴结转移组角蛋白34βE12的表达明显高于无淋巴结转移组(P<0.01).(3)Western印迹法结果进一步证实食管鳞癌组织的角蛋白34βE12的表达明显高于癌旁正常食管组织(P<0.01).结论 角蛋白34βE12的高表达可能参与了食管癌的发生、发展过程;角蛋白34βE12的表达与食管癌临床分期有一定的关系,其很可能成为潜在的治疗靶点及判断食管癌预后的有价值的肿瘤标记物.
目的 研究角蛋白34βE12在不同食管組織中的錶達及其與臨床生物學行為的關繫,進而探討其在食管癌髮生、髮展中的作用機製.方法 採用免疫組織化學方法分析252例食管鱗狀細胞癌、66例食管原位癌及106例癌徬正常食管黏膜組織中角蛋白34βE12的錶達情況,併根據臨床資料評價其錶達與生物學行為的關繫;另收集其中60例新鮮食管癌組織及其癌徬正常食管組織,採用Western印跡法測定組織中角蛋白34βE12的錶達情況.結果 (1)角蛋白34βE12在食管鱗癌、原位癌及癌徬正常食管組織中的暘性錶達率分彆為85.7%、54.5%和25.7%,差異均具有統計學意義(P<0.01).(2)角蛋白34βE12在食管鱗癌臨床Ⅰ期、Ⅱ期及Ⅲ期的錶達率分彆為76.5%,84.7%和96.3%,癌組織的錶達彊度隨著腫瘤臨床分期的升高而逐漸增彊,差異具有統計學意義(P=0.038);淋巴結轉移組角蛋白34βE12的錶達明顯高于無淋巴結轉移組(P<0.01).(3)Western印跡法結果進一步證實食管鱗癌組織的角蛋白34βE12的錶達明顯高于癌徬正常食管組織(P<0.01).結論 角蛋白34βE12的高錶達可能參與瞭食管癌的髮生、髮展過程;角蛋白34βE12的錶達與食管癌臨床分期有一定的關繫,其很可能成為潛在的治療靶點及判斷食管癌預後的有價值的腫瘤標記物.
목적 연구각단백34βE12재불동식관조직중적표체급기여림상생물학행위적관계,진이탐토기재식관암발생、발전중적작용궤제.방법 채용면역조직화학방법분석252례식관린상세포암、66례식관원위암급106례암방정상식관점막조직중각단백34βE12적표체정황,병근거림상자료평개기표체여생물학행위적관계;령수집기중60례신선식관암조직급기암방정상식관조직,채용Western인적법측정조직중각단백34βE12적표체정황.결과 (1)각단백34βE12재식관린암、원위암급암방정상식관조직중적양성표체솔분별위85.7%、54.5%화25.7%,차이균구유통계학의의(P<0.01).(2)각단백34βE12재식관린암림상Ⅰ기、Ⅱ기급Ⅲ기적표체솔분별위76.5%,84.7%화96.3%,암조직적표체강도수착종류림상분기적승고이축점증강,차이구유통계학의의(P=0.038);림파결전이조각단백34βE12적표체명현고우무림파결전이조(P<0.01).(3)Western인적법결과진일보증실식관린암조직적각단백34βE12적표체명현고우암방정상식관조직(P<0.01).결론 각단백34βE12적고표체가능삼여료식관암적발생、발전과정;각단백34βE12적표체여식관암림상분기유일정적관계,기흔가능성위잠재적치료파점급판단식관암예후적유개치적종류표기물.
Objective To investigate the expression of cytokeratin 34βE12 in esophageal squamous cell carcinoma (ESCC),and its mechanism of action in the process of occurrence and development of an ESCC.Methods Immunohistochemistry was used to analyze the expression of cytokeratin 34βE12 in 252 ESCC patients,66 patients with esophageal carcinoma in situ,and 106 patients with adjacent normal esophageal mucosa before the relationship between its expression and biological behavior was evaluated on the basis of complete clinical information.In addition,Western blotting was used to determine the expression of cytokeratin 34βE12 in 60 patients with esophageal cancer and adjacent normal esophageal tissues.Results (1)The positive rate of caveolin-1 in ESCC,carcinoma in situ,and adjacent normal tissues was 85.7%,54.5%,and 25.7%,respectively.The difference between them was statistically significant (P <0.01).(2)The positive rate of cytokeratin 34βE12 in stages Ⅰ,Ⅱ,Ⅲ of ESCC was 76.5%,84.7%,and 96.3%,respectively.The expression intensity of cytokeratin 34βE12 in carcinoma tissue was gradually increased with the advance of clinical stages with a statistically significant difference (P =0.038).The positive rate of cytokeratin 34βE12 with group of lymph node metastasis was significantly higher than those without lymph node metastasis (P < 0.01).(3)Western blotting results further confirmed that the expression of cytokeratin 34βE12 in ESCC was significantly higher than that in adjacent normal esophageal tissue (P <0.01).Conclusions The high expression of caveolin-1 might be involved in the occurrence and development of esophageal cancer.The expression of cytokeratin 34βE12 was correlated with the clinical stage of esophageal cancer.cytokeratin 34βE12 was a potential therapeutic target and a valuable prognostic indicator of esophageal cancer progression.