中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
10期
1349-1352
,共4页
高迁移率族蛋白质类%子宫内膜异位症/诊断%子宫内膜异位症/治疗%卵巢
高遷移率族蛋白質類%子宮內膜異位癥/診斷%子宮內膜異位癥/治療%卵巢
고천이솔족단백질류%자궁내막이위증/진단%자궁내막이위증/치료%란소
High mobility group proteins%Endometriosis/diagnosis%Endometriosis/therapy%Ovary
目的 研究高迁移率族蛋白1(HMGB1)在卵巢子宫内膜异位症中的表达,为子宫内膜异位症的早期诊断、治疗及预后提供新的思路.方法 采用RT-PCR和western-blotting检测HMGB1 mRNA和HMGB1蛋白在69例卵巢子宫内膜异位症患者异位子宫内膜和在位内膜及69例非子宫内膜异位症患者的在位内膜中(对照组)的表达情况.结果 HMGB1 mRNA在卵巢异位子宫内膜、在位内膜和非异位症的在位内膜均有表达,其表达灰度值分别为:0.5438±0.0565、0.3016±0.0614、0.1536±0.0444；HMGB1蛋白在三组内膜中表达的灰度值分别为:0.8399±0.0287、0.4010±0.0643、0.1999±0.0716.三组HMGB1 mRNA、HMGB1蛋白的表达水平比较差异均有统计学意义(F=8.756,P＜0.05).HMGB1在各组不同月经时期表达水平比较差异均无统计学意义(P＞0.05).卵巢子宫内膜异位症患者临床分期高级别异位内膜中HMGB1表达水平高于低级别者(t=10.28,P＜0.01),与肿瘤大小、患者年龄无明显相关(P＞0.05).结论 HMGB1在卵巢子宫内膜异位、在位内膜中呈高表达,且临床分期级别越高其表达增强,其可能在卵巢子宫内膜异位症的发生、发展中起着重要的作用,可作为子宫内膜异位症的生物标记物.
目的 研究高遷移率族蛋白1(HMGB1)在卵巢子宮內膜異位癥中的錶達,為子宮內膜異位癥的早期診斷、治療及預後提供新的思路.方法 採用RT-PCR和western-blotting檢測HMGB1 mRNA和HMGB1蛋白在69例卵巢子宮內膜異位癥患者異位子宮內膜和在位內膜及69例非子宮內膜異位癥患者的在位內膜中(對照組)的錶達情況.結果 HMGB1 mRNA在卵巢異位子宮內膜、在位內膜和非異位癥的在位內膜均有錶達,其錶達灰度值分彆為:0.5438±0.0565、0.3016±0.0614、0.1536±0.0444；HMGB1蛋白在三組內膜中錶達的灰度值分彆為:0.8399±0.0287、0.4010±0.0643、0.1999±0.0716.三組HMGB1 mRNA、HMGB1蛋白的錶達水平比較差異均有統計學意義(F=8.756,P＜0.05).HMGB1在各組不同月經時期錶達水平比較差異均無統計學意義(P＞0.05).卵巢子宮內膜異位癥患者臨床分期高級彆異位內膜中HMGB1錶達水平高于低級彆者(t=10.28,P＜0.01),與腫瘤大小、患者年齡無明顯相關(P＞0.05).結論 HMGB1在卵巢子宮內膜異位、在位內膜中呈高錶達,且臨床分期級彆越高其錶達增彊,其可能在卵巢子宮內膜異位癥的髮生、髮展中起著重要的作用,可作為子宮內膜異位癥的生物標記物.
목적 연구고천이솔족단백1(HMGB1)재란소자궁내막이위증중적표체,위자궁내막이위증적조기진단、치료급예후제공신적사로.방법 채용RT-PCR화western-blotting검측HMGB1 mRNA화HMGB1단백재69례란소자궁내막이위증환자이위자궁내막화재위내막급69례비자궁내막이위증환자적재위내막중(대조조)적표체정황.결과 HMGB1 mRNA재란소이위자궁내막、재위내막화비이위증적재위내막균유표체,기표체회도치분별위:0.5438±0.0565、0.3016±0.0614、0.1536±0.0444；HMGB1단백재삼조내막중표체적회도치분별위:0.8399±0.0287、0.4010±0.0643、0.1999±0.0716.삼조HMGB1 mRNA、HMGB1단백적표체수평비교차이균유통계학의의(F=8.756,P＜0.05).HMGB1재각조불동월경시기표체수평비교차이균무통계학의의(P＞0.05).란소자궁내막이위증환자림상분기고급별이위내막중HMGB1표체수평고우저급별자(t=10.28,P＜0.01),여종류대소、환자년령무명현상관(P＞0.05).결론 HMGB1재란소자궁내막이위、재위내막중정고표체,차림상분기급별월고기표체증강,기가능재란소자궁내막이위증적발생、발전중기착중요적작용,가작위자궁내막이위증적생물표기물.
Objective To investigate the expression of high mobility group protein box1 (HMGB1)in ovarian endometriosis tissue and to provide new idea for early diagnosis,treatment and prognosis of endometriosis.Methods The levels of HMGB1 in ectopic endometrium and eutopic endometrium of 69 patients with ovarian endometriosis and endometrium of 69 cases with non-endometrioses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western-blotting.Results HMGB1 was expressed in all the three groups (ectopic endometrium eutopic endometrium endometrium with non-endometrioses).The relative expression level of HMGB1 mRNA was 0.54383 ± 0.0565,0.3016 ± 0.0614,and 0.1536 ±0.0444,respectively (F =377.923,P ＜ 0.0001).The relative of HMGB1 protein was 0.83990 ±0.12869,0.40100 ±0.16425,and 0.19986 ± 0.08162,respectively (F =185.625,P =0.0000).The expression level of HMGB1 was related to clinical stages (t =10.28,P ＜0.01) ; however,it was not obviously related to age,the diameter of lump,and course of disease (P ＞ 0.05).Conclusions Over expression of HMGB1 in ovary endometriosis indicates that HMGB1 plays a significant role in occurrence and development of ovary endometriosis.The expression of HMGB1 in endometriosis eutopic endometrium was much higher than that of non-endometrioses endometrium,which supports the eutopic endometrium determinism.The overexpression of HMGB1 was consistent with clinical stage,which proves that HMGB1 maybe be tightly associated with the severity of the disease and to likely become biomarker.
