CAJ | 학술논문

目的检测汉坦病毒感染BALB/c小鼠组织中特异性抗原及病毒RNA,建立汉坦病毒感染动物的评价体系.方法将1000LD50的汉坦病毒悬液经肌肉注射感染BALB/c小鼠,在感染后的第3天,分别取小鼠的心、肝、脾、肺、肾、脑等组织研磨后制成病毒悬液,以ELISA法和qRT-PCR法分别检测各组织中的汉坦病毒特异性抗原及病毒RNA.结果 BALB/c小鼠感染汉坦病毒后短期内在脑和肝组织中可以检测到大量汉坦病毒特异性抗原以及病毒RNA,而心、脾、肺、肾组织中未检测到特异性抗原及病毒RNA.结论实验结果为建立汉坦病毒感染动物模型的评价体系提供了参考依据.
목적 검측한탄병독감염BALB/c소서조직중특이성항원급병독RNA,건립한탄병독감염동물적평개체계.방법 장1000LD50적한탄병독현액경기육주사감염BALB/c소서,재감염후적제3천,분별취소서적심、간、비、폐、신、뇌등조직연마후제성병독현액,이ELISA법화qRT-PCR법분별검측각조직중적한탄병독특이성항원급병독RNA.결과 BALB/c소서감염한탄병독후단기내재뇌화간조직중가이검측도대량한탄병독특이성항원이급병독RNA,이심、비、폐、신조직중미검측도특이성항원급병독RNA.결론 실험결과위건립한탄병독감염동물모형적평개체계제공료삼고의거.
Objective To establish an evaluation system about animals infected with hantavirus,an observation of the BALB/c mice infected with hantavirus was made.Methods BALB/c mice were infected with hantavirus by intramuscular injection with stock solution.The specific antigen from BALB/c mice tissues after 3 days was detected with enzyme-linked immunosorbent assay (ELISA) and viral RNA with real-time polymerase chain reaction (RT-PCR).Results Within a short term,the specific antigen and viral RNA were detected from the brain and liver at day 3 after infection,but not be detected from the heart,spleen,lung,and kidney samples.Conclusions The results provided ones with some information on animals infected with hantavirus.