中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
11期
1482-1484
,共3页
况丽平%詹晓芬%沈金辉%王少洪
況麗平%詹曉芬%瀋金輝%王少洪
황려평%첨효분%침금휘%왕소홍
胃肠道间质肿瘤/代谢%胃肠道间质肿瘤/病理学%抗原,CD34/生物合成%原癌基因蛋白质c-kit/生物合成
胃腸道間質腫瘤/代謝%胃腸道間質腫瘤/病理學%抗原,CD34/生物閤成%原癌基因蛋白質c-kit/生物閤成
위장도간질종류/대사%위장도간질종류/병이학%항원,CD34/생물합성%원암기인단백질c-kit/생물합성
Gastrointestinal stromal tumors/metabolism%Gastrointestinal stromal tumors/pathology%Antigens,CD34/biosynthesis%Proto-oncogene proteins c-kit/biosynthesis
目的 探讨DOG1、CD117及CD34在胃肠道间质瘤中的表达及病理诊断的意义.方法 应用免疫组化EnVision二步法,检测DOG1、CD117及CD34在60例胃肠道间质瘤(GIST)和14例其他类型间叶源性肿瘤组织中的表达.结果 GIST组织中DOG1蛋白表达明显高于非GIST间叶源性肿瘤(P =0.000).发生于胃的GSIT组织中DOG1蛋白表达明显高于肠道GIST(P=0.011).60例GIST组织中,DOG1、CD117和CD34蛋白阳性表达率分别为95％、95％和83.3％,DOG1蛋白表达高于CD34(P=0.040),而DOG1与CD117差异无统计学意义(P=1.000).CD117、CD34阳性表达GIST组织中DOG1表达率分别为94.7％、94.1％,CD117、CD34阴性表达GIST病例均全部表达DOG1(3/3,9/9),其中有3例CD117及CD34两者阴性,但DOG1阳性.结论 DOG1是一个诊断GIST具有较高敏感性和特异性的GIST标记物,在GIST临床病理诊断工作中,联合检测DOG1、CD117及CD34蛋白表达情况,有助于进一步提高诊断GIST的准确性.
目的 探討DOG1、CD117及CD34在胃腸道間質瘤中的錶達及病理診斷的意義.方法 應用免疫組化EnVision二步法,檢測DOG1、CD117及CD34在60例胃腸道間質瘤(GIST)和14例其他類型間葉源性腫瘤組織中的錶達.結果 GIST組織中DOG1蛋白錶達明顯高于非GIST間葉源性腫瘤(P =0.000).髮生于胃的GSIT組織中DOG1蛋白錶達明顯高于腸道GIST(P=0.011).60例GIST組織中,DOG1、CD117和CD34蛋白暘性錶達率分彆為95％、95％和83.3％,DOG1蛋白錶達高于CD34(P=0.040),而DOG1與CD117差異無統計學意義(P=1.000).CD117、CD34暘性錶達GIST組織中DOG1錶達率分彆為94.7％、94.1％,CD117、CD34陰性錶達GIST病例均全部錶達DOG1(3/3,9/9),其中有3例CD117及CD34兩者陰性,但DOG1暘性.結論 DOG1是一箇診斷GIST具有較高敏感性和特異性的GIST標記物,在GIST臨床病理診斷工作中,聯閤檢測DOG1、CD117及CD34蛋白錶達情況,有助于進一步提高診斷GIST的準確性.
목적 탐토DOG1、CD117급CD34재위장도간질류중적표체급병리진단적의의.방법 응용면역조화EnVision이보법,검측DOG1、CD117급CD34재60례위장도간질류(GIST)화14례기타류형간협원성종류조직중적표체.결과 GIST조직중DOG1단백표체명현고우비GIST간협원성종류(P =0.000).발생우위적GSIT조직중DOG1단백표체명현고우장도GIST(P=0.011).60례GIST조직중,DOG1、CD117화CD34단백양성표체솔분별위95％、95％화83.3％,DOG1단백표체고우CD34(P=0.040),이DOG1여CD117차이무통계학의의(P=1.000).CD117、CD34양성표체GIST조직중DOG1표체솔분별위94.7％、94.1％,CD117、CD34음성표체GIST병례균전부표체DOG1(3/3,9/9),기중유3례CD117급CD34량자음성,단DOG1양성.결론 DOG1시일개진단GIST구유교고민감성화특이성적GIST표기물,재GIST림상병리진단공작중,연합검측DOG1、CD117급CD34단백표체정황,유조우진일보제고진단GIST적준학성.
Objective To investigate the expression and diagnostic significance of DOG1,CD117and CD34 in gastrointestinal stromal tumors.Methods Expressions of DOG1,CD117 and CD34 were determined by immunohistochemical techniques (EnVision) in 60 cases of gastrointestinal stromal tumors (GIST) and 14 cases of other mesenchymal tumors.Results Expression of DOG1 in GIST was significantly higher than in other mesenchymal tumors (P =0.000).Expression of DOG1 in GIST from stomach was significantly higher than that from intestines.The expression rates of DOG1,CD117 and CD34 in GIST were 95％,95％,and 83.3％,respectively.Expression of DOG1 was significantly higher than CD34 (P =0.040),but no significant difference between DOG1 and CD117 (P =1.000).The expression rates of DOG1 in CD117-positive,CD34-positive tumors were 94.7％ and 94.1％,respectively.All the CD117-negative,CD34-negative tumors expressed DOG1,3 cases which were negative for CD117 and CD34 expressed DOG1.Conclusions DOG1 is a novel marker for GIST,It is necessary to the combined detection of the expression of DOG1,CD117 and CD34,which will further improve the diagnostic accuracy of GIST.
