中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
11期
1530-1532
,共3页
朱胜%石兰珍%傅广成%陈兰娟
硃勝%石蘭珍%傅廣成%陳蘭娟
주성%석란진%부엄성%진란연
供血者%肝炎病毒,乙型%抗体,病毒
供血者%肝炎病毒,乙型%抗體,病毒
공혈자%간염병독,을형%항체,병독
Blood donors%Hepatitis B virus%Antibodies,viral
目的 探讨无偿献血者乙型肝炎病毒核心抗体(anti-HBc)与隐匿性乙型肝炎病毒感染的关系.方法 收集无偿献血者样本9100份,采用ELISA法进行HBsAg和anti-HBc血清学筛查,anti-HBc阳性血清再通过PCR检测乙肝病毒核酸(HBV DNA).结果 9100份无偿献血者标本中anti-HBc阳性911份(10.01％),HBsAg阳性199份(2.19％);911份anti-HBc阳性标本中,HBsAg阴性820份(90.01％),HBV DNA阳性34份.结论 如果常规筛查不检测anti-HBc,血液中HBV DNA将被漏检.无偿献血者血液中HBsAg阴性的情况下,仍然存在病毒传播的潜在风险,有必要重视无偿献血者初筛HBsAg阴性人群的进一步血清学检测.
目的 探討無償獻血者乙型肝炎病毒覈心抗體(anti-HBc)與隱匿性乙型肝炎病毒感染的關繫.方法 收集無償獻血者樣本9100份,採用ELISA法進行HBsAg和anti-HBc血清學篩查,anti-HBc暘性血清再通過PCR檢測乙肝病毒覈痠(HBV DNA).結果 9100份無償獻血者標本中anti-HBc暘性911份(10.01％),HBsAg暘性199份(2.19％);911份anti-HBc暘性標本中,HBsAg陰性820份(90.01％),HBV DNA暘性34份.結論 如果常規篩查不檢測anti-HBc,血液中HBV DNA將被漏檢.無償獻血者血液中HBsAg陰性的情況下,仍然存在病毒傳播的潛在風險,有必要重視無償獻血者初篩HBsAg陰性人群的進一步血清學檢測.
목적 탐토무상헌혈자을형간염병독핵심항체(anti-HBc)여은닉성을형간염병독감염적관계.방법 수집무상헌혈자양본9100빈,채용ELISA법진행HBsAg화anti-HBc혈청학사사,anti-HBc양성혈청재통과PCR검측을간병독핵산(HBV DNA).결과 9100빈무상헌혈자표본중anti-HBc양성911빈(10.01％),HBsAg양성199빈(2.19％);911빈anti-HBc양성표본중,HBsAg음성820빈(90.01％),HBV DNA양성34빈.결론 여과상규사사불검측anti-HBc,혈액중HBV DNA장피루검.무상헌혈자혈액중HBsAg음성적정황하,잉연존재병독전파적잠재풍험,유필요중시무상헌혈자초사HBsAg음성인군적진일보혈청학검측.
Objective Some blood donors were negative for the surface antigen but positive for the core antibody,leading to acute hepatitis.This study was to determine the seroprevalence of the hepatitis B core antibody in voluntary blood donors.Methods A total of 9100 donor samples were screened for hepatitis B surface antigen and Hepatitis B core antibody with enzyme-linked immunosorbent assay(ELISA).The samples which were positive for the core antibody were subjected to real-time polymerase chain reaction (PCR) for the hepatitis B DNA detection.Results Among the 9100 donors,911 (10.01％) donors were positive for the core antibody.The hepatitis B surface antigen was positive in 199 (2.19％) donors.Among the 911 donors who were positive for the core antibody,820 (90.01％) donors were negative for the HB-sAg,and 35 donors were positive for hepatitis B DNA.Conclusions If a routine screening of the sera for the core antibody is not done,the HBV DNA viraemia may not be identified.The absence of the surface antigen in the blood of apparently healthy individuals may not be sufficient to ensure the lack of the circulating virus.It is necessary to attach importance to the blood donors screening HBsAg-negative population further serological testing,suspicious specimens should detect HBV DNA.
