CAJ | 학술논문

目的探讨多发性骨髓瘤(MM)患者骨髓间充质干细胞(BMMSC)体外生长特性和转化生长因子-β 1 (TGF-β 1)基因表达变化及意义.方法以7例MM患者为试验组(MM组),10例单纯缺铁性贫血患者为对照组,取骨髓单个核细胞进行BMMSC体外培养,观察BMMSC体外生长形态并计数,绘制生长曲线.取BMMSC提取总RNA,反转录-聚合酶链反应(RT-PCR)法测定两组BMMSC中TGF-β 1 mRNA表达水平.结果 MM患者BMMSC生长活性与对照组无明显差别；MM组BMMSC中TGF-β 1 mRNA表达水平(0.01241±0.00419)较对照组(0.00122±0.00030)升高,差异有统计学意义(t=3.218,P＜0.05).结论 MM患者BMMSC中TGF-β 1高表达可能与MM发病有关.
목적 탐토다발성골수류(MM)환자골수간충질간세포(BMMSC)체외생장특성화전화생장인자-β 1 (TGF-β 1)기인표체변화급의의.방법 이7례MM환자위시험조(MM조),10례단순결철성빈혈환자위대조조,취골수단개핵세포진행BMMSC체외배양,관찰BMMSC체외생장형태병계수,회제생장곡선.취BMMSC제취총RNA,반전록-취합매련반응(RT-PCR)법측정량조BMMSC중TGF-β 1 mRNA표체수평.결과 MM환자BMMSC생장활성여대조조무명현차별；MM조BMMSC중TGF-β 1 mRNA표체수평(0.01241±0.00419)교대조조(0.00122±0.00030)승고,차이유통계학의의(t=3.218,P＜0.05).결론 MM환자BMMSC중TGF-β 1고표체가능여MM발병유관.
Objective To investigate the expression and significance of transforming growth factor-β1 (TGF-β1) gene in bone marrow mesenchymal stem cell (BMMSC) derived from patients with multiple myeloma (MM).Methods BMMSC of 7 MM patients and 10 patients with iron deficiency anemia were cultured in vitro.The morphology of BMMSC was observed and the growth curve was portrayed according to the daily results of BMMSC proliferation.Total RNA was extracted from BMMSCs and transcription of TGF-β1 gene in BMMSC was measured by reverse transcription-PCR.Results The proliferative activity of BMMSC was not significantly different between the two groups,but expression of TGF-β1 gene of BMMSC was higher in MM patients (0.01241±0.00419) than the control group (0.00122±0.00030) (t =3.218,P ＜ 0.05).Conclusion The abnormally high expression of TGF-β31 gene in BMMSCs could contribute to the pathogenesis of MM.