CAJ | 학술논문

目的研究透毒复方青蒿鳖甲汤对急性髓系白血病完全缓解期(AML-CR)患者骨髓CD34+细胞来源树突状细胞(DC)生物学效应的影响.方法分离纯化骨髓CD34+细胞,体外不同浓度透毒中药含药血清与细胞因子联合诱导分化为DC,观察DC形态特征,流式细胞术检测DC表面分子CD80、CD83、CD86的表达,将DC分别与自体、异体外周血T细胞共同培养,四甲基偶氮唑蓝(MTT)比色法检测激活的T细胞不同效靶比对人类白血病细胞株(K562细胞)的杀伤效应.结果青蒿鳖甲汤联合细胞因子能促进AML-CR患者骨髓CD34+分化为形态典型DC,不同剂量含药血清均能上调DC表面特征性分子及共刺激分子CD80、CD83、CD86比例,较单纯细胞因子组比较差异有统计学意义(P＜0.01),且含药血清组较单纯细胞因子更能激发外周血T淋巴细胞对K562细胞的杀伤作用,两者间比较差异有统计学意义(P＜0.01).结论青蒿鳖甲汤能促进髓系微小残留白血病患者CD34+细胞向DC转化,提高DC的生物学效应.
목적 연구투독복방청호별갑탕대급성수계백혈병완전완해기(AML-CR)환자골수CD34+세포래원수돌상세포(DC)생물학효응적영향.방법 분리순화골수CD34+세포,체외불동농도투독중약함약혈청여세포인자연합유도분화위DC,관찰DC형태특정,류식세포술검측DC표면분자CD80、CD83、CD86적표체,장DC분별여자체、이체외주혈T세포공동배양,사갑기우담서람(MTT)비색법검측격활적T세포불동효파비대인류백혈병세포주(K562세포)적살상효응.결과 청호별갑탕연합세포인자능촉진AML-CR환자골수CD34+분화위형태전형DC,불동제량함약혈청균능상조DC표면특정성분자급공자격분자CD80、CD83、CD86비례,교단순세포인자조비교차이유통계학의의(P＜0.01),차함약혈청조교단순세포인자경능격발외주혈T림파세포대K562세포적살상작용,량자간비교차이유통계학의의(P＜0.01).결론 청호별갑탕능촉진수계미소잔류백혈병환자CD34+세포향DC전화,제고DC적생물학효응.
Objective To study the effect of the serum with modified preparation of Toudu TCM Qinghao Biejia Soup to the induction of dendritic cell (DC) of complete remission acute myeloid leukemia (AML-CR) patients with source of CD34+ cells.Methods CD34+ cells from AML-CR patients were isolated,and cultivated combined with serums of different concentrations of Qinghao Biejia Soup.Transformation was observed under inverted microscope for the morphological characteristics of DC.DC surface molecules of CD83,CD80,CD86 expression were detected by flow cytometry.MTT method was used to detect the killing effect of leukemia cell line K562 in different conditions,induced transformation mature DC incubated with foreign or autolous T cells respectively.Results Qinghao Biejia Soup promoted AML-CR CD34+ cells differente to DC with typical morphology.Mature DC with high expression of specific markers (CD80,CD83,CD86) (P ＜ 0.01).Conclusion Qinghao Biejia Soup can promote patients CD34+ cells to DC transformation.