CAJ | 학술논문

目的探讨非霍奇金淋巴瘤(NHL)患者外周血T细胞亚群及NK细胞检测的临床意义.方法收集病理确诊的初治NHL患者62例,采集静脉血经流式细胞术检测T细胞亚群及NK细胞水平,与30名健康对照者进行比较；并比较不同年龄、性别、病理类型患者之间的表达差异,分析其与临床分期、国际预后指数(IPI)、有无B症状等因素的相关性.结果 NHL组外周血CD3、CD4、NK细胞比例[(64.13±19.83)％、(27.10±8.20)％、(13.51±10.59)％]低于健康对照组[(65.78±10.69)％、(31.95±12.74)％、(18.76±7.36)％](P＜0.05)；CD8、调节性T细胞(Treg细胞)比例[(32.15±13.83)％、(10.44±3.00)％]高于健康对照组[(29.25±12.35)％、(7.51±4.36)％](P＜0.05).NHL患者≤60岁组CD3、NKT细胞比例[(67.06±19.24)％、(4.91±3.69)％]明显高于＞60岁组[(59.18±20.33)％、(4.89±3.05)％](P＜0.05),T细胞NHL组Treg细胞比例明显高于B细胞NHL组[(8.17±6.41)％比(7.11±2.53)％](P＜0.05).NHL患者外周血CD3、CD4、NK、NKT细胞比例与临床分期相关(均P＜ 0.05)；CD8细胞比例与IPI相关(P=0.000)；Treg细胞比例与IPI、B症状相关(均P＜ 0.05).结论 NHL患者存在细胞免疫抑制,其外周血T细胞亚群及NK细胞比例在不同性别、年龄、病理亚型之间存在不同,与临床分期、IPI、有无B症状等的相关性尚需进一步探讨.
목적 탐토비곽기금림파류(NHL)환자외주혈T세포아군급NK세포검측적림상의의.방법 수집병리학진적초치NHL환자62례,채집정맥혈경류식세포술검측T세포아군급NK세포수평,여30명건강대조자진행비교；병비교불동년령、성별、병리류형환자지간적표체차이,분석기여림상분기、국제예후지수(IPI)、유무B증상등인소적상관성.결과 NHL조외주혈CD3、CD4、NK세포비례[(64.13±19.83)％、(27.10±8.20)％、(13.51±10.59)％]저우건강대조조[(65.78±10.69)％、(31.95±12.74)％、(18.76±7.36)％](P＜0.05)；CD8、조절성T세포(Treg세포)비례[(32.15±13.83)％、(10.44±3.00)％]고우건강대조조[(29.25±12.35)％、(7.51±4.36)％](P＜0.05).NHL환자≤60세조CD3、NKT세포비례[(67.06±19.24)％、(4.91±3.69)％]명현고우＞60세조[(59.18±20.33)％、(4.89±3.05)％](P＜0.05),T세포NHL조Treg세포비례명현고우B세포NHL조[(8.17±6.41)％비(7.11±2.53)％](P＜0.05).NHL환자외주혈CD3、CD4、NK、NKT세포비례여림상분기상관(균P＜ 0.05)；CD8세포비례여IPI상관(P=0.000)；Treg세포비례여IPI、B증상상관(균P＜ 0.05).결론 NHL환자존재세포면역억제,기외주혈T세포아군급NK세포비례재불동성별、년령、병리아형지간존재불동,여림상분기、IPI、유무B증상등적상관성상수진일보탐토.
Objective To explore the clinical significance of T lymphocyte subsets and NK cells' detection in peripheral blood of patients with non-Hodgkin' s lymphoma (NHL).Methods 62 newly diagnosed NHL and 30 healthy adults were enrolled for analyzing.T lymphocyte subsets and NK cells were deteced by flow cytometry and compared between different groups according to age,sex and pathology.Multiple linear regression was used to analyze the correlation among the levels of T lymphocyte subsets and NK cells and several clinical factors,such as clinical stage,IPI and B symptoms.Results The proportions of CD3,CD4 and NK cells in peripheral blood of NHL group [(64.13±19.83) ％,(27.10±8.20) ％,(13.51± 10.59) ％] were significantly lower than those of normal control [(65.78±10.69) ％,(31.95±12.74) ％,(18.76± 7.36) ％] (P ＜ 0.05),while the proportions of CD8 and Treg cells in peripheral blood of NHL [(32.15±13.83) ％,(10.44±3.00) ％] were significantly higher than those of normal control [(29.25±12.35) ％,(7.51±4.36) ％] (P ＜ 0.05).In NHL,the proportions of CD3 and NKT cells [(67.06±19.24) ％,(4.91±3.69) ％] in ≤60 years old group were significantly higher than those in ＞ 60 years old group [(59.18±20.33) ％,(4.89±3.05) ％] (P ＜ 0.05).The proportions of Treg cells in T-NHL was significantly higher than that in B-NHL [(8.17±6.41) ％ vs (7.11±2.53) ％] (P ＜ 0.05).The proportions of CD3,CD4,NK and NKT cells in peripheral blood of NHL were significantly correlated with clinical stage (P ＜ 0.05),that of CD8 cells were significantly correlated with IPI (P =0.000),that of Treg cells were significantly correlated with IPI and B symptoms (P ＜ 0.05).Conclusion The cellular immune is suppressed in NHL patients.The proportions of T lymphocyte subsets and NK cells in peripheral blood of patients with NHL are different between different groups according to age,sex and pathology.The correlations among that and clinical factors need to be further explored.