白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2014年
1期
30-32,37
,共4页
陈姣%王晓冬%万纯黔%钟凌
陳姣%王曉鼕%萬純黔%鐘凌
진교%왕효동%만순검%종릉
多发性骨髓瘤%Hepcidin-25%治疗结果%预后
多髮性骨髓瘤%Hepcidin-25%治療結果%預後
다발성골수류%Hepcidin-25%치료결과%예후
Multiple myeloma%Hepcidin-25%Treatment outcome%Prognosis
目的 探讨Hepcidin-25在多发性骨髓瘤(MM)患者不同疾病过程中的动态变化及其与疗效、预后的关系.方法 在临床诊疗的不同阶段分别采用实时定量聚合酶链反应(RT-PCR)、酶联免疫吸附法(ELISA)分析54例MM患者Hepcidin-25的mRNA及蛋白表达水平,研究Hepcidin-25的动态变化与患者临床表现的相关性及其对患者预后的临床预测价值.结果 MM患者Hepcidin-25 mRNA表达量明显高于健康对照组(0.58±0.19比0.08±0.03,P=0.001),其蛋白质水平也明显高于健康对照组[(5.2±11.9)ng/ml比(22.5±7.1)ng/ml,P=0.019)].对MM患者随访发现,治疗后缓解患者(17例)的Hepcidin-25水平为(26.4±7.3) ng/ml,较其治疗前的(33.4±7.4) ng/ ml降低(t=5.312,P=0.021).而治疗无效病情加重患者(37例)的Hepcidin-25水平为(55.9±12.7)ng/ml,较治疗前的(39.1 ±9.9)ng/ml增高(t=2.811,P=0.037).治疗缓解组与治疗无效病情加重组患者生存率差异有统计学意义(P＜0.01).治疗后缓解患者的血红蛋白(Hh)水平为124g/L,较治疗前的102 g/L升高(t=2.113,P=0.035),与Hepcidin-25具有很好的相关性(r=-0.535,P=0.002).而治疗无效病情加重患者的Hb水平为46 g/L,较治疗前的73 g/L降低(t=2.730,P=0.036),与Hepcidin-25呈负相关(r=-0.642,P=0.001).结论 MM患者的Hepcidin-25表达量较高,与慢性病贫血密切相关.较高水平的Hepcidin-25表达量对临床复发有一定的预测价值,与MM患者疗效、预后有密切关系.
目的 探討Hepcidin-25在多髮性骨髓瘤(MM)患者不同疾病過程中的動態變化及其與療效、預後的關繫.方法 在臨床診療的不同階段分彆採用實時定量聚閤酶鏈反應(RT-PCR)、酶聯免疫吸附法(ELISA)分析54例MM患者Hepcidin-25的mRNA及蛋白錶達水平,研究Hepcidin-25的動態變化與患者臨床錶現的相關性及其對患者預後的臨床預測價值.結果 MM患者Hepcidin-25 mRNA錶達量明顯高于健康對照組(0.58±0.19比0.08±0.03,P=0.001),其蛋白質水平也明顯高于健康對照組[(5.2±11.9)ng/ml比(22.5±7.1)ng/ml,P=0.019)].對MM患者隨訪髮現,治療後緩解患者(17例)的Hepcidin-25水平為(26.4±7.3) ng/ml,較其治療前的(33.4±7.4) ng/ ml降低(t=5.312,P=0.021).而治療無效病情加重患者(37例)的Hepcidin-25水平為(55.9±12.7)ng/ml,較治療前的(39.1 ±9.9)ng/ml增高(t=2.811,P=0.037).治療緩解組與治療無效病情加重組患者生存率差異有統計學意義(P＜0.01).治療後緩解患者的血紅蛋白(Hh)水平為124g/L,較治療前的102 g/L升高(t=2.113,P=0.035),與Hepcidin-25具有很好的相關性(r=-0.535,P=0.002).而治療無效病情加重患者的Hb水平為46 g/L,較治療前的73 g/L降低(t=2.730,P=0.036),與Hepcidin-25呈負相關(r=-0.642,P=0.001).結論 MM患者的Hepcidin-25錶達量較高,與慢性病貧血密切相關.較高水平的Hepcidin-25錶達量對臨床複髮有一定的預測價值,與MM患者療效、預後有密切關繫.
목적 탐토Hepcidin-25재다발성골수류(MM)환자불동질병과정중적동태변화급기여료효、예후적관계.방법 재림상진료적불동계단분별채용실시정량취합매련반응(RT-PCR)、매련면역흡부법(ELISA)분석54례MM환자Hepcidin-25적mRNA급단백표체수평,연구Hepcidin-25적동태변화여환자림상표현적상관성급기대환자예후적림상예측개치.결과 MM환자Hepcidin-25 mRNA표체량명현고우건강대조조(0.58±0.19비0.08±0.03,P=0.001),기단백질수평야명현고우건강대조조[(5.2±11.9)ng/ml비(22.5±7.1)ng/ml,P=0.019)].대MM환자수방발현,치료후완해환자(17례)적Hepcidin-25수평위(26.4±7.3) ng/ml,교기치료전적(33.4±7.4) ng/ ml강저(t=5.312,P=0.021).이치료무효병정가중환자(37례)적Hepcidin-25수평위(55.9±12.7)ng/ml,교치료전적(39.1 ±9.9)ng/ml증고(t=2.811,P=0.037).치료완해조여치료무효병정가중조환자생존솔차이유통계학의의(P＜0.01).치료후완해환자적혈홍단백(Hh)수평위124g/L,교치료전적102 g/L승고(t=2.113,P=0.035),여Hepcidin-25구유흔호적상관성(r=-0.535,P=0.002).이치료무효병정가중환자적Hb수평위46 g/L,교치료전적73 g/L강저(t=2.730,P=0.036),여Hepcidin-25정부상관(r=-0.642,P=0.001).결론 MM환자적Hepcidin-25표체량교고,여만성병빈혈밀절상관.교고수평적Hepcidin-25표체량대림상복발유일정적예측개치,여MM환자료효、예후유밀절관계.
Objective To study the dynamic changes of Hepcidin-25 in different disease process in patients with multiple myeloma (MM) and its relationship with therapeutic effect and prognosis.Methods The expression levels of Hepcidin-25 in 54 MM patients were analyzed by RT-PCR and ELISA.The correlations between dynamic changes of patients' Hepcidin-25 expression and clinical manifestations,clinical predictive value of the prognosis were researched.Results Expressions of Hepcidin-25 mRNA and protein in MM patients were significantly higher than those in normal control group [0.58±0.19 vs 0.08±0.027,P =0.001,(5.2±11.9) ng/ml vs (22.5±7.1) ng/ml,P =0.019].Hepcidin-25 expression of 17 patients remission after treatment was (26.4±7.3) ng/ml,it was significantly lower than that before treatment [(33.4±7.4) ng/ml,t =5.312,P =0.021].But the Hepcidin-25 level of the treatment ineffective patients (37 cases) significantly increased [(55.9±12.7) ng/ml vs (39.1±9.9) ng/ml,t =2.811,P =0.037].There existed obvious differences in survival curves of two groups (P ＜ 0.01).Hb level of patients remission after treatment was 124 g/L,and significantly higher than that before treatment (102 g/L,t =2.113,P =0.035).It had a negative correlation with Hepcidin-25 (r =-0.535,P =0.002).But the Hepcidin-25 level of the treatment ineffective patients significantly increased (46 g/L vs 73 g/L,t =2.730,P =0.036).It also had a negative correlation with Hepcidin-25 (r =-0.642,P =0.001).Conclusions Hepcidin-25 expression levels in patients with MM are high,and are closely related to anemia of chronic disease.High expression of Hepcidin-25 has a predictive value for clinical effect and prognosis.
