CAJ | 학술논문

目的分析侵袭性NK细胞白血病的临床特征及诊治方法,探讨流式细胞术(FCM)对其诊断的价值.方法分析1例侵袭性NK细胞白血病患者的临床特征,并进行文献复习.结果患者持续高热、肝脾进行性增大、血三系细胞减少,骨髓中可见不典型细胞,FCM检查示骨髓中NK细胞约占淋巴细胞的83.3％,免疫表型为CD34-、CD2+、CD7+、CD3-、CyCD3+、CD5-、CD16+、CD56+、CD30-、CD4-、CD8-、CD117-、CD11c-、CD19、CD45++、SSC+-++,TCR、IgH基因重排阴性,染色体正常,诊断为侵袭性NK细胞白血病.结论侵袭性NK细胞白血病是一种少见的血液系统恶性疾病,临床表现多样、疾病进展迅速,早期容易误诊,FCM免疫分型结合骨髓细胞学涂片具有简便、快捷、可行、创伤性小的优势,在一些特殊情况下可作为首选检测手段.
목적 분석침습성NK세포백혈병적림상특정급진치방법,탐토류식세포술(FCM)대기진단적개치.방법 분석1례침습성NK세포백혈병환자적림상특정,병진행문헌복습.결과 환자지속고열、간비진행성증대、혈삼계세포감소,골수중가견불전형세포,FCM검사시골수중NK세포약점림파세포적83.3％,면역표형위CD34-、CD2+、CD7+、CD3-、CyCD3+、CD5-、CD16+、CD56+、CD30-、CD4-、CD8-、CD117-、CD11c-、CD19、CD45++、SSC+-++,TCR、IgH기인중배음성,염색체정상,진단위침습성NK세포백혈병.결론 침습성NK세포백혈병시일충소견적혈액계통악성질병,림상표현다양、질병진전신속,조기용역오진,FCM면역분형결합골수세포학도편구유간편、쾌첩、가행、창상성소적우세,재일사특수정황하가작위수선검측수단.
Objective To explore clinical characteristics and diagnosis of aggressive natural killercell leukemia (ANKL),and evaluate the value of flow cytometry (FCM) in diagnosing it.Methods A case of ANKL was reported and literatures were reviewed.Results The patient presented with persistent high fever,progressive pancytopenia and hepatosplenomegaly.The untypical cells could be seen by morphology.By FCM,NK cells consisted 83.3 ％ of total lymphocytes in bone marrow and immunophenotypes were CD34-,CD2+,CD7+,CD3-,CyCD3+,CD5-,CD16+,CD56+,CD30-,CD4-,CD8-,CD117-,CD11c,CD19-,CD45++,SSC+-++.T-cell receptor (TCR) and IgH gene rearrangement were negative and chromosome was normal.The patient was diagonised ANKL eventually.Conclusions ANKL is a quite rare disease with highly aggressive,poor prognosis and could be misdiagnosed easily.FCM combined with morphology is a convenient,handy,practicable and less invasive device for the diagnosis,and can be a preferred detection technique in some cases.