CAJ | 학술논문

目的检测成年T细胞急性淋巴细胞白血病(T-ALL)患者Ikaros6的表达,并探讨其临床意义.方法提取成年T-ALL患者初发时骨髓单个核细胞的RNA,反转录为cDNA,采用反转录-聚合酶链反应(RT-PCR)扩增技术进行检测,测序进一步确定结果,并结合患者临床资料分析临床特点及其预后.结果 74例初发成年人T-ALL中,Ikaros6阳性率为21.6％(16例),另外阳性患者较阴性患者更易发生髓外浸润(x2=4.084,P=0.043),且外周血白细胞计数高(103.15×109/L比15.60×109/L)、贫血更严重(血红蛋白75.95 g/L比107.00 g/L)和血小板计数低(26.0×109/L比67.0×109/L),但差异均无统计学意义(均P＞ 0.05).Ikaros6阳性患者生存期短和具有高复发风险.结论 Ikaros6在成年人T-ALL中表达率较高,阳性患者具有外周血白细胞计数高和易出现髓外浸润等临床特点,预后差,具有高复发风险.Ikaros6可考虑作为成年人T-ALL分层治疗或预后判断的分子指标.
목적 검측성년T세포급성림파세포백혈병(T-ALL)환자Ikaros6적표체,병탐토기림상의의.방법 제취성년T-ALL환자초발시골수단개핵세포적RNA,반전록위cDNA,채용반전록-취합매련반응(RT-PCR)확증기술진행검측,측서진일보학정결과,병결합환자림상자료분석림상특점급기예후.결과 74례초발성년인T-ALL중,Ikaros6양성솔위21.6％(16례),령외양성환자교음성환자경역발생수외침윤(x2=4.084,P=0.043),차외주혈백세포계수고(103.15×109/L비15.60×109/L)、빈혈경엄중(혈홍단백75.95 g/L비107.00 g/L)화혈소판계수저(26.0×109/L비67.0×109/L),단차이균무통계학의의(균P＞ 0.05).Ikaros6양성환자생존기단화구유고복발풍험.결론 Ikaros6재성년인T-ALL중표체솔교고,양성환자구유외주혈백세포계수고화역출현수외침윤등림상특점,예후차,구유고복발풍험.Ikaros6가고필작위성년인T-ALL분층치료혹예후판단적분자지표.
Objective To investigate the expression and prognostic value of Ikaros6 in adult T-cell acute lymphoblastic leukemia(T-ALL) patients.Methods The RNA was extracted from mononuclear cells of bone marrow in 74 adult T-ALL patients.The expression of Ikaros6 was examined by reverse transcription-PCR,and the results were confirmed by sequencing.Clinical features and prognosis were analyzed based on clinical data.Results In 74 T-ALL patients,the incidence rate of Ikaros6 was 21.6 ％ (16/74).Extramedullary infiltrations were occurred often (x2 =4.084,P =0.043),had higher WBC (103.15 × 109/L vs 15.60×109/L,t =0.214,P =0.831),more severe anemia (75.95 g/L vs 107.00 g/L,t =1.504,P =0.142) and lower platelet count (26.0×109/L vs 67.0×109/L,t =1.421,P =0.164) in patients with Ikaros6 positive.Meanwhile Ikaros6-positive patients had inferior survival and were increased risk of relapse as compared with the Ikaros6-negative patients.Conclusions The incidence of Ikaros6 is high in adult T-ALL patients.Ikaros6-positive patients are more likely to have extramedullary infiltration and higher WBC,meanwhile they had inferior survival and increased risk of relapse.Thus Ikaros6 may be served as a valuable factor for risk stratification and prognosis evaluation in adult T-ALL patients.