CAJ | 학술논문

目的探讨细针穿刺(FNA)细胞学(FNAC)联合流式细胞术(FCM)在B细胞非霍奇金淋巴瘤(B-NHL)诊断及分类中的价值.方法对17例经组织病理诊断为B-NHL的患者行FNA,获取穿刺细胞后迅速涂片,经瑞特-吉姆萨染色及显微镜观察细胞形态;同时,制备细胞悬液,标记相应抗体后在流式细胞仪上检测,结果与组织病理诊断比较.结果 17例B-NHL患者中,FNAC联合FCM可诊断15例,l例因取材不佳无法诊断,另1例考虑为反应性淋巴组织增生,与病理诊断符合率为93.8％;进一步分类诊断符合率为93.3％.结论在临床高度怀疑为淋巴瘤但无法行病理活组织检查的特殊情况下,FNAC联合FCM可为B-NHL的诊断及分类提供重要的信息.
목적 탐토세침천자(FNA)세포학(FNAC)연합류식세포술(FCM)재B세포비곽기금림파류(B-NHL)진단급분류중적개치.방법 대17례경조직병리진단위B-NHL적환자행FNA,획취천자세포후신속도편,경서특-길모살염색급현미경관찰세포형태;동시,제비세포현액,표기상응항체후재류식세포의상검측,결과 여조직병리진단비교.결과 17례B-NHL환자중,FNAC연합FCM가진단15례,l례인취재불가무법진단,령1례고필위반응성림파조직증생,여병리진단부합솔위93.8％;진일보분류진단부합솔위93.3％.결론 재림상고도부의위림파류단무법행병리활조직검사적특수정황하,FNAC연합FCM가위B-NHL적진단급분류제공중요적신식.
Objective To evaluate the accuracy of fine needle aspiration (FNA) cytology (FNAC)combined with flow cytometry (FCM) in diagnosing and classifying B-cell non-Hodgkin lymphoma (B-NHL).Methods FNA was performed on 17 patients with histopathology-diagnosed lymphoma.Some of FNA material was smeared on slides and stained by Wright' s stain,and then cell morphology was observed under microscope.The left material was prepared to cell suspensions and tested for immunophenotype with flow cytometry.According to the 2008 WHO NHL classification,the B-NHL was diagnosed and classified through FNAC combined with FCM.The results were compared with histopathology results.Results In 17 cases of B-NHL which were diagnosed by histopathology,15 cases were diagnosed by FNAC combined with FCM.Among the two negative cases,one was due to insufficient material and the other was diagnosed as reactive lymphoid tissue hyperplasia.So,the coincidence rates of diagnose and classification were 93.8 ％ and 93.3 ％.Conclusion FNAC combined with FCM may afford sufficient evidence to diagnose and classify B-NHL,especially for those cases that are highly suspicious for lymphoma but difficult to get histopathology.