白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2014年
6期
334-338
,共5页
李慧%刘美%黎红佳%黎伟%谢文瑾
李慧%劉美%黎紅佳%黎偉%謝文瑾
리혜%류미%려홍가%려위%사문근
白血病%丹参酮ⅡA%细胞凋亡%细胞周期%半胱氨酸天冬氨酸蛋白酶3
白血病%丹參酮ⅡA%細胞凋亡%細胞週期%半胱氨痠天鼕氨痠蛋白酶3
백혈병%단삼동ⅡA%세포조망%세포주기%반광안산천동안산단백매3
Leukemia%Tanshinone ⅡA%Apoptosis%Cell cycle%Caspase 3
目的 探讨丹参酮ⅡA(TanⅡA)对髓系白血病细胞株NB4、K562、THP-1增殖抑制作用及促凋亡作用.方法 将不同浓度TanⅡA与NB4、K562、THP-1细胞共培养24、48、72 h,以柔红霉素为阳性对照,采用四甲基偶氮唑蓝(MTT)法检测TanⅡA对白血病细胞株的增殖抑制作用,AnnexinV-FITC/PI流式细胞术检测细胞凋亡和细胞周期,紫外分光光度法检测Caspase-3蛋白表达.结果 TanⅡA作用24、48、72 h对NB4细胞的IC50值分别为24.11、9.60、7.28 μmol/L,对K562细胞IC50值分别为31.75、11.88、6.81 μmol/L,对THP-1细胞IC50值分别为111.10、32.82、11.82 μmol/L.流式细胞术检测结果显示:与空白对照组相比,TanⅡA与各白血病细胞株作用48 h后,细胞凋亡明显增加,G1期细胞数增加,Caspase-3蛋白表达显著升高(P＜0.05).结论 TanⅡA对白血病细胞具有增殖抑制与促凋亡作用,其作用强度从高到低依次为NB4、K562、THP-1细胞.其抗癌作用机制可能与阻滞细胞于G1期,上调Caspase-3表达有关.
目的 探討丹參酮ⅡA(TanⅡA)對髓繫白血病細胞株NB4、K562、THP-1增殖抑製作用及促凋亡作用.方法 將不同濃度TanⅡA與NB4、K562、THP-1細胞共培養24、48、72 h,以柔紅黴素為暘性對照,採用四甲基偶氮唑藍(MTT)法檢測TanⅡA對白血病細胞株的增殖抑製作用,AnnexinV-FITC/PI流式細胞術檢測細胞凋亡和細胞週期,紫外分光光度法檢測Caspase-3蛋白錶達.結果 TanⅡA作用24、48、72 h對NB4細胞的IC50值分彆為24.11、9.60、7.28 μmol/L,對K562細胞IC50值分彆為31.75、11.88、6.81 μmol/L,對THP-1細胞IC50值分彆為111.10、32.82、11.82 μmol/L.流式細胞術檢測結果顯示:與空白對照組相比,TanⅡA與各白血病細胞株作用48 h後,細胞凋亡明顯增加,G1期細胞數增加,Caspase-3蛋白錶達顯著升高(P＜0.05).結論 TanⅡA對白血病細胞具有增殖抑製與促凋亡作用,其作用彊度從高到低依次為NB4、K562、THP-1細胞.其抗癌作用機製可能與阻滯細胞于G1期,上調Caspase-3錶達有關.
목적 탐토단삼동ⅡA(TanⅡA)대수계백혈병세포주NB4、K562、THP-1증식억제작용급촉조망작용.방법 장불동농도TanⅡA여NB4、K562、THP-1세포공배양24、48、72 h,이유홍매소위양성대조,채용사갑기우담서람(MTT)법검측TanⅡA대백혈병세포주적증식억제작용,AnnexinV-FITC/PI류식세포술검측세포조망화세포주기,자외분광광도법검측Caspase-3단백표체.결과 TanⅡA작용24、48、72 h대NB4세포적IC50치분별위24.11、9.60、7.28 μmol/L,대K562세포IC50치분별위31.75、11.88、6.81 μmol/L,대THP-1세포IC50치분별위111.10、32.82、11.82 μmol/L.류식세포술검측결과현시:여공백대조조상비,TanⅡA여각백혈병세포주작용48 h후,세포조망명현증가,G1기세포수증가,Caspase-3단백표체현저승고(P＜0.05).결론 TanⅡA대백혈병세포구유증식억제여촉조망작용,기작용강도종고도저의차위NB4、K562、THP-1세포.기항암작용궤제가능여조체세포우G1기,상조Caspase-3표체유관.
Objective To investigate effects of Tanshinone Ⅱ A (Tan Ⅱ) on proliferation and apoptosis of myeloblastic leukemia cell lines.Methods NB4,K562 and THP-1 cells were incubated with TanⅡA for 24,48 and 72 hours.Ddaunorubicin was used as a positive control.Cell proliferation was monitored by MTT assay.Cell apoptosis and cell cycle were determined by Annexin Ⅴ-FITC/PI flow cytometry.Expression of Caspase-3 was quantified by spectrophotometry.Results After incubation with various leukemia cells for 24,48 and 72 hours,Tan Ⅱ inhibited proliferation of NB4 cells with IC50 of 24.11,9.60 and 7.28 μmol/L,inhibited K562 cells with IC50 of 31.75,11.88 and 6.81 μmol/L and inhibited THP-1 cells with IC50 of 111.10,32.82 and 11.82,respectively.After treatment with Tan Ⅱ for 48 hours,cell apoptosis,the number of G1 phase cells and the expression of Caspase-3 in all three leukemia cell lines were increased significantly comparing with the blank control group (P ＜ 0.05).Conclusions Tan Ⅱ A has proliferation inhibitory effect on myeloblastic leukemia cell lines by the order of effect NB4＞K562＞THP-1.Tan ⅡA displays anti-leukemia activity possibly through arresting leukemia cells in G1 phase and inducing apoptosis by increasing Caspase-3 expression.
