白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2014年
6期
350-353
,共4页
董华丽%吕楠%李大启%陈萍%邵建华%董学斌
董華麗%呂楠%李大啟%陳萍%邵建華%董學斌
동화려%려남%리대계%진평%소건화%동학빈
淋巴瘤,非霍奇金%垂体瘤转化基因%基因,myc%基因表达
淋巴瘤,非霍奇金%垂體瘤轉化基因%基因,myc%基因錶達
림파류,비곽기금%수체류전화기인%기인,myc%기인표체
Lymphoma,non-Hodgkin%Pituitary tumor transforming gene%Genes,myc%Gene expression
目的 探讨非霍奇金淋巴瘤(NHL)患者骨髓垂体瘤转化基因(PTTG)与c-myc基因的表达及其临床意义.方法 采用反转录聚合酶链反应(RT-PCR)法检测38例NHL患者骨髓及10例慢性淋巴结炎患者骨髓单个核细胞(BMMNC)中PTTG及c-myc基因的表达.结果 NHL患者骨髓中PTTG和c-myc基因的表达水平均明显高于慢性淋巴结炎组,差异有统计学意义(PTTG:0.567 7±0.270 7比0.071 2±0.020 1,t=4.706,P＜0.05; c-myc:0.352 6±0.185 4比0.107 3±0.043 5,t=3.303,P=0.002).外周T细胞淋巴瘤和弥漫大B细胞淋巴瘤患者PTTG及c-myc基因表达水平差异均无统计学意义(PTTG:0.556 8±0.211 3比0.602 8±0.244 6,t=0.640,P=0.527;c-myc:0.350 1±0.177 6比0.361 0±0.190 2,t=0.302,P=0.765).PTTG基因的表达与NHL骨髓浸润、国际预后指数(IPI)评分呈正相关(r=0.422,r=0.387;均P＜0.05).c-myc基因的表达与NHL骨髓浸润、IPI评分呈正相关(r=0.431,r=0.344,均P＜0.05).NHL患者骨髓PTTG基因和c-myc基因的表达呈正相关(r=0.490,P＜0.05).结论 NHL中PTTG、c-myc呈高表达状态,PTTG可能通过直接或间接途径促进c-myc的高表达.
目的 探討非霍奇金淋巴瘤(NHL)患者骨髓垂體瘤轉化基因(PTTG)與c-myc基因的錶達及其臨床意義.方法 採用反轉錄聚閤酶鏈反應(RT-PCR)法檢測38例NHL患者骨髓及10例慢性淋巴結炎患者骨髓單箇覈細胞(BMMNC)中PTTG及c-myc基因的錶達.結果 NHL患者骨髓中PTTG和c-myc基因的錶達水平均明顯高于慢性淋巴結炎組,差異有統計學意義(PTTG:0.567 7±0.270 7比0.071 2±0.020 1,t=4.706,P＜0.05; c-myc:0.352 6±0.185 4比0.107 3±0.043 5,t=3.303,P=0.002).外週T細胞淋巴瘤和瀰漫大B細胞淋巴瘤患者PTTG及c-myc基因錶達水平差異均無統計學意義(PTTG:0.556 8±0.211 3比0.602 8±0.244 6,t=0.640,P=0.527;c-myc:0.350 1±0.177 6比0.361 0±0.190 2,t=0.302,P=0.765).PTTG基因的錶達與NHL骨髓浸潤、國際預後指數(IPI)評分呈正相關(r=0.422,r=0.387;均P＜0.05).c-myc基因的錶達與NHL骨髓浸潤、IPI評分呈正相關(r=0.431,r=0.344,均P＜0.05).NHL患者骨髓PTTG基因和c-myc基因的錶達呈正相關(r=0.490,P＜0.05).結論 NHL中PTTG、c-myc呈高錶達狀態,PTTG可能通過直接或間接途徑促進c-myc的高錶達.
목적 탐토비곽기금림파류(NHL)환자골수수체류전화기인(PTTG)여c-myc기인적표체급기림상의의.방법 채용반전록취합매련반응(RT-PCR)법검측38례NHL환자골수급10례만성림파결염환자골수단개핵세포(BMMNC)중PTTG급c-myc기인적표체.결과 NHL환자골수중PTTG화c-myc기인적표체수평균명현고우만성림파결염조,차이유통계학의의(PTTG:0.567 7±0.270 7비0.071 2±0.020 1,t=4.706,P＜0.05; c-myc:0.352 6±0.185 4비0.107 3±0.043 5,t=3.303,P=0.002).외주T세포림파류화미만대B세포림파류환자PTTG급c-myc기인표체수평차이균무통계학의의(PTTG:0.556 8±0.211 3비0.602 8±0.244 6,t=0.640,P=0.527;c-myc:0.350 1±0.177 6비0.361 0±0.190 2,t=0.302,P=0.765).PTTG기인적표체여NHL골수침윤、국제예후지수(IPI)평분정정상관(r=0.422,r=0.387;균P＜0.05).c-myc기인적표체여NHL골수침윤、IPI평분정정상관(r=0.431,r=0.344,균P＜0.05).NHL환자골수PTTG기인화c-myc기인적표체정정상관(r=0.490,P＜0.05).결론 NHL중PTTG、c-myc정고표체상태,PTTG가능통과직접혹간접도경촉진c-myc적고표체.
Objective To explore the expression of pituitary tumor transforming gene (PTTG) and c-myc gene in patients with non-Hodgkin lymphoma (NHL),its relationship with NHL and its clinical significance.Methods PTTG mRNA and c-myc mRNA levels in bone marrow mononuclear cell (BMMNC) isolated from 38 NHL patients and 10 chronic lymphadenitis patients were quantified by reverse transcriptionpolymerase chain reaction (RT-PCR).Results mRNA expression of PTTG and c-myc gene in BMMNC was significantly higher in NHL patients than those in normal controls (PTTG:0.567 7±0.270 7 vs 0.071 2± 0.020 1,t =4.706,P ＜ 0.05; c-myc:0.352 6±0.185 4 vs 0.107 3±0.043 5,t =3.303,P =0.002).The expression of PTTG and c-myc gene in peripheral T cell lymphoma and diffuse large B cell lymphoma showed no significant difference (PTTG:0.556 8±0.211 3 vs 0.602 8±0.244 6,t =0.640,P =0.527; c-myc:0.350 1± 0.177 6 vs 0.361 0±0.190 2,t =0.302,P =0.765).Both expression of PTTG and c-myc were positively related to NHL clinical stage and IPI.Expression of PTTG mRNA was positively related to the expression of c-myc mRNA.Conclusion There was overexpression of PTTG and c-myc in NHL,which indicates that PTTG might be involved in tumorigenesis of NHL through direct or indirect activation of c-myc oncogene.
