CAJ | 학술논문

目的研究伯基特淋巴瘤(BL)中差异表达的miRNA,探讨可用于BL鉴别诊断的miRNA标志物.方法收集BL病例,miRNA芯片筛选其与淋巴结反应性增生之间的差异miRNA表达谱;运用miRWalk数据库对差异miRNA进行靶基因预测;利用MAS3对靶基因进行GO注释和KEGG信号通路分析.结果与淋巴结反应性增生组织相比,BL中共有46个表达异常的miRNA,包括3 1个上调miRNA和15个下调miRNA;对miRNA let-7f-1-3p靶基因预测得到2837条靶基因,与BL密切相关的基因有16个:CENPC1、FANCF、IL4R等;GO注释显示主要涉及多细胞器官发育,RNA聚合酶Ⅱ启动子转录调控等生物学过程,KEGG分析显示主要涉及癌症信号通路,TGF-beta信号通路中.结论在BL中发现了表达异常的miRNA,生物信息学分析对差异miRNA分析结果提示,这些差异miRNA可以作为BL鉴别诊断的新肿瘤标志物.
목적 연구백기특림파류(BL)중차이표체적miRNA,탐토가용우BL감별진단적miRNA표지물.방법 수집BL병례,miRNA심편사선기여림파결반응성증생지간적차이miRNA표체보;운용miRWalk수거고대차이miRNA진행파기인예측;이용MAS3대파기인진행GO주석화KEGG신호통로분석.결과 여림파결반응성증생조직상비,BL중공유46개표체이상적miRNA,포괄3 1개상조miRNA화15개하조miRNA;대miRNA let-7f-1-3p파기인예측득도2837조파기인,여BL밀절상관적기인유16개:CENPC1、FANCF、IL4R등;GO주석현시주요섭급다세포기관발육,RNA취합매Ⅱ계동자전록조공등생물학과정,KEGG분석현시주요섭급암증신호통로,TGF-beta신호통로중.결론 재BL중발현료표체이상적miRNA,생물신식학분석대차이miRNA분석결과제시,저사차이miRNA가이작위BL감별진단적신종류표지물.
Objective To explore the differential expression of microRNAs (miRNAs) in Burkitt lymphoma (BL) and their potential use as biomarkers for BL diagnosis.Methods The different miRNAs in BL from reactive hyperplasia of lymph node cases were screened by miRNA chip.The potential targets of miRNAs were predicted using miRWALK.MAS3 program was used to determine the putative functions of potential miRNA target genes by annotation using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses.Results A total of 46 miRNAs (36 upregulated and 21 downregulated) were dysregulated in BL compared with reactive hyperplasia of lymph node.Interestingly,members of the let-7 family (let-7f-1-3p) was downregulated in BL.The target genes of let-7f-1-3p were predicted,and the GO enrichment analysis revealed their functions were mainly related with multicellular organismal development and regulation of transcription from RNA polymerase Ⅱ promote.KEGG pathway analysis was also carried out among the predicted target genes,which showed that they were mainly involved in TGF-beta signaling pathway and related with chronic myeloid leukemia.Conclusions The set of differentially expressed miRNAs identified here expands the range of potential diagnostic markers for BL.