CAJ | 학술논문

目的探讨结核杆菌复苏因子(TB-RPF)在结核患者标本的分枝杆菌培养中的作用.方法收集疑似结核患者的痰液42例,支冲液8例,胸腔积液25例,经4%NaOH液化处理后用PBS洗涤并悬浮菌体,每份标本分设对照组和RPF组,按照两组各0.5 ml菌液量接种于MGIT培养管.对照组培养基采用含Middlebrook7H9的常规MGIT培养管,RPF组则在对照组基础上加入Rpf A/B/C/D/E五种蛋白混合物.接种后的所有培养管放入960全自动分枝杆菌培养系统中培养.阳性培养物行分枝杆菌菌种鉴定,记录培阳标本的报阳时间,统计培阳率.结果痰液的RPF组和对照组培阳率分别为42.86%(18/42)和45.24%(19/42);支冲液的RPF组和对照组的培阳率分别为37.5%(3/8)和62.5%(5/8);胸腔积液的RPF组和对照组的培阳率均为20%(5/25),各类标本两组间培阳率差异无统计学意义(P＞0.05).在结核培阳的标本中,9例为两组报阳天数相等;6例(胸水3例,痰2例,支冲液1例)RPF组报阳天数少于对照组,缩短天数为1～11天,平均缩短4.33天;9例RPF组报阳天数长于对照组,延长时间为1～2天,平均延长1.11天.另有3例对照组结核培阳,而RPF组培阴.结论结核杆菌复苏因子对痰液、支冲液和胸水的结核培阳率无明显提高,对胸水结核培阳时间的缩短有较明显的促进作用.复苏因子在不同标本中的复苏作用有待进一步摸索.
목적 탐토결핵간균복소인자(TB-RPF)재결핵환자표본적분지간균배양중적작용.방법 수집의사결핵환자적담액42례,지충액8례,흉강적액25례,경4%NaOH액화처리후용PBS세조병현부균체,매빈표본분설대조조화RPF조,안조량조각0.5 ml균액량접충우MGIT배양관.대조조배양기채용함Middlebrook7H9적상규MGIT배양관,RPF조칙재대조조기출상가입Rpf A/B/C/D/E오충단백혼합물.접충후적소유배양관방입960전자동분지간균배양계통중배양.양성배양물행분지간균균충감정,기록배양표본적보양시간,통계배양솔.결과 담액적RPF조화대조조배양솔분별위42.86%(18/42)화45.24%(19/42);지충액적RPF조화대조조적배양솔분별위37.5%(3/8)화62.5%(5/8);흉강적액적RPF조화대조조적배양솔균위20%(5/25),각류표본량조간배양솔차이무통계학의의(P＞0.05).재결핵배양적표본중,9례위량조보양천수상등;6례(흉수3례,담2례,지충액1례)RPF조보양천수소우대조조,축단천수위1～11천,평균축단4.33천;9례RPF조보양천수장우대조조,연장시간위1～2천,평균연장1.11천.령유3례대조조결핵배양,이RPF조배음.결론 결핵간균복소인자대담액、지충액화흉수적결핵배양솔무명현제고,대흉수결핵배양시간적축단유교명현적촉진작용.복소인자재불동표본중적복소작용유대진일보모색.
Objective To explore the role of TB-resuscitation promoting factor proteins ( TB-RPFs )in culture of mycobacterium tuberculosis. Methods 42 sputum samples, 8 samples of bronchoalveolar lavage fluid ( BALF ) and 25 samples of pleural fluid for tuberculosis patients were collected .All samples were treated with 4%NaOH, washed with PBS and then suspended. Each sample was divided into control group and RPF group. Control group was cultured in MGIT, RPF group was cultured with five types of TB-RPFs in MGIT. All samples were cultured in the automated mycobacteria testing M960 system.The cultivation time for positive cultures was recorded and the positive cultures were used to identify mycobacterium strains. Results The positive rate of sputum culture was 42.86% in the RPF group and 45.24% in the control group; that of BALF was 37.5% and 62.5%, respectively; and the positive rate of pleural fluid was 20% in both groups. There were no significant differences between the two groups. Of all the positive samples, 9 had the same days for positive results.6 samples ( 3 pleural fluid, 2 sputum, and 1 BALF ) were reported positive earlier in the RPF group than the control group, with an average length of 4.33 days but,9 were reported positive later in the RPF group group with an average length of 1.11 days. 3 samples were cultured positive in the control group but negative in the RPF group.Conclusions There is no enough evidence to prove that TB-RPFs can improve the positive rate for mycobacterium culture in sputum, BALF and pleural fluid samples. But TB-RPFs significantly shorten the positive culture time in pleural fluid. The role of TB-RPFs for culturing mycobacterium in different samples needs to be further studied.