CAJ | 학술논문

目的建立高效毛细管电泳分离检测饮料中抗坏血酸(Vc)的方法.方法实验条件;柱温20℃,电压20 kV,pH 9.2的硼酸缓冲液,进样时间10s,检测波长264 nm.同时研究缓冲液pH、电压、检测波长等对测定结果的影响.结果上述条件可实现Vc的有效分离,回收率为84.9～98.7.结论该方法简单、可重复性强,适合测定饮料中Vc.
목적 건립고효모세관전영분리검측음료중항배혈산(Vc)적방법.방법 실험조건;주온20℃,전압20 kV,pH 9.2적붕산완충액,진양시간10s,검측파장264 nm.동시연구완충액pH、전압、검측파장등대측정결과적영향.결과 상술조건가실현Vc적유효분리,회수솔위84.9～98.7.결론 해방법간단、가중복성강,괄합측정음료중Vc.
Objective To establish a procedure to quantify vitamin C in beverage samples by capillary electrophoresis.Methods The experiment was performed under the conditions:column temperature:20℃,electric voltage:20 kV,sodium borate buffer (pH 9.2),sampling time:10 s,the wave length of detection:264 nm.The buffer pH,electrophoresis voltage,the detection wavelength,et al were studied to find the influence on the detection results.Results Vc in beverage could be separated effectively under the conditions above.The recovery rate was 84.9 ～ 98.7.Conclusions The methods is simple and repeatable and is suitable to test the vitamin C in beverage.