国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2013年
3期
300-304
,共5页
黄伟青%武钊%文锐玲%刘佩仪%欧阳斌%郭伟%梁庆
黃偉青%武釗%文銳玲%劉珮儀%歐暘斌%郭偉%樑慶
황위청%무쇠%문예령%류패의%구양빈%곽위%량경
DADLE%全脑缺血再灌注%Bax%Bcl-2
DADLE%全腦缺血再灌註%Bax%Bcl-2
DADLE%전뇌결혈재관주%Bax%Bcl-2
DADLE%Whole brain ischemia reperfusion%Bax%Bcl-2
目的 观察急性全脑缺血再灌注大鼠脑组织Bax、Bcl-2蛋白表达情况,研究δ阿片受体激动剂DADLE对急性全脑缺血再灌注脑组织凋亡途径的影响以及其与脑保护作用的关系.方法 将健康雌性SD大鼠50只(180 ~ 220 g)随机分为5组:假手术组(Sham组,n=10):只暴露血管而不夹闭;缺血再灌注组(I/R组,n=10):采用改良的二血管阻断加低血压法建立全脑缺血再灌注模型,缺血15rin后给予再灌注;DADLE处理组分为DADLE2 mg/kg组(A组,n=10)、DADLE 3 mg/kg组(B组,n=10)、DADLE 5 mg/kg组(C组,n=10):在I/R组的基础上于再灌注前经颈外静脉注射DADLE.手术结束后,采用免疫组化法检测脑组织Bax、Bcl-2蛋白表达情况.结果 Sham组Bax与Bcl-2阳性细胞数显著少于其他各组(P<0.05),分别为(20.38±2.84)与(9.62±2.10);I/R组Bax与Bcl-2阳性细胞数为(52.73±6.08)与(19.49±2.35);而DADLE处理组中Bax阳性细胞数分别为:A组(43.71±3.85)、B组(37.92±4.08)、C组(36.20±3.94);Bcl-2阳性细胞数分别为:A组(28.74±2.41)、B组(33.58±2.16)、C组(34.96±1.98).Bcl-2/Bax的比值,Sham组显著高于I/R组[(49.3±3.4)%vs.(35.8±3.2)%,P<0.05];DADLE处理组中,A组为(66.4±6.8)%、B组为(85.7±7.3)%、C组为(92.6±6.9)%.结论 在大鼠急性全脑缺血再灌注损伤中,Bax与Bcl-2蛋白的表达均明显上调,而DADLE可以通过影响Bax与Bcl-2的表达程度减少细胞凋亡,发挥脑保护的作用;由Bcl-2/Bax的比值可发现,该作用与使用剂量呈现“S型剂量效应曲线”.
目的 觀察急性全腦缺血再灌註大鼠腦組織Bax、Bcl-2蛋白錶達情況,研究δ阿片受體激動劑DADLE對急性全腦缺血再灌註腦組織凋亡途徑的影響以及其與腦保護作用的關繫.方法 將健康雌性SD大鼠50隻(180 ~ 220 g)隨機分為5組:假手術組(Sham組,n=10):隻暴露血管而不夾閉;缺血再灌註組(I/R組,n=10):採用改良的二血管阻斷加低血壓法建立全腦缺血再灌註模型,缺血15rin後給予再灌註;DADLE處理組分為DADLE2 mg/kg組(A組,n=10)、DADLE 3 mg/kg組(B組,n=10)、DADLE 5 mg/kg組(C組,n=10):在I/R組的基礎上于再灌註前經頸外靜脈註射DADLE.手術結束後,採用免疫組化法檢測腦組織Bax、Bcl-2蛋白錶達情況.結果 Sham組Bax與Bcl-2暘性細胞數顯著少于其他各組(P<0.05),分彆為(20.38±2.84)與(9.62±2.10);I/R組Bax與Bcl-2暘性細胞數為(52.73±6.08)與(19.49±2.35);而DADLE處理組中Bax暘性細胞數分彆為:A組(43.71±3.85)、B組(37.92±4.08)、C組(36.20±3.94);Bcl-2暘性細胞數分彆為:A組(28.74±2.41)、B組(33.58±2.16)、C組(34.96±1.98).Bcl-2/Bax的比值,Sham組顯著高于I/R組[(49.3±3.4)%vs.(35.8±3.2)%,P<0.05];DADLE處理組中,A組為(66.4±6.8)%、B組為(85.7±7.3)%、C組為(92.6±6.9)%.結論 在大鼠急性全腦缺血再灌註損傷中,Bax與Bcl-2蛋白的錶達均明顯上調,而DADLE可以通過影響Bax與Bcl-2的錶達程度減少細胞凋亡,髮揮腦保護的作用;由Bcl-2/Bax的比值可髮現,該作用與使用劑量呈現“S型劑量效應麯線”.
목적 관찰급성전뇌결혈재관주대서뇌조직Bax、Bcl-2단백표체정황,연구δ아편수체격동제DADLE대급성전뇌결혈재관주뇌조직조망도경적영향이급기여뇌보호작용적관계.방법 장건강자성SD대서50지(180 ~ 220 g)수궤분위5조:가수술조(Sham조,n=10):지폭로혈관이불협폐;결혈재관주조(I/R조,n=10):채용개량적이혈관조단가저혈압법건립전뇌결혈재관주모형,결혈15rin후급여재관주;DADLE처리조분위DADLE2 mg/kg조(A조,n=10)、DADLE 3 mg/kg조(B조,n=10)、DADLE 5 mg/kg조(C조,n=10):재I/R조적기출상우재관주전경경외정맥주사DADLE.수술결속후,채용면역조화법검측뇌조직Bax、Bcl-2단백표체정황.결과 Sham조Bax여Bcl-2양성세포수현저소우기타각조(P<0.05),분별위(20.38±2.84)여(9.62±2.10);I/R조Bax여Bcl-2양성세포수위(52.73±6.08)여(19.49±2.35);이DADLE처리조중Bax양성세포수분별위:A조(43.71±3.85)、B조(37.92±4.08)、C조(36.20±3.94);Bcl-2양성세포수분별위:A조(28.74±2.41)、B조(33.58±2.16)、C조(34.96±1.98).Bcl-2/Bax적비치,Sham조현저고우I/R조[(49.3±3.4)%vs.(35.8±3.2)%,P<0.05];DADLE처리조중,A조위(66.4±6.8)%、B조위(85.7±7.3)%、C조위(92.6±6.9)%.결론 재대서급성전뇌결혈재관주손상중,Bax여Bcl-2단백적표체균명현상조,이DADLE가이통과영향Bax여Bcl-2적표체정도감소세포조망,발휘뇌보호적작용;유Bcl-2/Bax적비치가발현,해작용여사용제량정현“S형제량효응곡선”.
Objective To observe the expressions of Bax and Bcl-2 proteins in the brain tissue of the rats with acute cerebral ischemia reperfusion,and to explore the effect of DADLE,a delta opioid receptor agonist,on brain apoptotic pathways of acute cerebral ischemia reperfusion and the role of DADLE in the protection of brain.Methods 50 healthy female SD rats(180-220 g) were randomly divided into 5 groups:sham operation group (Sham,n =10):only exposed blood vessels without dipping; ischemia reperfusion group (I/R,n =10):modified two-vessel occlusion with hypotension to establish a model of cerebral ischemia,and reperfused 15min after ischemia; DADLE treatment group was subdivided into group of 2 mg/kg(A,n =10),group of 3mg/kg (B,n=10),and group of 5 mg/kg (C,n=10):on the basis of I/R group with reperfusion via external jugular vein injection of DADLE.Expressions of Bax and Bcl-2 proteins were detected by immnnohistochemistry assay after the operation.Results The number of cells with Bax or Bcl-2 expression (20.38 ± 2.84 and 9.62 ±-2.10,P < 0.05)was significantly smaller in Sham group than in the other groups ; In I/R group,the number of cells with Bax or Bcl-2 expression was 52.73 ± 6.08 and 19.49 ± 2.35; In DADLE treatment groups,the number of cells with Bax experssion was 43.71 ± 3.85 for group A,37.92 ± 4.08 for group B,and 36.20 ± 3.94 for group C; the number of cells with Bcl-2 expression was 28.74 ± 2.41,33.58 ± 2.16,and 34.96 ± 1.98,respectively.The ratio of Bcl-2/Bax was significantly higher in Sham group than in I/R group (49.3%-± 3.4% vs.35.8% ±3.2%,P< 0.05) ; and it was 66.4% ± 6.8% in group A,85.7% ± 7.3% in group B,and 92.6% ± 6.9% in group C.Conelusions In rats with acute cerebral ischemia reperfusion injury,expressions of Bax and Bcl-2 proteins are obviously upregulated.DADLE can play a role in protection of brain by affecting the expression degree of Bax and Bcl-2 and thus lowering apoptosis.Bcl-2\Bax ratio shows that the role and the dose is in a S-shape curve of dose-effect.