CAJ | 학술논문

目的探讨骨髓基质细胞、抗晚期抗原4抗体(aVLA-4)、阿糖胞苷(Ara-C)对白血病HL-60细胞增殖和凋亡的影响.方法实验共分为5组:HL-60细胞单独培养组(对照组),HL-60细胞+基质细胞组(基质细胞组),HL-60细胞+基质细胞+aVLA-4组(抗体组),HL-60细胞+基质细胞+Ara-C组(药物组),HL-60细胞+基质细胞+aVLA-4+Ara-C组(抗体+药物组).采用CCK-8法检测各组细胞增殖或抑制率,流式细胞术检测各组HL-60细胞凋亡情况,Western blot法检测各组HL-60细胞中抗凋亡基因bc1-2表达情况.结果基质细胞组培养24 h及48 h后HL-60细胞数量均高于对照组,差异有统计学意义[(7.2±0.3) ×105/ml比(5.3±0.4)×105/ml,(8.4±0.2)×105/ml比(6.8±0.3)×105/ml(P＜0.001)]；与基质细胞组比较,抗体组、药物组、抗体+药物组HL-60白血病细胞的增殖抑制率[(24.3±2.1)％、(37.0±2.6)％、(65.6±3.8)％]及凋亡率[(5.7±0.6)％、(8.0±0.5)％、(10.4±0.9)％、(16.5±0.7)％]均增高,差异有统计学意义(P＜0.05),并以抗体+药物组的增高最为明显；各组的bcl-2蛋白表达均有下降,以抗体+药物组的下降最明显.结论骨髓基质细胞对白血病细胞的增殖起促进作用,aVLA-4干扰基质细胞与白血病细胞间的相互作用可增强白血病细胞对Ara-C的化疗敏感性.
목적 탐토골수기질세포、항만기항원4항체(aVLA-4)、아당포감(Ara-C)대백혈병HL-60세포증식화조망적영향.방법 실험공분위5조:HL-60세포단독배양조(대조조),HL-60세포+기질세포조(기질세포조),HL-60세포+기질세포+aVLA-4조(항체조),HL-60세포+기질세포+Ara-C조(약물조),HL-60세포+기질세포+aVLA-4+Ara-C조(항체+약물조).채용CCK-8법검측각조세포증식혹억제솔,류식세포술검측각조HL-60세포조망정황,Western blot법검측각조HL-60세포중항조망기인bc1-2표체정황.결과 기질세포조배양24 h급48 h후HL-60세포수량균고우대조조,차이유통계학의의[(7.2±0.3) ×105/ml비(5.3±0.4)×105/ml,(8.4±0.2)×105/ml비(6.8±0.3)×105/ml(P＜0.001)]；여기질세포조비교,항체조、약물조、항체+약물조HL-60백혈병세포적증식억제솔[(24.3±2.1)％、(37.0±2.6)％、(65.6±3.8)％]급조망솔[(5.7±0.6)％、(8.0±0.5)％、(10.4±0.9)％、(16.5±0.7)％]균증고,차이유통계학의의(P＜0.05),병이항체+약물조적증고최위명현；각조적bcl-2단백표체균유하강,이항체+약물조적하강최명현.결론 골수기질세포대백혈병세포적증식기촉진작용,aVLA-4간우기질세포여백혈병세포간적상호작용가증강백혈병세포대Ara-C적화료민감성.
Objective To explore the bone marrow stromal cells,anti-late antigen-4 (VLA-4) antibody (aVLA-4),cytarabine (Ara-C) on the proliferation and apoptosis of leukemia HL-60 cells.Methods The experiment was divided into five groups:HL-60 cells were cultured alone (control group),HL-60 cells and stromal cells group (stromal cells group),HL-60 cells + stromal cells + aVLA-4 (antibody group),HL-60 cells + stromal cells + Ara-C group (drug group),HL-60 cells + stromal cells + aVLA-4 + Ara-C group (antibody +drug group).Cell proliferation or inhibition rate was detected by CCK-8 method,the HL-60 cells apoptosis was detected by flow cytometry.The expression of anti-apoptotic gene bcl-2 in HL-60 cells was determined by Western blot.Results After 24 h and 48 h,treatment,the number of the stromal cells group HL-60 cells were higher than that of the control group with significant difference cultured [(7.2±0.3)×1O5/ml vs (5.3±0.4)×105/ml,(8.4±0.2)×105/ml vs (6.8±0.3)×105/m1,P ＜ 0.001],while the HL-60 cell proliferation inhibition rate [(24.3±2.1) ％,(37.0±2.6) ％,(65.6±3.8) ％] and apoptosis rate [(5.7±0.6) ％,(8.0±0.5) ％,(10.4±0.9) ％,(16.5±0.7) ％] of antibody group,drug group,antibody + drug group were higher than the control group with a difference of statistically significant (P ＜ 0.05),and the increase of antibody + drug group was most obvious.With the decreasing of the bcl-2 protein expression,which was most the decrease of antibody + drug group was most obvious.Conclusion Bone marrow stromal cells can stimulate the proliferation of leukemia cells,aVLA-4 interference the interaction between stromal cells and leukemia cells can enhance the chemosensitivity of leukemia cells to Ara-C.