国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2013年
5期
616-619
,共4页
唐菊英%黎锋%戚以琴%李娜%张少玲
唐菊英%黎鋒%慼以琴%李娜%張少玲
당국영%려봉%척이금%리나%장소령
唾液皮质醇%库欣综合征%酶联免疫吸附分析
唾液皮質醇%庫訢綜閤徵%酶聯免疫吸附分析
타액피질순%고흔종합정%매련면역흡부분석
Salivary cortisol(SC)%Cushing's Syndrome (CS)%ELISA
目的 比较分析夜间23:00与24:00唾液皮质醇检测结果,以判断能否将午夜唾液皮质醇提前至23:00采集,用于临床库欣综合征的筛查诊断.方法 对健康人群组(NS组)108例,库欣综合征(CS)高危人群组(NCS组)50例,库欣综合征患者(CS组)7例,分别于夜间23:00与24:00采集唾液,用酶联免疫吸附分析法(ELISA)检测唾液皮质醇.结果 三组受试者夜间23:00与24:00唾液皮质醇检测结果分别为:NS组:(2.01±0.91)ng/ml,(1.96±0.85)ng/ml; NCS组:(3.55±1.73)ng/ml,(3.81±1.49) ng/ml; CS组:(12.63±6.14)ng/ml,(10.98±3.98)ng/ml；三组组内两时间唾液皮质醇检测结果差异均无统计学意义(P均＞0.05),呈良好的直线相关(r=0.924,P＜ 0.001)；三组组间两时间唾液皮质醇检测结果差异均有统计学意义(P均＜0.001),CS组明显高于NS组和NCS组,NCS组高于NS组；23:00和24:00唾液皮质醇ROC曲线下面积分别为:0.995、0.996,对CS的诊断准确性较高.结论 夜间唾液皮质醇浓度是筛查、诊断CS的一个方便、敏感指标；23:00唾液皮质醇浓度与24:00的接近,在23:00采集唾液可以满足临床库欣综合征的筛查诊断要求.
目的 比較分析夜間23:00與24:00唾液皮質醇檢測結果,以判斷能否將午夜唾液皮質醇提前至23:00採集,用于臨床庫訢綜閤徵的篩查診斷.方法 對健康人群組(NS組)108例,庫訢綜閤徵(CS)高危人群組(NCS組)50例,庫訢綜閤徵患者(CS組)7例,分彆于夜間23:00與24:00採集唾液,用酶聯免疫吸附分析法(ELISA)檢測唾液皮質醇.結果 三組受試者夜間23:00與24:00唾液皮質醇檢測結果分彆為:NS組:(2.01±0.91)ng/ml,(1.96±0.85)ng/ml; NCS組:(3.55±1.73)ng/ml,(3.81±1.49) ng/ml; CS組:(12.63±6.14)ng/ml,(10.98±3.98)ng/ml；三組組內兩時間唾液皮質醇檢測結果差異均無統計學意義(P均＞0.05),呈良好的直線相關(r=0.924,P＜ 0.001)；三組組間兩時間唾液皮質醇檢測結果差異均有統計學意義(P均＜0.001),CS組明顯高于NS組和NCS組,NCS組高于NS組；23:00和24:00唾液皮質醇ROC麯線下麵積分彆為:0.995、0.996,對CS的診斷準確性較高.結論 夜間唾液皮質醇濃度是篩查、診斷CS的一箇方便、敏感指標；23:00唾液皮質醇濃度與24:00的接近,在23:00採集唾液可以滿足臨床庫訢綜閤徵的篩查診斷要求.
목적 비교분석야간23:00여24:00타액피질순검측결과,이판단능부장오야타액피질순제전지23:00채집,용우림상고흔종합정적사사진단.방법 대건강인군조(NS조)108례,고흔종합정(CS)고위인군조(NCS조)50례,고흔종합정환자(CS조)7례,분별우야간23:00여24:00채집타액,용매련면역흡부분석법(ELISA)검측타액피질순.결과 삼조수시자야간23:00여24:00타액피질순검측결과분별위:NS조:(2.01±0.91)ng/ml,(1.96±0.85)ng/ml; NCS조:(3.55±1.73)ng/ml,(3.81±1.49) ng/ml; CS조:(12.63±6.14)ng/ml,(10.98±3.98)ng/ml；삼조조내량시간타액피질순검측결과차이균무통계학의의(P균＞0.05),정량호적직선상관(r=0.924,P＜ 0.001)；삼조조간량시간타액피질순검측결과차이균유통계학의의(P균＜0.001),CS조명현고우NS조화NCS조,NCS조고우NS조；23:00화24:00타액피질순ROC곡선하면적분별위:0.995、0.996,대CS적진단준학성교고.결론 야간타액피질순농도시사사、진단CS적일개방편、민감지표；23:00타액피질순농도여24:00적접근,재23:00채집타액가이만족림상고흔종합정적사사진단요구.
Objective To compare the detection results of salivary cortisol(SL) at 23:00 and 24:00 to estimate whether the collection of saliva can be taken in advance at 23:00 for the screening and diagnosis of Cushing's syndrome(CS).Methods Saliva samples were collected by a cotton-based sampling approaches Salivettes at 23:00 and 24:00 from 108 normal subjects (NS group),50 patients (NCS group) with type 2 diabetes,primary hypertension or non-functional adrenal adenoma and 7 patients with Cushing' s syndrome(CS group).Then the samples were assayed by ELISA.Results The levels of SC at 23:00 (SC23) and 24:00 (SC24) in different groups were:2.01 ± 0.91 ng/ml,1.96 ± 0.85 ng/ml in NS group,3.55 ± 1.17 ng/ml,3.81 ± 1.49 ng/ml in NCS group,and 12.63 ± 6.14 ng/ml,10.98 ± 3.98 ng/ml in CS group (all P＞0.05).Using Pearson correlation analysis,SC23 and SC24 showed a very good linear correlation (r =0.924,P＜ 0.001).SC23 or SC24 in CS group were obviously higher than NS and NCS group.The areas under the ROC curves of SC23 and SC24 were 0.995 and 0.996 respectively,which displayed good accuracy to diagnosis of CS.Conclusions Midnight salivary cortisol is a convenient and sensitive indicator for the screening and diagnosis of CS.The concentrations of salivary cortisol at 23:00 and 24:00 are close,so collecting saliva at 23:00 is feasible for the clinical screening and diagnosis of CS.
