国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2013年
18期
2827-2833
,共7页
史贵秀%孙丽华%张跃新%杨艳%孟令梅
史貴秀%孫麗華%張躍新%楊豔%孟令梅
사귀수%손려화%장약신%양염%맹령매
骨髓间充质干细胞%5-乙炔基-2’脱氧尿嘧啶核苷%急性肝衰竭%移植%增殖
骨髓間充質榦細胞%5-乙炔基-2’脫氧尿嘧啶覈苷%急性肝衰竭%移植%增殖
골수간충질간세포%5-을결기-2’탈양뇨밀정핵감%급성간쇠갈%이식%증식
Bone marrow mesenchymal stem cell%5 ethynyl-2 deoxyuridine%Acute hepatic failure%Transplantation%Proliferation
目的 探讨骨髓间充质干细胞治疗急性肝衰竭的机制.方法 全骨髓贴壁法培养扩增原代SD大鼠骨髓干细胞,传代培养,形态学、生长曲线及免疫表型鉴定骨髓间充质干细胞,移植治疗D-氨基半乳糖联合脂多糖致肝衰竭大鼠,观察干预治疗后1d、3d、5d、7d肝组织病理学改变、肝组织PCNA蛋白、CyclinD1mRNA表达量.结果 原代培养大鼠骨髓间充质干细胞传代后,形态较均一,为梭形或纺锤形,高表达CD90(94.3％),CD29(99.4％),不表达CD11b/c(5.7％),CD45(3.2％).骨髓间充质干细胞移植减轻肝脏组织病理损伤(P＜0.05).BMSCs治疗组PCNA的蛋白表达高于对照组(P＜0.01).干预后不同时间点治疗组细胞周期蛋白D1的mRNA表达高于对照组(1.182±0.248 vs 0.468±0.053、0.858±0.114 vs 0.218±0.178),差异有统计学意义(P＜0.01).结论 骨髓间充质干细胞移植减轻肝脏病理损伤,上调细胞周期蛋白D1mRNA的表达,促进肝再生,对急性肝衰竭有治疗作用.
目的 探討骨髓間充質榦細胞治療急性肝衰竭的機製.方法 全骨髓貼壁法培養擴增原代SD大鼠骨髓榦細胞,傳代培養,形態學、生長麯線及免疫錶型鑒定骨髓間充質榦細胞,移植治療D-氨基半乳糖聯閤脂多糖緻肝衰竭大鼠,觀察榦預治療後1d、3d、5d、7d肝組織病理學改變、肝組織PCNA蛋白、CyclinD1mRNA錶達量.結果 原代培養大鼠骨髓間充質榦細胞傳代後,形態較均一,為梭形或紡錘形,高錶達CD90(94.3％),CD29(99.4％),不錶達CD11b/c(5.7％),CD45(3.2％).骨髓間充質榦細胞移植減輕肝髒組織病理損傷(P＜0.05).BMSCs治療組PCNA的蛋白錶達高于對照組(P＜0.01).榦預後不同時間點治療組細胞週期蛋白D1的mRNA錶達高于對照組(1.182±0.248 vs 0.468±0.053、0.858±0.114 vs 0.218±0.178),差異有統計學意義(P＜0.01).結論 骨髓間充質榦細胞移植減輕肝髒病理損傷,上調細胞週期蛋白D1mRNA的錶達,促進肝再生,對急性肝衰竭有治療作用.
목적 탐토골수간충질간세포치료급성간쇠갈적궤제.방법 전골수첩벽법배양확증원대SD대서골수간세포,전대배양,형태학、생장곡선급면역표형감정골수간충질간세포,이식치료D-안기반유당연합지다당치간쇠갈대서,관찰간예치료후1d、3d、5d、7d간조직병이학개변、간조직PCNA단백、CyclinD1mRNA표체량.결과 원대배양대서골수간충질간세포전대후,형태교균일,위사형혹방추형,고표체CD90(94.3％),CD29(99.4％),불표체CD11b/c(5.7％),CD45(3.2％).골수간충질간세포이식감경간장조직병리손상(P＜0.05).BMSCs치료조PCNA적단백표체고우대조조(P＜0.01).간예후불동시간점치료조세포주기단백D1적mRNA표체고우대조조(1.182±0.248 vs 0.468±0.053、0.858±0.114 vs 0.218±0.178),차이유통계학의의(P＜0.01).결론 골수간충질간세포이식감경간장병리손상,상조세포주기단백D1mRNA적표체,촉진간재생,대급성간쇠갈유치료작용.
Objective To explore the mechanism of transplantation of bone marrow mesenchymal stem cells in the treatment of acute liver failure.Methods Cultured and amplified the rat bone marrow mesenchymal stem cells by adherent culture method from primary generation to fifth generation.Acute hepatic failure was induced by two injections of D-galactosamine and one injection of Lipopolysaccharide,delivered intraperitoneally with a 12-hour interval between injections.1 ml BMSCs 1.4 × 107/kg or 0.9％ NaCl solution (control) was infused into caudal vein.PCNA in liver tissue was detected by immunohistochemistry.The level of CyclinD1 mRNA in liver tissue was observed.Results BMSCs in vitro culture were homogenous population and exhibited a spindle-shaped morphology.BMSCs expressed CD29 and CD90,didn' t express CD1 lb or CD45.BMSCs therapy inhibited liver enzyme release (P ＜ 0.05).BMSCs therapy improved gross and microscopic liver histopathology (P ＜ 0.05).BMSCs therapy enhanced liver regeneration (P ＜ 0.05).BMSCs therapy up-regulated the expression of CyclinD1 mRNA (P ＜ 0.05).Conclusion BMSCs promote the regeneration of liver tissues.BMSCs transplantation has a therapy effect on acute liver failure.
