国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
13期
1866-1869
,共4页
许衍硕%陈志斌%李凡%谢亦欢%叶国辉%唐皓
許衍碩%陳誌斌%李凡%謝亦歡%葉國輝%唐皓
허연석%진지빈%리범%사역환%협국휘%당호
丙氨酰-谷氨酰胺%慢性阻塞性肺病急性加重期%CD4+、CD8+淋巴细胞%炎症介质
丙氨酰-穀氨酰胺%慢性阻塞性肺病急性加重期%CD4+、CD8+淋巴細胞%炎癥介質
병안선-곡안선알%만성조새성폐병급성가중기%CD4+、CD8+림파세포%염증개질
Alanyl-glutamine%Acute exacerbation of chronic obstructive pulmonary disease%CD4+,CD8+ lymphocyte%Mediators of inflammation
目的 观察静脉补充丙氨酰-谷氨酰胺(AG)对慢性阻塞性肺疾病急性加重(acuteexacerbation of chronic obstructive pulmonary disease,AECOPD)患者CD4+/CD8+T细胞及IL-8、TNF-α的影响.方法 通过静脉对AECOPD患者补充丙氨酰-谷氨酰胺,ELISA法检测IL-8、TNF-α表达水平,流式细胞仪检测CD4+、CD8+、CD3+淋巴细胞变化.结果 与对照组比较,丙氨酰-谷氨酰胺治疗组(AG组)临床缓解率高于对照组(93.33％vs 73.33％);治疗后AG组IL-8[(102.11±13.57) ng/ml]、TNF-α[(0.83±0.26)μg/L]水平低于对照组[(137.46±12.9) ng/ml、(1.34±0.42)μg/L],两组比较差异有统计学意义(P＜0.05);治疗后,两组CD4+/CD8+比值比较差异无统计学意义,但AG组CD3+淋巴细胞绝对值(0.69×109/L)高于对照组(0.50×109/L) (P＜0.05).结论 谷氨酰胺可促进AECOPD患者T淋巴细胞的增殖,并同时减少IL-8、TNF-α的表达,从而可能对AECOPD起到治疗作用.
目的 觀察靜脈補充丙氨酰-穀氨酰胺(AG)對慢性阻塞性肺疾病急性加重(acuteexacerbation of chronic obstructive pulmonary disease,AECOPD)患者CD4+/CD8+T細胞及IL-8、TNF-α的影響.方法 通過靜脈對AECOPD患者補充丙氨酰-穀氨酰胺,ELISA法檢測IL-8、TNF-α錶達水平,流式細胞儀檢測CD4+、CD8+、CD3+淋巴細胞變化.結果 與對照組比較,丙氨酰-穀氨酰胺治療組(AG組)臨床緩解率高于對照組(93.33％vs 73.33％);治療後AG組IL-8[(102.11±13.57) ng/ml]、TNF-α[(0.83±0.26)μg/L]水平低于對照組[(137.46±12.9) ng/ml、(1.34±0.42)μg/L],兩組比較差異有統計學意義(P＜0.05);治療後,兩組CD4+/CD8+比值比較差異無統計學意義,但AG組CD3+淋巴細胞絕對值(0.69×109/L)高于對照組(0.50×109/L) (P＜0.05).結論 穀氨酰胺可促進AECOPD患者T淋巴細胞的增殖,併同時減少IL-8、TNF-α的錶達,從而可能對AECOPD起到治療作用.
목적 관찰정맥보충병안선-곡안선알(AG)대만성조새성폐질병급성가중(acuteexacerbation of chronic obstructive pulmonary disease,AECOPD)환자CD4+/CD8+T세포급IL-8、TNF-α적영향.방법 통과정맥대AECOPD환자보충병안선-곡안선알,ELISA법검측IL-8、TNF-α표체수평,류식세포의검측CD4+、CD8+、CD3+림파세포변화.결과 여대조조비교,병안선-곡안선알치료조(AG조)림상완해솔고우대조조(93.33％vs 73.33％);치료후AG조IL-8[(102.11±13.57) ng/ml]、TNF-α[(0.83±0.26)μg/L]수평저우대조조[(137.46±12.9) ng/ml、(1.34±0.42)μg/L],량조비교차이유통계학의의(P＜0.05);치료후,량조CD4+/CD8+비치비교차이무통계학의의,단AG조CD3+림파세포절대치(0.69×109/L)고우대조조(0.50×109/L) (P＜0.05).결론 곡안선알가촉진AECOPD환자T림파세포적증식,병동시감소IL-8、TNF-α적표체,종이가능대AECOPD기도치료작용.
Objective To observe the changes of CD4+,CD8+ lymphocyte,IL-8 and TNF-o of patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) after intravenous feeding of alanylglutamine.Methods We supplemented alanyl-glutamine by phleboclysis for AECOPD patients.The level of IL8,TNF-α were tested by enzyme-linked immunosorbent assay (ELISA),and CD4+ and CD8+ lymphocyte in peripheral blood were detected by flowcytometry in AECOPD patients.SPSS 13.0 software was used to analyze the data.Results Compared to control group,the rate of clinical remission in alanyl-glutamine therapy group was higher (93.33％ vs 73.33％).On the 7th day,the levels of IL-8 (102.11 ± 13.57) ng/ml TNF-α (0.83 ± 0.26) μ g/ L in AG therapy group were lower than those in control group (137.46 ± 12.9) ng/ml and (1.34 ± 0.42) μ g/L,all P ＜ 0.05; but the number of CD3+ lymphocyte in AG therapy group (0.69 × 109)/L was more than that of control group (0.50 × 109)/L,P ＜ 0.05.There was no statistical significant difference in the ratio of CD4+/CD8+ lymphocyte between AG therapy group and control group.Conclusion Glutamine can boost the proliferation of T lymphocyte,at the same time,reduce the expression of IL-8 and TNF-o,thus making it have treatment effect on AECOPD.
